Cell No. : Cell Name
RCB5964 : S-HUDEP2
update : 2024/11/18
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| Comment | |
| Comment from the depositor | A derivative cell line of HUDEP-2 (RCB4557) carrying a biallelic SCD (sickle cell disease) mutation. |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, the following two papers must be cited. PLoS One 8(3): e59890 (2013). Sci Adv.: 8(42): eabo7676(2022). |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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IPR |
William Marsh Rice University (USA) & RIKEN BioResource Research Center (Japan)
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| Depositor |
Gang Bao
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| Year of deposit |
2023
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| Original cell |
HUDEP-2 (RCB4457)
|
| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
|
| Race |
Japanese
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| Tissue |
umbilical cord blood
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| Classification |
transformed
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| Recombinant |
recombinant
|
| Genome Editing |
CRISPR/Cas9 : A biallelic SCD (sickle cell disease) mutation was introduced into the parental cell line, HUDEP-2.
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| Exogene |
KUSABIRA-ORANGE, HPV16 E6/E7
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| Vector |
CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml)
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StemSpan SFEM + 3U/ml EPO + 50ng/ml SCF + 10 -6M Dexamethasone + 1ug/ml Doxycycline
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| Antibiotics |
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Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
Subculture : 2 times/week
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Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
|
CELLBANKER 2
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
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(-)
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| STR(human) |
|
OK
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| Reference information |
Reference |
4
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| User's Publication |
0
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| Reference |
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18194
Park SH, Cao M, Pan Y, Davis TH, Saxena L, Deshmukh H, Fu Y, Treangen T, Sheehan VA, Bao G.
Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing
Sci Adv
2022
8(42):eabo7676
PubMed ID: 36269834
DOI: 10.1126/sciadv.abo7676
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18196
Patino CA, Pathak N, Mukherjee P, Park SH, Bao G, Espinosa HD.
Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering
Sci Adv
2022
8(29):eabn7637.
PubMed ID: 35867793
DOI: 10.1126/sciadv.abn7637
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18192
Alicia Chang, So Hyun Park, Ciaran M Lee, Alireza Paikari, Gang Bao, Vivien A Sheehan
Sickle Human Umbilical Cord Derived Erythroid Progenitor Cells (S-HUDEP2): An Ideal in-Vitro System for Screening Anti-Sickling Compounds for Sickle Cell Disease
Blood
2018
132:3675-3675
DOI: 10.1182/blood-2018-99-117158
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6742
Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
PLoS One
2013
8(3):e59890
PubMed ID: 23533656
DOI: 10.1371/journal.pone.0059890
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