Cell No. : Cell Name
RCB1635 : K-562
update : 2021/02/26
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Comment | Human CML derived cell line. Deposited directly from the institution that has established this cell. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Ichiki, T. Albert
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Originator |
Lozzio, C.B. & Lozzio, B.B.
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Year of deposit |
2000
|
Animal |
human
< Mammals
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Genus |
Homo
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Species |
sapiens
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Gender |
Female
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Age at sampling |
53 years
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Tissue |
pleural effusion
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Disease name |
chrinic myelogenous leukemia
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Classification |
cancer
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History |
overseas
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|
Lifespan |
infinite
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Morphology |
lymphoblast-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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Medium and additives |
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MEM + 15% FBS + 0.1mM NEAA
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Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage cell No |
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1.5 x 10^(4) cells/ml
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Passage ratio |
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1 : 10 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 5% Glycerol
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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STR(human) |
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OK
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Virus (HIV) |
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LT 400
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Virus (HTLV-1) |
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(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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62-67(50) : /63(3),64(8),65(33),66(4),67(1)
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Images |
deposit info | lot info |
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Reference information |
Reference |
3
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User's Publication |
1
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