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Cell No. : Cell Name
RCB1897 : K562  update : 2024/01/17
CommentHuman cell line derived from chronic myelogenous leukemia. IMPORTANT NOTICE: Can NOT produce hemoglobin in the presence of NaB. Of note, K562 (RCB0027) and K-562 (RCB1635) can produce hemoglobin in the presence of NaB. TKG0210 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Lozzio, C.B. & Lozzio, B.B.
Year of deposit 2004
Original cell TKG0210
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Disease name chronic myelogenous leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0210)
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_0004
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Isozyme LD, NP
Reference information Reference 32
User's Publication 16

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117  Baker EJ, Ichiki AT, Day NE, Andrews RB, Bamberger EG, Lozzio CB.  Simultaneous flow cytometric measurement of K-562 megakaryocytic differentiation and CD56+ large granular lymphocyte cytotoxicity  J Immunol Methods  2001  253(1-2):37-44  PubMed ID: 11384667  
98  Baker EJ, Ichiki AT, Hodge JW, Sugantharaj D, Bamberger EG, Lozzio CB.  PMA-treated K-562 leukemia cells mediate a TH2-specific expansion of CD4+ T cells in vitro  Leuk Res  2000  24(12):1049-57.  PubMed ID: 11077119   DOI: 10.1016/s0145-2126(00)00082-5
97  Ichiki AT, Langenberg M, Baker EJ, Hodge JW, Bamberger EG, Gerard DA, Lozzio CB.  Differential regulation of interleukin-1alpha and interleukin-1beta in K-562 cells  J Interferon Cytokine Res  1998  18(12):1045-50  PubMed ID: 9877448   DOI: 10.1089/jir.1998.18.1045
101  Wust CJ, Hodge JW, Ichiki AT, Lozzio CB.  Cell death in the human leukemia cell line, K-562, induced by antiserum and monoclonal antibodies  Leuk Res  1991  15(6):497-506  PubMed ID: 1713636   DOI: 10.1016/0145-2126(91)90061-w
103  Ichiki AT, Sugantharaj DG, Hudson CS, Gerard DA, Barnhill MA, Bamberger EG, Wust CJ, Fuhr JE, Lozzio CB.  Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis  Nat Immun Cell Growth Regul  1988  7(5-6):297-304  PubMed ID: 3146698  
106  Walter H, Krob EJ, Al-Romaihi FA, Johnson D, Lozzio CB.  Detection of surface differences between closely related cell populations by partitioning. Cultured K-562 cell sublines  Cell Biophys  1988  13(3):173-87  PubMed ID: 2465826   DOI: 10.1007/BF02918374
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
95  Ichiki AT, Bamberger EG, Wust CJ, Lozzio CB.  Diversity of cell surface hematopoietic antigens on K-562 sublines identified with monoclonal antibodies  Leuk Res  1986  10(5):565-74  PubMed ID: 2940421   DOI: 10.1016/0145-2126(86)90092-5
96  Ichiki AT, Wust CJ, Lozzio CB.  Characterization of the Fc(IgG) receptor on the pluripotential leukaemia cell K-562  Clin Exp Immunol  1985  59(1):64-8  PubMed ID: 3971599  
116  Rowley PT, Ohlsson-Wilhelm BM, Wisniewski L, Lozzio CB, Lozzio BB.  K562 human leukemia cell passages differ in embryonic globin gene expression  Leuk Res  1984  8(1):45-54  PubMed ID: 6199625  
100  Lozzio BB, Machado EA, Mitchell J, Lozzio CB, Wust CJ, Golde DW.  Proliferation of human malignant hematopoietic cells in immunodeficient mice: suppression by antibody to pluripotent K-562 leukemia cells involves direct cytolysis and effector cells  Blood  1983  61(6):1045-53  PubMed ID: 6132631  
94  Wust CJ, Green M, Lozzio CB, Lozzio BB.  Antibody-dependent cytolysis of the human leukemia cell line K-562 in the absence of effector cells or complement  Blood  1982  59(1):133-40  PubMed ID: 7053760  
102  Ichiki AT, Lozzio BB, Wust CJ, Lozzio CB.  Relationship between the Fc receptor for IgG and the specific associated antigen of the pluripotential leukaemia cell line, K-562  Clin Exp Immunol  1982  50(2):360-5  PubMed ID: 6185259  
105  Machado EA, Gerard DA, Mitchell JR, Lozzio BB, Lozzio CB.  Arrest and extravasation of neoplastic cells. An electron microscopy study of serial sections at sequential stages  Virchows Arch A Pathol Anat Histol  1982  396(1):73-89  PubMed ID: 7123846   DOI: 10.1007/BF00428501
107  Lozzio CB, Lozzio BB, Wust CJ, Bamberger EG, Golde DW, Minowada J.  Antibody lysis of human hematopoietic cells in the absence of complement and effector cells  Exp Hematol  1982  10(8):698-707  PubMed ID: 6183138  
115  Perez RL, Mitchell JR, Lozzio BB.  Relevance of lipids to heterotransplantation of human malignancies  Oncology  1982  39(3):179-84  PubMed ID: 7043353   DOI: 10.1159/000225632
93  Fuhr JE, Bamberger EG, Lozzio CB, Lozzio BB.  Induction of hemoglobin synthesis in original K 562 cell line  Blood Cells  1981  7(2):389-99  PubMed ID: 6170369  
108  Latif ZA, Lozzio BB, Wust CJ, Krauss S, Aggio MC, Lozzio CB.  Evaluation of drug-antibody conjugates in the treatment of human myelosarcomas transplanted in nude mice  Cancer  1980  45(6):1326-33  PubMed ID: 6928397   DOI: 10.1002/1097-0142(19800315)45:6<1326::aid-cncr2820450610>;2-y
113  Lair SV, Lozzio BB.  Thymocyte reconstitution of athymic and athymic-asplenic mice: graft rejection and antibody synthesis  Exp Cell Biol  1980  48(6):439-44  PubMed ID: 6967835   DOI: 10.1159/000163010
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026   DOI: 10.1016/0145-2126(79)90033-x
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354   DOI: 10.1038/280164a0
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644   DOI: 10.1038/278364a0
89  Lozzio BB, Machado EA, Lair SV, Lozzio CB.  Reproducible metastatic growth of K-562 human myelogenous leukemia cells in nude mice  J Natl Cancer Inst  1979  63(2):295-9  PubMed ID: 287825  
112  Lozzio CB, Lozzio BB, Wust CJ, Kim J.  Correlation of leukemia-associated antigens and Ph1 chromosome in fibroblastlike cells derived from bone marrow  Blood  1978  52(4):673-80  PubMed ID: 99197  
114  Lozzio BB, Lozzio CB, Bamberger EG.  Cytotoxicity of a factor from normal and abnormal human spleens for allogenic leukemia cells  Am J Hematol  1978  4(1):57-65  PubMed ID: 655158   DOI: 10.1002/ajh.2830040108
99  Collins JL, Wust CJ, Lozzio BB, Lozzio CB.  Characterization of an antigen from the myelogenous leukemia cell line K-562  J Natl Cancer Inst  1977  59(6):1667-74  PubMed ID: 72824   DOI: 10.1093/jnci/59.6.1667
109  Lozzio BB, Lozzio CB, Krauss S, Wust CJ, Girardi A.  Leukemia-associated antigens detected by a nonhuman primate antiserum to a Ph1+ myeologenous leukemia cell line  Blood  1977  50(1):115-24  PubMed ID: 68791  
111  Lozzio BB, Machado EA, Lair SA, Lozzio CB.  Suppression of human myelosarcoma growth in athymic mice by a primate antiserum  Cancer Treat Rep  1977  61(9):1679-84  PubMed ID: 271518  
88  Lozzio CB, Lozzio BB, Yang WK, Ichiki AT, Bamberger EG.  Absence of thymus-derived lymphocyte markers in myelogenous leukemia (Ph1+) cell line K-562  Cancer Res  1976  36(12):4657-62  PubMed ID: 63324  
104  Lozzio BB, Lozzi CB, Machado E.  Human myelogenous (Ph+) leukemia cell line: transplantation into athymic mice  J Natl Cancer Inst  1976  56(3):627-9  PubMed ID: 1062625   DOI: 10.1093/jnci/56.3.627
110  Lozzio BB, Machado EA, Lozzio CB, Lair S.  Hereditary asplenic-athymic mice: transplantation of human myelogenous leukemic cells  J Exp Med  1976  143(1):225-31  PubMed ID: 53268   DOI: 10.1084/jem.143.1.225
4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658  

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User's Publication
21671  Mi D, Yanatori I, Zheng H, Kong Y, Hirayama T, Toyokuni S.  Association of poly(rC)-binding protein-2 with sideroflexin-3 through TOM20 as an iron entry pathway to mitochondria.  Free Radic Res  2024  1-40  PubMed ID: 38599240   DOI: 10.1080/10715762.2024.2340711
21717  Kasai F, Mizukoshi K, Nakamura Y.  Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature  Sci Rep  2024  14(1):9619  PubMed ID: 38671192   DOI: 10.1038/s41598-024-60271-8
18139  Hoshino N, Kanadome T, Takasugi T, Itoh M, Kaneko R, Inoue YU, Inoue T, Hirabayashi T, Watanabe M, Matsuda T, Nagai T, Tarusawa E, Yagi T.  Visualization of trans homophilic interaction of clustered protocadherin in neurons  Proc Natl Acad Sci U S A  2023  120(38):e2301003120  PubMed ID: 37695902   DOI: 10.1073/pnas.2301003120
21353  Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T.  Visualization of trans-interactions of a protocadherin-α between processes originating from single neurons.  iScience  2023  26(7):107238  PubMed ID: 37534169   DOI: 10.1016/j.isci.2023.107238
20321  Izumi K, Shindo T, Ngo HT, Nakayama-Hosoya K, Akahane K, Tamai M, Nguyen TTT, Kawana-Tachikawa A, Inukai T, Takaori-Kondo A.  KIR3DL1 Allotype-Dependent Modulation of NK Cell Immunity against Chronic Myeloid Leukemia.  Immunohorizons  2021  5(8):687-702  PubMed ID: 34433624   DOI: 10.4049/immunohorizons.2100054
20440  Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T.  Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin.  Sci Rep  2021  11(1):22237  PubMed ID: 34782670   DOI: 10.1038/s41598-021-01481-2
18206  Viriyaadhammaa N, Saiai A, Neimkhum W, Nirachonkul W, Chaiyana W, Chiampanichayakul S, Tima S, Usuki T, Duangmano S, Anuchapreeda S.  Cytotoxic and Antiproliferative Effects of Diarylheptanoids Isolated from Curcuma comosa Rhizomes on Leukaemic Cells  Molecules  2020  25(22):5476  PubMed ID: 33238470   DOI: 10.3390/molecules25225476
11912  Chen Z, Nishimura N, Okamoto T, Wada K, Naora K.  Molecular Mechanism of Matrine from Sophora alopecuroides in the Reversing Effect of Multi-Anticancer Drug Resistance in K562/ADR Cells.  Biomed Res Int  2019    PubMed ID: 31871929   DOI: 10.1155/2019/1269532
12489  Anuchapreeda S, Rungrojsakul M, Tima S, Chiampanichayakul S, Krig SR.  Co-activation of WT1 and AP-1 proteins on WT1 gene promoter to induce WT1 gene expression in K562 cells  Cell Signal  2019  53:339-347  PubMed ID: 30395942   DOI: 10.1016/j.cellsig.2018.11.001
4269  Hirao T, Yamaguchi M, Kikuya M, Chibana H, Ito K, Aoki S.  Altered intracellular signaling by imatinib increases the anti-cancer effects of tyrosine kinase inhibitors in chronic myelogenous leukemia cells.  Cancer Sci.  2018    PubMed ID: 29121435   DOI: 10.1111/cas.13442
14533  Zhi Chen, Nobuhiro Nishimura, Takayuki Okamoto, Koichiro Wada, Kohji Naora  ABCB1-mediated multi-drug resistance in leukemic K562/ADR cells was reversed by Matrine, the main ingredient from Sophora alopecuroides  Proceedings for Annual Meeting of The Japanese Pharmacological Society  2018  WCP2018:PO3-7-14    DOI: 10.1254/jpssuppl.wcp2018.0_po3-7-14
13998  Nakai W, Yoshida T, Diez D, Miyatake Y, Nishibu T, Imawaka N, Naruse K, Sadamura Y, Hanayama R.  A novel affinity-based method for the isolation of highly purified extracellular vesicles  Sci Rep  2016  6:33935  PubMed ID: 27659060   DOI: 10.1038/srep33935
14001  Bologna-Molina R, Takeda Y, Kuga T, Chosa N, Kitagawa M, Takata T, Ishisaki A, Mikami T.  Expression of Wilms' tumor 1 (WT1) in ameloblastomas  J Oral Sci  2016  58(3):407-13  PubMed ID: 27665981   DOI: 10.2334/josnusd.15-0546
13885  Umsumarng S, Pitchakarn P, Sastraruji K, Yodkeeree S, Ung AT, Pyne SG, Limtrakul P.  Reversal of human multi-drug resistance leukaemic cells by stemofoline derivatives via inhibition of P-glycoprotein function  Basic Clin Pharmacol Toxicol  2015  116(5):390-7  PubMed ID: 25265513   DOI: 10.1111/bcpt.12331
18179  Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, Nishio J, Negishi H, Tamura T, Saijo S, Iwakura Y, Taniguchi T.  Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses  Elife  2014  3:e04177  PubMed ID: 25149452   DOI: 10.7554/eLife.04177
13882  Yamagishi N, Nakao R, Kondo R, Nishitsuji M, Saito Y, Kuga T, Hatayama T, Nakayama Y.  Increased expression of sorcin is associated with multidrug resistance in leukemia cells via up-regulation of MDR1 expression through cAMP response element-binding protein  Biochem Biophys Res Commun  2014  448(4):430-6  PubMed ID: 24796664   DOI: 10.1016/j.bbrc.2014.04.125

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