Cell No. : Cell Name
RCB1635 : K-562
update : 2021/02/26
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| Comment | Human CML derived cell line. Deposited directly from the institution that has established this cell. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Ichiki, T. Albert
|
| Originator |
Lozzio, C.B. & Lozzio, B.B.
|
| Year of deposit |
2000
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
53 years
|
| Tissue |
pleural effusion
|
| Disease name |
chrinic myelogenous leukemia
|
| Classification |
cancer
|
| History |
overseas
|
|
Lifespan |
infinite
|
| Morphology |
lymphoblast-like
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Suspension cells
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| Medium and additives |
|
MEM + 15% FBS + 0.1mM NEAA
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
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1.5 x 10^(4) cells/ml
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| Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 5% Glycerol
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
|
| STR(human) |
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OK
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| Virus (HIV) |
|
LT 400
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
62-67(50) : /63(3),64(8),65(33),66(4),67(1)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
1
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