Cell No. : Cell Name
RCB1897 : K562 update : 2021/01/08 |
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Comment | Human cell line derived from chronic myelogenous leukemia. Differentiate into mature erythrocytes. TKG0210 (Deposited from Tohoku Univ.). | |||
Comment from the depositor | ||||
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
Remarks | ||||
Order Form | Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use. | |||
Basic information | Depositor | Obinata, Masuo | ||
Originator | Lozzio, C.B. & Lozzio, B.B. | |||
Year of deposit | 2004 | |||
Original cell | TKG0210 | |||
Animal | human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Gender | Female | |||
Age at sampling | 53 years | |||
Tissue | pleural effusion | |||
Case history | chronic myelogenous leukemia | |||
Classification | cancer | |||
History | Cell Resource Center for Biomedical Research, Tohoku University(TKG0210) | Lifespan | infinite | |
Morphology | lymphoblast-like | |||
Medium | Medium List | Culture type | Suspension cells | |
Medium and additives | RPMI1640 + 10% FBS | |||
Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Virus (HIV) | LT 400 | |||
Isozyme | LD, NP | |||
Reference information | Reference | 5 | ||
User's Publication | 3 | |||