Cell No. : Cell Name
RCB5983 : sHUDEP-2
update : 2024/11/19
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| Comment | |
| Comment from the depositor | A derivative cell line of HUDEP-2 (RCB4557) with the genetic mutation in β globin that causes sickle cell anemia. |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, the following two papers must be cited. PLoS One 8(3): e59890 (2013). Molecular Therapy Methods & Clinical Development 17: 912-921 (2020). 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, the following acknowledgement must be expressed. “The sHUDEP-2 cells were generated by Dr. John F. Tisdale of the National Heart, Lung, and Blood Institute as a derivative of the HUDEP-2 cell line originally obtained from RIKEN BRC.” |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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IPR |
National Heart, Lung, and Blood Institute (USA) & RIKEN BioResource Research Center (Japan)
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| Depositor |
John Tisdale
|
| Year of deposit |
2023
|
| Original cell |
HUDEP-2 (RCB4457)
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Tissue |
umbilical cord blood
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| Classification |
transformed
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| Recombinant |
recombinant
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| Genome Editing |
The genetic mutation in β globin that causes sickle cell anemia was introduced into the parental cell line, HUDEP-2.
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| Exogene |
KUSABIRA-ORANGE, HPV16 E6/E7
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| Vector |
CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml)
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StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10 -6M Dexamethasone + 1ug/ml Doxycycline
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 4-8 split
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| SC frequency |
Subculture : 2 times/week
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Subculture : 2 times/week
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| Temperature |
37
℃
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37
℃
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| CO2 concentration |
5
%
|
5
%
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| Freeze medium |
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
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(-)
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| STR(human) |
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OK
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
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18156
Demirci S, Gudmundsdottir B, Li Q, Haro-Mora JJ, Nassehi T, Drysdale C, Yapundich M, Gamer J, Seifuddin F, Tisdale JF, Uchida N.
βT87Q-Globin Gene Therapy Reduces Sickle Hemoglobin Production, Allowing for Ex Vivo Anti-sickling Activity in Human Erythroid Cells
Mol Ther Methods Clin Dev
2020
17:912-921
PubMed ID: 32405513
DOI: 10.1016/j.omtm.2020.04.013
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6742
Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One
2013
8(3):e59890
PubMed ID: 23533656
DOI: 10.1371/journal.pone.0059890
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