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Cell No. : Cell Name
RCB1517 : A-6(ES derived)  update : 2022/04/04
CommentMouse ES ( AES0001 H-1 ) cell derived cell, useful for study of hematopoiesis.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Atsumi, Tadao
Originator Atsumi, Tadao
Year of deposit 1999
Animal mouse < Mammals
Strain name C3H
Gender Male
Age at sampling embryo
Tissue ES cell (H-1)
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_N545
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM/HamF12 + 20% FBS + 10μg/ml Insulin + 10μg/ml Transferrin + 0.1mM 2-Mercaptoethanol + 10ng/ml basic FGF
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : every 2 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2678  Anzai H, Ikawa Y, Atsumi T  Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6.  Biochem Biophys Res Commun  2001  282:940-6  PubMed ID: 11352642

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