Cell No. : Cell Name RCB1897 : K562  update : 2021/01/08
Comment			Human cell line derived from chronic myelogenous leukemia. Differentiate into mature erythrocytes. TKG0210 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
	Basic information	Depositor	Obinata, Masuo
		Originator	Lozzio, C.B. & Lozzio, B.B.
		Year of deposit	2004
		Original cell	TKG0210
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	53 years
		Tissue	pleural effusion
		Case history	chronic myelogenous leukemia
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0210）
		Lifespan	infinite
		Morphology	lymphoblast-like
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Medium and additives		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		LT 400
		Isozyme		LD, NP
Reference information		Reference	5
		User's Publication	3

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Reference

5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004

4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026

969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354

970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644

4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658

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User's Publication

12489  Anuchapreeda S, Rungrojsakul M, Tima S, Chiampanichayakul S, Krig SR.  Co-activation of WT1 and AP-1 proteins on WT1 gene promoter to induce WT1 gene expression in K562 cells  Cell Signal  2019  53:339-347  PubMed ID: 30395942

4269  Hirao T, Yamaguchi M, Kikuya M, Chibana H, Ito K, Aoki S.  Altered intracellular signaling by imatinib increases the anti-cancer effects of tyrosine kinase inhibitors in chronic myelogenous leukemia cells.  Cancer Sci.  2018    PubMed ID: 29121435

7421  Zhang B, Shimada Y, Kuroyanagi J, Ariyoshi M, Nomoto T, Shintou T, Umemoto N, Nishimura Y, Miyazaki T, Tanaka T.  In vivo selective imaging and inhibition of leukemia stem-like cells using the fluorescent carbocyanine derivative, DiOC5(3)  Biomaterials  2015  52:14-25  PubMed ID: 25818410