Back Back
Cell No. : Cell Name
RCB0027 : K562  update : 2024/06/11
CommentHuman cell line derived from chronic myelogenous leukemia. Can be a target of NK cells (reference). Can produce hemoglobin in the presence of NaB.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kanazawa, Satoshi
Originator Lozzio, C.B. & Lozzio, B.B.
Year of deposit 1986
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Disease name chronic myelogenous leukemia
Classification cancer
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_0004
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium HamF12 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 59-68(50) : /63(5),64(7),65(6),66(8),67(16),68(5)
STR(human) OK
deposit info
lot info
Reference information Reference 33
User's Publication 101

To topTop
117  Baker EJ, Ichiki AT, Day NE, Andrews RB, Bamberger EG, Lozzio CB.  Simultaneous flow cytometric measurement of K-562 megakaryocytic differentiation and CD56+ large granular lymphocyte cytotoxicity  J Immunol Methods  2001  253(1-2):37-44  PubMed ID: 11384667  
98  Baker EJ, Ichiki AT, Hodge JW, Sugantharaj D, Bamberger EG, Lozzio CB.  PMA-treated K-562 leukemia cells mediate a TH2-specific expansion of CD4+ T cells in vitro  Leuk Res  2000  24(12):1049-57.  PubMed ID: 11077119   DOI: 10.1016/s0145-2126(00)00082-5
97  Ichiki AT, Langenberg M, Baker EJ, Hodge JW, Bamberger EG, Gerard DA, Lozzio CB.  Differential regulation of interleukin-1alpha and interleukin-1beta in K-562 cells  J Interferon Cytokine Res  1998  18(12):1045-50  PubMed ID: 9877448   DOI: 10.1089/jir.1998.18.1045
101  Wust CJ, Hodge JW, Ichiki AT, Lozzio CB.  Cell death in the human leukemia cell line, K-562, induced by antiserum and monoclonal antibodies  Leuk Res  1991  15(6):497-506  PubMed ID: 1713636   DOI: 10.1016/0145-2126(91)90061-w
103  Ichiki AT, Sugantharaj DG, Hudson CS, Gerard DA, Barnhill MA, Bamberger EG, Wust CJ, Fuhr JE, Lozzio CB.  Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis  Nat Immun Cell Growth Regul  1988  7(5-6):297-304  PubMed ID: 3146698  
106  Walter H, Krob EJ, Al-Romaihi FA, Johnson D, Lozzio CB.  Detection of surface differences between closely related cell populations by partitioning. Cultured K-562 cell sublines  Cell Biophys  1988  13(3):173-87  PubMed ID: 2465826   DOI: 10.1007/BF02918374
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
95  Ichiki AT, Bamberger EG, Wust CJ, Lozzio CB.  Diversity of cell surface hematopoietic antigens on K-562 sublines identified with monoclonal antibodies  Leuk Res  1986  10(5):565-74  PubMed ID: 2940421   DOI: 10.1016/0145-2126(86)90092-5
96  Ichiki AT, Wust CJ, Lozzio CB.  Characterization of the Fc(IgG) receptor on the pluripotential leukaemia cell K-562  Clin Exp Immunol  1985  59(1):64-8  PubMed ID: 3971599  
116  Rowley PT, Ohlsson-Wilhelm BM, Wisniewski L, Lozzio CB, Lozzio BB.  K562 human leukemia cell passages differ in embryonic globin gene expression  Leuk Res  1984  8(1):45-54  PubMed ID: 6199625  
100  Lozzio BB, Machado EA, Mitchell J, Lozzio CB, Wust CJ, Golde DW.  Proliferation of human malignant hematopoietic cells in immunodeficient mice: suppression by antibody to pluripotent K-562 leukemia cells involves direct cytolysis and effector cells  Blood  1983  61(6):1045-53  PubMed ID: 6132631  
94  Wust CJ, Green M, Lozzio CB, Lozzio BB.  Antibody-dependent cytolysis of the human leukemia cell line K-562 in the absence of effector cells or complement  Blood  1982  59(1):133-40  PubMed ID: 7053760  
102  Ichiki AT, Lozzio BB, Wust CJ, Lozzio CB.  Relationship between the Fc receptor for IgG and the specific associated antigen of the pluripotential leukaemia cell line, K-562  Clin Exp Immunol  1982  50(2):360-5  PubMed ID: 6185259  
105  Machado EA, Gerard DA, Mitchell JR, Lozzio BB, Lozzio CB.  Arrest and extravasation of neoplastic cells. An electron microscopy study of serial sections at sequential stages  Virchows Arch A Pathol Anat Histol  1982  396(1):73-89  PubMed ID: 7123846   DOI: 10.1007/BF00428501
107  Lozzio CB, Lozzio BB, Wust CJ, Bamberger EG, Golde DW, Minowada J.  Antibody lysis of human hematopoietic cells in the absence of complement and effector cells  Exp Hematol  1982  10(8):698-707  PubMed ID: 6183138  
115  Perez RL, Mitchell JR, Lozzio BB.  Relevance of lipids to heterotransplantation of human malignancies  Oncology  1982  39(3):179-84  PubMed ID: 7043353   DOI: 10.1159/000225632
87  Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.  A multipotential leukemia cell line (K-562) of human origin  Proc Soc Exp Biol Med  1981  166(4):546-50  PubMed ID: 7194480   DOI: 10.3181/00379727-166-41106
93  Fuhr JE, Bamberger EG, Lozzio CB, Lozzio BB.  Induction of hemoglobin synthesis in original K 562 cell line  Blood Cells  1981  7(2):389-99  PubMed ID: 6170369  
108  Latif ZA, Lozzio BB, Wust CJ, Krauss S, Aggio MC, Lozzio CB.  Evaluation of drug-antibody conjugates in the treatment of human myelosarcomas transplanted in nude mice  Cancer  1980  45(6):1326-33  PubMed ID: 6928397   DOI: 10.1002/1097-0142(19800315)45:6<1326::aid-cncr2820450610>;2-y
113  Lair SV, Lozzio BB.  Thymocyte reconstitution of athymic and athymic-asplenic mice: graft rejection and antibody synthesis  Exp Cell Biol  1980  48(6):439-44  PubMed ID: 6967835   DOI: 10.1159/000163010
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026   DOI: 10.1016/0145-2126(79)90033-x
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354   DOI: 10.1038/280164a0
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644   DOI: 10.1038/278364a0
89  Lozzio BB, Machado EA, Lair SV, Lozzio CB.  Reproducible metastatic growth of K-562 human myelogenous leukemia cells in nude mice  J Natl Cancer Inst  1979  63(2):295-9  PubMed ID: 287825  
112  Lozzio CB, Lozzio BB, Wust CJ, Kim J.  Correlation of leukemia-associated antigens and Ph1 chromosome in fibroblastlike cells derived from bone marrow  Blood  1978  52(4):673-80  PubMed ID: 99197  
114  Lozzio BB, Lozzio CB, Bamberger EG.  Cytotoxicity of a factor from normal and abnormal human spleens for allogenic leukemia cells  Am J Hematol  1978  4(1):57-65  PubMed ID: 655158   DOI: 10.1002/ajh.2830040108
99  Collins JL, Wust CJ, Lozzio BB, Lozzio CB.  Characterization of an antigen from the myelogenous leukemia cell line K-562  J Natl Cancer Inst  1977  59(6):1667-74  PubMed ID: 72824   DOI: 10.1093/jnci/59.6.1667
109  Lozzio BB, Lozzio CB, Krauss S, Wust CJ, Girardi A.  Leukemia-associated antigens detected by a nonhuman primate antiserum to a Ph1+ myeologenous leukemia cell line  Blood  1977  50(1):115-24  PubMed ID: 68791  
111  Lozzio BB, Machado EA, Lair SA, Lozzio CB.  Suppression of human myelosarcoma growth in athymic mice by a primate antiserum  Cancer Treat Rep  1977  61(9):1679-84  PubMed ID: 271518  
88  Lozzio CB, Lozzio BB, Yang WK, Ichiki AT, Bamberger EG.  Absence of thymus-derived lymphocyte markers in myelogenous leukemia (Ph1+) cell line K-562  Cancer Res  1976  36(12):4657-62  PubMed ID: 63324  
104  Lozzio BB, Lozzi CB, Machado E.  Human myelogenous (Ph+) leukemia cell line: transplantation into athymic mice  J Natl Cancer Inst  1976  56(3):627-9  PubMed ID: 1062625   DOI: 10.1093/jnci/56.3.627
110  Lozzio BB, Machado EA, Lozzio CB, Lair S.  Hereditary asplenic-athymic mice: transplantation of human myelogenous leukemic cells  J Exp Med  1976  143(1):225-31  PubMed ID: 53268   DOI: 10.1084/jem.143.1.225
4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658  

To topTop
User's Publication
21543  Panyajai P, Viriyaadhammaa N, Tima S, Chiampanichayakul S, Dejkriengkraikul P, Okonogi S, Anuchapreeda S.  Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects.  BMC Complement Med Ther  2024  24(1):16  PubMed ID: 38166788   DOI: 10.1186/s12906-023-04261-9
21688  Tsuchiya M, Tachibana N, Hamachi I.  Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo.  Commun Biol  2024  7(1):459  PubMed ID: 38627603   DOI: 10.1038/s42003-024-06164-y
21707  Baba T, Tomaru U, Hirao A, Mukaida N, Johmura Y.  Autophagy Inhibition-induced Cytosolic DNA Sensing Combined with Differentiation Therapy Induces Irreversible Myeloid Differentiation in Leukemia Cells.  Cancer Res Commun  2024  4(3):849-860  PubMed ID: 38466568   DOI: 10.1158/2767-9764.CRC-23-0507
21717  Kasai F, Mizukoshi K, Nakamura Y.  Variable characteristics overlooked in human K-562 leukemia cell lines with a common signature  Sci Rep  2024  14(1):9619  PubMed ID: 38671192   DOI: 10.1038/s41598-024-60271-8
21737  Shiomi A, Kaneko T, Nishikawa K, Tsuchida A, Isoshima T, Sato M, Toyooka K, Doi K, Nishikii H, Shintaku H.  High-throughput mechanical phenotyping and transcriptomics of single cells.  Nat Commun  2024  15(1):3812  PubMed ID: 38760380   DOI: 10.1038/s41467-024-48088-5
18161  Kawatani M, Aono H, Hiranuma S, Shimizu T, Muroi M, Nogawa T, Ohishi T, Ohba SI, Kawada M, Yamazaki K, Dan S, Dohmae N, Osada H.  Identification of a dihydroorotate dehydrogenase inhibitor that inhibits cancer cell growth by proteomic profiling  Oncol Res  2023  31(6):833-844  PubMed ID: 37744270   DOI: 10.32604/or.2023.030241
19542  Tsuchiya M, Tachibana N, Nagao K, Tamura T, Hamachi I.  Organelle-selective click labeling coupled with flow cytometry allows pooled CRISPR screening of genes involved in phosphatidylcholine metabolism  Cell Metab  2023  35(6):1072-1083.e9  PubMed ID: 36917984   DOI: 10.1016/j.cmet.2023.02.014
19668  Lu X, Lofgren SM, Zhao Y, Mazur PK.  Multiplexed transcriptomic profiling of the fate of human CAR T cells in vivo via genetic barcoding with shielded small nucleotides  Nat Biomed Eng  2023  7(9):1170-1187  PubMed ID: 37652986   DOI: 10.1038/s41551-023-01085-3
19765  Ashiba K, Mino K, Okido Y, Sato K, Kawakami H.  Senescence recovering by dual drug-encapsulated liposomal nanoparticles for large-scale human cell expansion  J Artif Organs  2023  26(3):246-250  PubMed ID: 35984542   DOI: 10.1007/s10047-022-01356-x
19942  Kondo S, Ferdousi F, Zhao J, Suidasari S, Yokozawa M, Yamauchi K, Tominaga KI, Isoda H.  Hematinic Potential of Olive Leaf Extract: Evidence from an In Vivo Study in Mice and a Pilot Study in Healthy Human Volunteers  Nutrients  2023  15(19):4095  PubMed ID: 37836379   DOI: 10.3390/nu15194095
21051  Hirosaki H, Maeda Y, Takeyoshi M.  Establishment of Cell-Based Assay System for Evaluating Cytotoxic Activity Modulated by the Blockade of PD-1 and PD-L1 Interactions with a Therapeutic Antibody.  Immunol Invest  2023  1-11  PubMed ID: 36731129   DOI: 10.1080/08820139.2023.2174442
21352  Mise-Omata S, Ando M, Srirat T, Nakagawara K, Hayakawa T, Iizuka-Koga M, Nishimasu H, Nureki O, Ito M, Yoshimura A.  SOCS3 deletion in effector T cells confers an anti-tumorigenic role of IL-6 to the pro-tumorigenic cytokine.  Cell Rep  2023  112940  PubMed ID: 37582370   DOI: 10.1016/j.celrep.2023.112940
21375  Tsuchiya M, Tachibana N, Hamachi I.  Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling.  STAR Protoc  2023  4(3):102525  PubMed ID: 37635353   DOI: 10.1016/j.xpro.2023.102525
17569  Hashino T, Matsubara H, Xu J, Tanaka R, Kusagawa E, Ueda Y, Yoshida H, Kataoka T.  PGAM5 interacts with Bcl-rambo and regulates apoptosis and mitophagy  Exp Cell Res  2022  420(1):113342  PubMed ID: 36075447   DOI: 10.1016/j.yexcr.2022.113342
13403  Takeuchi R, Suzuki M, Yasukawa T.  Electrorotation Rates of K562 Cells Accompanied with the Erythroid Differentiation Induced by Sodium Butyrate.  Anal Sci  2021    PubMed ID: 33390466   DOI: 10.2116/analsci.20C017
20082  Yoshimura A, Asahina Y, Chang LY, Angata T, Tanaka H, Kitajima K, Sato C.  Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells.  J Biol Chem  2021  100477  PubMed ID: 33640457   DOI: 10.1016/j.jbc.2021.100477
16204  Kondo S, Ferdousi F, Yamauchi K, Suidasari S, Yokozawa M, Harrabi MM, Tominaga KI, Isoda H.  Comprehensive transcriptome analysis of erythroid differentiation potential of olive leaf in haematopoietic stem cells  J Cell Mol Med  2021  25(15):7229-7243  PubMed ID: 34180123   DOI: 10.1111/jcmm.16752
21368  Soboleva S, Kurita R, Ek F, Åkerstrand H, Silvério-Alves R, Olsson R, Nakamura Y, Miharada K.  Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines  Commun Biol2021  2021  4(1):677  PubMed ID: 34083702   DOI: 10.1038/s42003-021-02202-1
13052  Kawai S, Suzuki M, Arimoto S, Korenaga T, Yasukawa T.  Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.  Analyst  2020    PubMed ID: 32462157   DOI: 10.1039/d0an00100g
13914  Takano M, Higashi M, Baba S, Kawami M, Yumoto R.  Transport of ribavirin in human myelogenous leukemia cell line K562  Pharmazie  2020  75(7):329-334  PubMed ID: 32635975   DOI: 10.1691/ph.2020.0440
14798  Kondo T, Ando M, Nagai N, Tomisato W, Srirat T, Liu B, Mise-Omata S, Ikeda M, Chikuma S, Nishimasu H, Nureki O, Ohmura M, Hayakawa N, Hishiki T, Uchibori R, Ozawa K, Yoshimura A.  The NOTCH-FOXM1 Axis Plays a Key Role in Mitochondrial Biogenesis in the Induction of Human Stem Cell Memory-like CAR-T Cells  Cancer Res  2020  80(3):471-483  PubMed ID: 31767627   DOI: 10.1158/0008-5472.CAN-19-1196
11509  Zhelev Z, Georgieva E, Lazarova D, Semkova S, Aoki I, Gulubova M, Higashi T, Bakalova R.  Redox Imaging to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers.  Oxid Med Cell Longev  2019    PubMed ID: 31089411   DOI: 10.1155/2019/6373685
11537  Miura S, Kobune M, Horiguchi H, Kikuchi S, Iyama S, Murase K, Goto A, Ikeda H, Takada K, Miyanishi K, Kato J.  EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cells.  Blood Cells Mol. Dis.  2019    PubMed ID: 31082798   DOI: 10.1016/j.bcmd.2019.04.014
10946  Tsubasa Koga, Keiko Morotomi-Yano, Takashi Sakugawa, Hisato Saitoh, Ken-Ichi Yano  Nanosecond pulsed electric fields induce extracellular release of chromosomal DNA and histone citrullination in neutrophil-differentiated HL-60 cells  Sci Rep  2019  9(1):8451  PubMed ID: 31186478   DOI: 10.1038/s41598-019-44817-9
11677  Ryohichi Sugimura, Ryo Ohta, Chihiro Mori, Emi Sano, Tatsuki Sugiyama, Takashi Nagasawa, Akira Niwa,  Angiocrine factors from HUVECs amplify erythroid cells  bioRxiv  2019      DOI: 10.1101/837823
11093  Abdelmoez MN, Iida K, Oguchi Y, Nishikii H, Yokokawa R, Kotera H, Uemura S, Santiago JG, Shintaku H.  SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.  Genome Biol.  2018  19(1):66  PubMed ID: 29871653   DOI: 10.1186/s13059-018-1446-9
4267  Kikuchi G, Kurita R, Ogasawara K, Isa K, Tsuneyama H, Nakamura Y, Yabe R, Shiba M, Tadokoro K, Nagai T, Satake M.  Application of immortalized human erythroid progenitor cell line in serologic tests to detect red blood cell alloantibodies.  Transfusion  2018    PubMed ID: 30180269   DOI: 10.1111/trf.14840
4270  Ichii M, Oritani K, Murase M, Komatsu K, Yamazaki M, Kyoden R, Kito N, Nozaki Y, Saito M, Iwamura H, Kanakura Y.  Molecular targeting of inosine-5'-monophosphate dehydrogenase by FF-10501 promotes erythropoiesis via ROS/MAPK pathway.  Leuk. Lymphoma  2018    PubMed ID: 28730859   DOI: 10.1080/10428194.2017.1339878
4378  Miyauchi M, Koya J, Arai S, Yamazaki S, Honda A, Kataoka K, Yoshimi A, Taoka K, Kumano K, Kurokawa M.  ADAM8 Is an Antigen of Tyrosine Kinase Inhibitor-Resistant Chronic Myeloid Leukemia Cells Identified by Patient-Derived Induced Pluripotent Stem Cells.  Stem Cell Reports  2018  10(3):1115-1130  PubMed ID: 29429960   DOI: 10.1016/j.stemcr.2018.01.015
15049  Ureshino H, Shindo T, Kojima H, Kusunoki Y, Miyazaki Y, Tanaka H, Saji H, Kawaguchi A, Kimura S.  Allelic Polymorphisms of KIR s and HLA s Predict Favorable Responses to Tyrosine Kinase Inhibitors in CML  Cancer Immunol Res  2018  6(6):745-754  PubMed ID: 29695383   DOI: 10.1158/2326-6066.CIR-17-0462
14689  Takagishi T, Takehara M, Seike S, Miyamoto K, Kobayashi K, Nagahama M.  Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation  Sci Rep  2017  7(1):5217  PubMed ID: 28701754   DOI: 10.1038/s41598-017-05567-8
14775  Mahmoud N. Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, Hirofumi Shintaku  Correlation of gene expressions between nucleus and cytoplasm reflects single-cell physiology  bioRxiv  2017  October 20, 2017    DOI: 10.1101/206672
9546  Nakamura A, Monzen S, Takasugi Y, Wojcik A, Mariya Y.  Application of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay.  J. Radiat. Res.  2016  57:121-6  PubMed ID: 26826197   DOI: 10.1093/jrr/rrv103
5654  Sadahira Y, Sugihara T, Fujiwara H, Nishimura H, Suetsugu Y, Takeshita M, Okamura S, Goto M.  WRN protein as a novel erythroblast immunohistochemical marker with applications for the diagnosis of Werner syndrome.  Virchows Arch  2015  466(3):343-50  PubMed ID: 25503078   DOI: 10.1007/s00428-014-1703-6
17441  Shimizu K, Shinga J, Yamasaki S, Kawamura M, Dörrie J, Schaft N, Sato Y, Iyoda T, Fujii S.  Transfer of mRNA Encoding Invariant NKT Cell Receptors Imparts Glycolipid Specific Responses to T Cells and γδT Cells  PLoS One  2015  10(6):e0131477  PubMed ID: 26121617   DOI: 10.1371/journal.pone.0131477
13879  Isoda H, Motojima H, Onaga S, Samet I, Villareal MO, Han J.  Analysis of the erythroid differentiation effect of flavonoid apigenin on K562 human chronic leukemia cells  Chem Biol Interact  2014  220:269-77  PubMed ID: 25058688   DOI: 10.1016/j.cbi.2014.07.006
9087  Kenta Okamoto, Hitomi Kinoshita, Maria Del Carmen Parquet, Muhareva Raekiansyah, Daisuke Kimura, Katsuyuki Yui, Mohammed Alimul Islam, Futoshi Hasebe, Kouichi Morita  Dengue Virus Strain DEN2 16681 Utilizes a Specific Glycochain of syndecan-2 Proteoglycan as a Receptor  J Gen Virol  2012  93 (Pt 4), 761-770  PubMed ID: 22170634   DOI: 10.1099/vir.0.037853-0
9088  Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Hitomi Kurokawa, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato  A Novel PAN/apple Domain-Containing Protein From Toxoplasma Gondii: Characterization and Receptor Identification  PLoS One  2012  7 (1), e30169  PubMed ID: 22276154   DOI: 10.1371/journal.pone.0030169
10993  Soninkhishig Tsolmon, Eri Nakazaki, Junkyu Han, Hiroko Isoda  Apigetrin induces erythroid differentiation of human leukemia cells K562: proteomics approach  Mol Nutr Food Res  2011  55 Suppl 1:S93-S102  PubMed ID: 21480518   DOI: 10.1002/mnfr.201000650
12292  Yujiro Tanaka, Koji Kawahashi, Zen-Ichiro Katagiri, Yasuhiro Nakayama, Milind Mahajan, Dimitris Kioussis  Dual function of histone H3 lysine 36 methyltransferase ASH1 in regulation of Hox gene expression  PLoS One  2011  6(11):e28171  PubMed ID: 22140534   DOI: 10.1371/journal.pone.0028171
18575  Nozaki H, Yanagida M, Koide K, Shiotani K, Kinoshita M, Kobayashi Y, Watarai S, Nakamura K, Suzuki A, Ariga T, Kushi Y.  Production and characterization of monoclonal antibodies specific to lactotriaosylceramide  Glycobiology  2010  20(12):1631-42  PubMed ID: 20693232   DOI: 10.1093/glycob/cwq117
18881  Soninkhishig Tsolmon, Parida Yamada, Hiroko Isoda  Effect of Mongolian Medicinal Plant Stellera Chamaejasme on Chronic Leukemia Cells K562  Basic and Applied Aspects  2010  277-280    DOI: 10.1007/978-90-481-3892-0_45
6125  Shimahara A, Yamakawa N, Nishikata I, Morishita K.  Acetylation of lysine 564 adjacent to the C-terminal binding protein-binding motif in EVI1 is crucial for transcriptional activation of GATA2.  J Biol Chem  2010  285(22):16967-77  PubMed ID: 20363750   DOI: 10.1074/jbc.M110.102046
9155  Soninkhishig Tsolmon, Junkyu Han, Hiroko Isoda  Inhibition of Cell Growth by Stellera Chamaejasme Extract Is Associated With Induction of Autophagy and Differentiation in Chronic Leukemia K562 Cells  J Biosci Bioeng  2010  110 (2), 262-8  PubMed ID: 20547324   DOI: 10.1016/j.jbiosc.2010.02.006
18822  Kondo N, Nishimura S.  MALDI-TOF mass-spectrometry-based versatile method for the characterization of protein kinases  Chemistry  2009  5(6):1413-21  PubMed ID: 19115309   DOI: 10.1002/chem.200801650
5396  Hosomi O, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamakura F, Kudo S.  Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.  Glycoconj J  2009  26(2):189-98  PubMed ID: 18726690   DOI: 10.1007/s10719-008-9175-z
8795  Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761   DOI: 10.1007/978-1-59745-294-6_19
18908  Harada H, Murakami T, Tea SS, Takeuchi A, Koga T, Okada S, Suico MA, Shuto T, Kai H.  Heat shock suppresses human NK cell cytotoxicity via regulation of perforin  Int J Hyperthermia  2007  23(8):657-65  PubMed ID: 18097852   DOI: 10.1080/02656730701822087
3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080   DOI: 10.1152/ajpendo.00502.2004
9051  Tominaga K, Kagata T, Johmura Y, Hishida T, Nishizuka M, Imagawa M.  SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc.  FEBS J.  2005  272:1590-9  PubMed ID: 15794747   DOI: 10.1111/j.1742-4658.2005.04580.x
3170  Harada H, Suzu S, Ito T, Okada S.  Selective expansion and engraftment of human CD16+ NK cells in NOD/SCID mice.  Eur J Immunol  2005  35(12):3599-609  PubMed ID: 16304638   DOI: 10.1002/eji.200535125
9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112   DOI: 10.1021/jf0506129
2706  Harada H, Watanabe S, Saijo K, Ishiwata I, Ohno T  A Wilms tumor cell line, HFWT, can greatly stimulate proliferation of CD56+ human natural killer cells and their novel precursors in blood mononuclear cells.  Exp Hematol  2004  32:614-21  PubMed ID: 15246157   DOI: 10.1016/j.exphem.2004.03.011
2848  Feng, Xingmin, Chuhjo, Tatsuya, Sugimori, Chiharu, Kotani, Takeharu, Lu, Xuzhang, Takami, Akiyoshi, Takamatsu, Hiroyuki, Yamazaki, Hirohito, Nakao, Shinji  Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells.  Blood  2004  104:2425-31  PubMed ID: 15217832   DOI: 10.1182/blood-2004-05-1839
2880  Okada M, Adachi S, Imai T, Watanabe K, Toyokuni SY, Ueno M, Zervos AS, Kroemer G, Nakahata T.  A novel mechanism for imatinib mesylate-induced cell death of BCR-ABL-positive human leukemic cells: caspase-independent, necrosis-like programmed cell death mediated by serine protease activity.  Blood  2004  103(6):2299-307  PubMed ID: 14645012   DOI: 10.1182/blood-2003-05-1605
2983  Kawano T, Horiguchi-Yamada J, Iwase S, Akiyama M, Furukawa Y, Kan Y, Yamada H.  Depsipeptide enhances imatinib mesylate-induced apoptosis of Bcr-Abl-positive cells and ectopic expression of cyclin D1, c-Myc or active MEK abrogates this effect.  Anticancer Res  2004  24(5A):2705-12  PubMed ID: 15517875  
2984  Kawano T, Horiguchi-Yamada J, Saito S, Iwase S, Furukawa Y, Kano Y, Yamada H.  Ectopic cyclin D1 expression blocks STI571-induced erythroid differentiation of K562 cells.  Leuk Res  2004  28(6):623-9  PubMed ID: 15120940   DOI: 10.1016/j.leukres.2003.10.022
2985  Kawano T, Horiguchi-Yamada J, Iwase S, Furukawa Y, Kano Y, Yamada H.  Inactivation of ERK accelerates erythroid differentiation of K562 cells induced by herbimycin A and STI571 while activation of MEK1 interferes with it.  Mol Cell Biochem  2004  58(1-2):25-33  PubMed ID: 15030167   DOI: 10.1023/b:mcbi.0000012830.96393.b9
3118  Uchiyama S, Kobayashi S, Takata H, Ishihara T, Sone T, Matsunaga S, Fukui K.  Protein composition of human metaphase chromosomes analyzed by two-dimensional electrophoreses.  Cytogenet Genome Res  2004  107(1-2):49-54  PubMed ID: 15305056   DOI: 10.1159/000079571
9534  Shunji Aoki, Dexin Kong, Kouhei Matsui, Rachmaniar Rachmat, Motomasa Kobayashi  Sesquiterpene Aminoquinones, From a Marine Sponge, Induce Erythroid Differentiation in Human Chronic Myelogenous Leukemia, K562 Cells  Chem Pharm Bull (Tokyo)   2004  52(8):935-7  PubMed ID: 15304984   DOI: 10.1248/cpb.52.935
9559  Shunji Aoki, Dexin Kong, Kouhei Matsui, Motomasa Kobayashi  Erythroid Differentiation in K562 Chronic Myelogenous Cells Induced by Crambescidin 800, a Pentacyclic Guanidine Alkaloid  Anticancer Res  2004  24(4):2325-30.  PubMed ID: 15330179  
19098  Hagihara M, Tsuchiya T, Hyodo O, Ueda Y, Tazume K, Gansuvd B, Kato S, Hotta T.  The in vitro generation of Ph1+ ALL-specific HLA-A24-restricted cytotoxic T lymphocytes using a synthetic 16 mer minor bcr-abl peptide  Leuk Res  2003  27(3):253-7  PubMed ID: 12537978   DOI: 10.1016/s0145-2126(02)00092-9
3979  Matsumoto K, Akao Y, Kobayashi E, Ohguchi K, Ito T, Tanaka T, Iinuma M, Nozawa Y.  Induction of apoptosis by xanthones from mangosteen in human leukemia cell lines.  J Nat Prod  2003  66(8):1124-7  PubMed ID: 12932141   DOI: 10.1021/np020546u
9586  Kazuko Yamazaki, Emiko Yamada, Yoshio Kanaji, Tetsuo Yanagisawa, Yoshiyuki Kato, Kanji Sato, Kazue Takano, Yuji Sakasegawa, Kiyotoshi Kaneko  Stimulation of Cellular Prion Protein Expression by TSH in Human Thyrocytes  Biochem Biophys Res Commun  2003  305(4):1034-9  PubMed ID: 12767934   DOI: 10.1016/s0006-291x(03)00801-5
13868  Kobayashi T, Yamanaka R, Homma J, Tsuchiya N, Yajima N, Yoshida S, Tanaka R.  Tumor mRNA-loaded dendritic cells elicit tumor-specific CD8(+) cytotoxic T cells in patients with malignant glioma  Cancer Immunol Immunother  2003  52(10):632-7  PubMed ID: 12827308   DOI: 10.1007/s00262-003-0408-5
14421  Takahashi T, Haraguchi K, Chiba S, Yasukawa M, Shibata Y, Hirai H.  Valpha24+ natural killer T-cell responses against T-acute lymphoblastic leukaemia cells: implications for immunotherapy  Br J Haematol  2003  122(2):231-9  PubMed ID: 12846891   DOI: 10.1046/j.1365-2141.2003.04429.x
14422  Ueda Y, Hagihara M, Gansuvd B, Yu Y, Masui A, Okamoto A, Higuchi A, Tazume K, Kato S, Hotta T.  The effects of alphaGalCer-induced TCRValpha24 Vbeta11(+) natural killer T cells on NK cell cytotoxicity in umbilical cord blood  Cancer Immunol Immunother  2003  52(10):625-31  PubMed ID: 12802518   DOI: 10.1007/s00262-003-0398-3
2549  Nagai, Fusako, Hiyoshi, Yasuko, Sugimachi, Kumiko, Tamura, Hiro-Omi  Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines.  Biol Pharm Bull  2002  25:383-5  PubMed ID: 11913539   DOI: 10.1248/bpb.25.383
4885  Sonoyama J, Matsumura I, Ezoe S, Satoh Y, Zhang X, Kataoka Y, Takai E, Mizuki M, Machii T, Wakao H, Kanakura Y.  Functional cooperation among Ras, STAT5, and phosphatidylinositol 3-kinase is required for full oncogenic activities of BCR/ABL in K562 cells.  J Biol Chem  2002  277(10):8076-82  PubMed ID: 11779872   DOI: 10.1074/jbc.M111501200
2979  Horiguchi-Yamada J, Fukumi S, Saito S, Nakayama R, Iwase S, Yamada H.  DNA topoisomerase II inhibitor, etoposide, induces p21WAF1/CIP1 through down-regulation of c-Myc in K562 cells.  Anticancer Res  2002  22(6C):3827-32  PubMed ID: 12553001  
13865  Jiang B, Hattori N, Liu B, Kitagawa K, Inagaki C.  Expression of swelling- and/or pH-regulated chloride channels (ClC-2, 3, 4 and 5) in human leukemic and normal immune cells  Life Sci  2002  70(12):1383-94  PubMed ID: 11883714   DOI: 10.1016/s0024-3205(01)01517-x
15668  Akiyama M, Yamada O, Kanda N, Akita S, Kawano T, Ohno T, Mizoguchi H, Eto Y, Anderson KC, Yamada H.  Telomerase overexpression in K562 leukemia cells protects against apoptosis by serum deprivation and double-stranded DNA break inducing agents, but not against DNA synthesis inhibitors  Cancer Lett  2002  178(2):187-97  PubMed ID: 11867204   DOI: 10.1016/s0304-3835(01)00838-2
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959   DOI: 10.1248/bpb.24.1258
18102  Kawasaki A, Matsumura I, Miyagawa Ji, Ezoe S, Tanaka H, Terada Y, Tatsuka M, Machii T, Miyazaki H, Furukawa Y, Kanakura Y.  Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells  J Cell Biol  2001  152(2):275-87  PubMed ID: 11266445   DOI: 10.1083/jcb.152.2.275
13866  Hattori N, Saito T, Yagyu T, Jiang BH, Kitagawa K, Inagaki C.  GH, GH receptor, GH secretagogue receptor, and ghrelin expression in human T cells, B cells, and neutrophils  J Clin Endocrinol Metab  2001  86(9):4284-91  PubMed ID: 11549663   DOI: 10.1210/jcem.86.9.7866
13876  Aoki S, Higuchi K, Isozumi N, Matsui K, Miyamoto Y, Itoh N, Tanaka K, Kobayashi M.  Differentiation in chronic myelogenous leukemia cell K562 by spongean sesterterpene  Biochem Biophys Res Commun  2001  282(2):426-31  PubMed ID: 11401476   DOI: 10.1006/bbrc.2001.4588
14425  Hagihara M, Tsuchiya T, Ueda Y, Masui A, Gansuvd B, Munkhbat B, Inoue H, Hyodo O, Ando K, Kato S, Hotta T.  Successful in vitro generation of Epstein-Barr virus-specific cytotoxic T lymphocytes from severe chronic active EBV patients  Med Microbiol Immunol  2001  189(3):137-45  PubMed ID: 11388611   DOI: 10.1007/s430-001-8020-z
2986  Fukumi S, Horiguchi-Yamada J, Iwase S, Ohno T, Yamada H.  Concentration-dependent variable effects of etoposide on the cell cycle of CML cells.  Anticancer Res  2000  20(5A):3105-10  PubMed ID: 11062729  
2987  Akiyama M, Yamada O, Akita S, Urashima M, Horiguchi-Yamada J, Ohno T, Mizoguchi H, Eto Y, Yamada H.  Ectopic expression of c-myc fails to overcome downregulation of telomerase activity induced by herbimycin A, but ectopic hTERT expression overcomes it.  Leukemia  2000  14(7):1260-5  PubMed ID: 10914551   DOI: 10.1038/sj.leu.2401828
2988  Fukumi S, Horiguchi-Yamada J, Nakada S, Nagai M, Ohno T, Yamada H.  Differential responses of Bcl-2 family genes to etoposide in chronic myeloid leukemia K562 cells.  Mol Cell Biochem  2000  206(1-2):43-50  PubMed ID: 10839193   DOI: 10.1023/a:1007056727876
15583  Naito K, Takeshita A, Shigeno K, Nakamura S, Fujisawa S, Shinjo K, Yoshida H, Ohnishi K, Mori M, Terakawa S, Ohno R.  Calicheamicin-conjugated humanized anti-CD33 monoclonal antibody (gemtuzumab zogamicin, CMA-676) shows cytocidal effect on CD33-positive leukemia cell lines, but is inactive on P-glycoprotein-expressing sublines  Leukemia  2000  14(8):1436-43  PubMed ID: 10942240   DOI: 10.1038/sj.leu.2401851
2381  Suzuki K, Tsujitani S, Konishi I, Yamaguchi Y, Hirooka Y, Kaibara N.  Expression of MAGE genes and survival in patients with hepatocellular carcinoma.  Int J Oncol  1999  15(6):1227-32  PubMed ID: 10568832   DOI: 10.3892/ijo.15.6.1227
15584  Sekine K, Fujii H, Abe F.  Induction of apoptosis by bestatin (ubenimex) in human leukemic cell lines  Leukemia  1999  13(5):729-34  PubMed ID: 10374877   DOI: 10.1038/sj.leu.2401388
2500  Gonda K, Okamoto H, Takuwa N, Yatomi Y, Okazaki H, Sakurai T, Kimura S, Sillard R, Harii K, Takuwa Y.  The novel sphingosine 1-phosphate receptor AGR16 is coupled via pertussis toxin-sensitive and -insensitive G-proteins to multiple signalling pathways  Biochem J  1999  337 ( Pt 1)(Pt 1):67-75  PubMed ID: 9854026  
2991  Yamada H, Horiguchi-Yamada J, Nagai M, Takahara S, Sekikawa T, Kawano T, Itoh K, Fukumi S, Iwase S.  Biological effects of a relatively low concentration of 1-beta-D-arabinofuranosylcytosine in K562 cells: alterations of the cell cycle, erythroid-differentiation, and apoptosis.  Mol Cell Biochem  1998  187(1-2):211-20  PubMed ID: 9788759   DOI: 10.1023/a:1006874931249
2320  Kim C, Matsumura M, Saijo K, Ohno T.  In vitro induction of HLA-A2402-restricted and carcinoembryonic-antigen-specific cytotoxic T lymphocytes on fixed autologous peripheral blood cells.  Cancer Immunol Immunother.  1998  47(2):90-6  PubMed ID: 9769117   DOI: 10.1007/s002620050508
2318  Liu SQ, Kawai K, Shiraiwa H, Hayashi H, Akaza H, Hashizaki K, Shiba R, Saijo K, Ohno T.  High rate of induction of human autologous cytotoxic T lymphocytes against renal carcinoma cells cultured with an interleukin cocktail.  Jpn J Cancer Res  1998  89(11):1195-201  PubMed ID: 9914789   DOI: 10.1111/j.1349-7006.1998.tb00515.x
15667  Chino M, Nishikawa K, Yamada A, Ohsono M, Sawa T, Hanaoka F, Ishizuka M, Takeuchi T.  Effect of a novel antibiotic, heliquinomycin, on DNA helicase and cell growth  J Antibiot (Tokyo)  1998  ;51(5):480-6  PubMed ID: 9666176   DOI: 10.7164/antibiotics.51.480
18052  Kamio E, Sakamoto Y, Kimura T, Takakuwa H, Sakurai M, Hosokawa JI, Nakagawa K, Yoshida F, Tagami K.  Increased natural killer resistance to cyclosporine A by continuous doses of dexamethasone in rats  Immunology  1997  92(3):407-11  PubMed ID: 9486116   DOI: 10.1046/j.1365-2567.1997.00363.x
18070  Komata T, Tanaka R, Yamamoto K, Oda T, Ono K, Yoshida S, Takahashi M.  B7-1(CD80)-transfected human glioma cells and interleukin-12 directly stimulate allogeneic CD8+ T cells  J Immunother  1997  20(4):256-64  PubMed ID: 9220315   DOI: 10.1097/00002371-199707000-00002
13863  Isoda H, Nakahara T.  Mannosylerythritol lipid induces granulocytic differentiation and inhibits the tyrosine phosphorylation of human myelogenous leukemia cell line K562  Cytotechnology  1997  25(1-3):191-5  PubMed ID: 22358891   DOI: 10.1023/A:1007982909932
2054  Enomoto K, Furuya K, Moore, R C, Yamagishi S, Oka T, Maeno T  Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells  Biological Signals  1996  5:9-21  PubMed ID: 8739319   DOI: 10.1159/000109169
18001  Shibata Y, Takiguchi H, Tamura K, Yamanaka K, Tezuka M, Abiko Y.  Stimulation of interleukin-1beta-converting enzyme activity during growth inhibition by CPT-11 in the human myeloid leukemia cell line K562  Biochem Mol Med  1996  57(1):25-30  PubMed ID: 8812723   DOI: 10.1006/bmme.1996.0005
2989  Yamada H, Iwase S, Nagai M, Nemoto T, Sekikawa T, Takahara S, Nakada S, Furukawa Y, Horiguchi-Yamada J.  Herbimycin A down-regulates messages of cyclin D1 and c-myc during erythroid differentiation of K562 cells.  Int J Hematol  1996  65(1):31-40  PubMed ID: 8990623   DOI: 10.1016/s0925-5710(96)00526-9
13880  Okada M, Sagawa T, Tominaga A, Kodama T, Hitsumoto Y.  Two mechanisms for platelet-mediated killing of tumour cells: one cyclo-oxygenase dependent and the other nitric oxide dependent  Immunology  1996  89(1):158-64  PubMed ID: 8911154   DOI: 10.1046/j.1365-2567.1996.d01-716.x
1910  Liu SQ, Saijo K, Todoroki T, Ohno T.  Induction of human autologous cytotoxic T lymphocytes on formalin-fixed and paraffin-embedded tumour sections  Nat Med  1995  1(3):267-71  PubMed ID: 7585045   DOI: 10.1038/nm0395-267
13867  Hayakawa T, Yamashita K, Ohuchi E, Shinagawa A.  Cell growth-promoting activity of tissue inhibitor of metalloproteinases-2 (TIMP-2)  J Cell Sci  1994  107 ( Pt 9):2373-9.  PubMed ID: 7844157   DOI: 10.1242/jcs.107.9.2373
15581  Kojima N, Saito M, Tsuji S.  Role of cell surface O-linked oligosaccharides in adhesion of HL60 cells to fibronectin: regulation of integrin-dependent cell adhesion by O-linked oligosaccharide elongation  Exp Cell Res  1994  214(2):537-42  PubMed ID: 7925647   DOI: 10.1006/excr.1994.1291
1901  Liu SQ, Shiba R, Kim BS, Saijo K, Ohno T.  Long-term serum/plasma-free culture of human cytotoxic T lymphocytes i  Cancer Immunol Immunother  1994  39(5):279-85  PubMed ID: 7987858   DOI: 10.1007/BF01519979
17908  Ohmori H, Ikeda H, Tanigawa T, Takai T, Hikida M.  Enzyme release assay of human NK cell activity using beta-galactosidase-expressing K562 target cell line  J Immunol Methods  1993  164(1):131-5  PubMed ID: 8360503   DOI: 10.1016/0022-1759(93)90283-d
1332  Shionoya S.  Etoposide inhibition of methylaminoisobutyric acid transport and its recovery in K562 cells.  Leuk Res  1991  15(2-3):137-41  PubMed ID: 2016905   DOI: 10.1016/0145-2126(91)90094-a

Back Back Return Top Page