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Cell No. : Cell Name
RCB3683 : HL-60  update : 2019/11/25
CommentHuman cell line derived from promyelocytic leukemia cells. TKG0345 (Deposited from Tohoku Univ.).Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Collins, S. J.
Year of deposit 2011
Original cell TKG0345
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 36 years
Tissue peripheral blood
Case history promyelocytic leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0345)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Reference information Reference 3
User's Publication 23

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1468  Shimizu N, Ohta M, Fujiwara C, Sagara J, Mochizuki N, Oda T, Utiyama H  A Gene Coding for a Zinc Finger Protein Is Induced during 12-O-Tetradecanoylphorbol-13-Acetate-Stimulated HL-60 Cell Differentiation  J Biochem  1992  111:272-277  PubMed ID: 1569051
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
973  Collins, S J, Gallo, R C, Gallagher, R E  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.  Nature  1977  270:347-9  PubMed ID: 271272

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User's Publication
11903  Takishita Y, Yasuda H, Shimizu M, Matsuo A, Morita A, Tsutsumi T, Tsuchiya M, Sato EF.  Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells.  J Clin Biochem Nutr  2020    PubMed ID: 32001952
11984  Wu Y, Zhou Y, Huang CJ, Kobayashi H, Yan S, Ozeki Y, Wu Y, Sun CW, Yasumoto A, Yatomi Y, Lei C, Goda K.  Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging.  Opt Express  2020    PubMed ID: 32118978
12132  Nemidkanam V, Kato Y, Kubota T, Chaichanawongsaroj N.  Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis.  BMC Complement Med Ther  2020    PubMed ID: 32321502
12165  Kiyomi Hikita, Satomi Saigusa, Yuto Takeuchi, Haruka Matsuyama, Rina Nagai, Kuniki Kato, Tomiyasu Murata, Hitoshi Tanaka, Yogesh S.Wagh, Naoki Asao, Norio Kaneda  Induction of enantio-selective apoptosis in human leukemia HL-60 cells by (S)-erypoegin K, an isoflavone isolated from Erythrina poeppigiana  Bioorganic & Medicinal Chemistry  2020    PubMed ID: 32299660
12170  Haochen Yan, Yunzhao Wu, Yuqi Zhou, Muzhen Xu, Petra Paiè,, Cheng Lei, Sheng Yan, and Keisuke Goda  Virtual optofluidic time-stretch quantitative phase imaging  APL Photonics  2020   
11357  Yasuda H, Sonoda A, Yamamoto M, Kawashima Y, Takishita Y, Morita A, Tsutsumi T, Tsuchiya M, Sato EF.  17-β-estradiol enhances neutrophil extracellular trap formation by interaction with estrogen membrane receptor.  Arch. Biochem. Biophys.  2019    PubMed ID: 30590021
11415  Shinada H, Watanabe T, Okudaira K, Iwase Y, Nishi K, Yumita N.  Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells.  Anticancer Res.  2019    PubMed ID: 30711946
11442  Tabei Y, Fukui H, Nishioka A, Hagiwara Y, Sato K, Yoneda T, Koyama T, Horie M.  Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells.  Sci Rep  2019    PubMed ID: 30778158
11619  Mizutani H, Hayashi Y, Hashimoto M, Imai M, Ichimaru Y, Kitamura Y, Ikemura K, Miyazawa D, Ohta K, Ikeda Y, Maeda T, Yoshikawa M, Hiraku Y, Kawanishi S.  Oxidative DNA Damage and Apoptosis Induced by Aclarubicin, an Anthracycline: Role of Hydrogen Peroxide and Copper.  Anticancer Res.  2019    PubMed ID: 31262868
11678  Yoshinori Saito, Mayu Ichihara, Koji Takiguchi, Ayumi Ohsaki, Yasuko Okamoto, Chiaki Kuroda, Xun Gong, Motoo Tori  Bisabolane, Oplopane, and Lignan Constituents of Cremanthodium campanulatum Collected in China  Natural Product Communications  2019   
11807  Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S.  Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells.  Anticancer Res.  2019    PubMed ID: 31519583
11939  Okuda T, Shimizu K, Hasaba S, Date M.  Induction of specific adaptive immune responses by immunization with newly designed artificial glycosphingolipids.  Sci Rep  2019    PubMed ID: 31827147
10616  Hikita K, Hattori N, Takeda A, Yamakage Y, Shibata R, Yamada S, Kato K, Murata T, Tanaka H, Kaneda N.  Potent apoptosis-inducing activity of erypoegin K, an isoflavone isolated from Erythrina poeppigiana, against human leukemia HL-60 cells.  J Nat Med  2018  72:260-266  PubMed ID: 29151157
4228  Shiromizu S, Kusunose N, Matsunaga N, Koyanagi S, Ohdo S.  Optimizing the dosing schedule of l-asparaginase improves its anti-tumor activity in breast tumor-bearing mice.  J. Pharmacol. Sci.  2018    PubMed ID: 29605274
10286  Oyama M, Tokiwano T, Kawaii S, Yoshida Y, Mizuno K, Oh K, Yoshizawa Y.  Protodioscin, Isolated from the Rhizome of Dioscorea tokoro Collected in Northern Japan is the Major Antiproliferative Compound to HL-60 Leukemic Cells.  Curr Bioact Compd  2017  13:170-174  PubMed ID: 28579930
10508  Kitamura H, Ishino T, Shimamoto Y, Okabe J, Miyamoto T, Takahashi E, Miyoshi I.  Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells.  Mediators Inflamm.  2017  2017:6909415  PubMed ID: 29138532
10575  Nakai S, Imaizumi T, Watanabe T, Iwase Y, Nishi K, Okudaira K, Yumita N.  Photodynamically-induced Apoptosis Due to Ultraviolet A in the Presence of Lomefloxacin in Human Promyelocytic Leukemia Cells.  Anticancer Res.  2017  37:6407-6413  PubMed ID: 29061826
9799  Uto T, Tung NH, Thongjankaew P, Lhieochaiphant S, Shoyama Y.  Kayeassamin A Isolated from the Flower of Mammea siamensis Triggers Apoptosis by Activating Caspase-3/-8 in HL-60 Human Leukemia Cells.  Pharmacognosy Res  2016  8:244-248  PubMed ID: 27695262
10914    ANTIPROLIFERATIVE, ANTIOXIDANT ACTIVITIES AND APOPTOSIS INDUCTION BY Morinda lucida AND Taraxacum officinale IN HUMAN HL-60 LEUKEMIA CELLS  Journal of Global Biosciences  2016  7, 4281-4291 
7333  Nishiura H, Yamanegi K, Kawabe M, Kato-Kogoe N, Yamada N, Nakasho K.  The roles of ribosomal protein S19 C-terminus in a shortened neutrophil lifespan through delta lactoferrin.  Immunobiology  2015  220:1085-92  PubMed ID: 26003841
7341  Hattori T, Nakashima K, Marutani T, Kiso Y, Nishi Y, Mukai H.  Successful acquisition of a neutralizing monoclonal antibody against a?novel neutrophil-activating peptide, mitocryptide-1.  Biochem. Biophys. Res. Commun.  2015  463:54-9  PubMed ID: 25986736
7477  Ikeda Y, Yoshinari T, Nagasaki Y.  A novel biointerface that suppresses cell morphological changes by scavenging excess reactive oxygen species.  J Biomed Mater Res A  2015  103:2815-22  PubMed ID: 25691268
7537  K. Tanabe, A. Yamagishi, M. Yokokawa, Y. Morimoto, M. Kinoshita, H. Suzuki  Microdevice for the measurement of neutrophil activity for stress monitoring.  2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)  2015  1707-1710 

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