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Cell No. : Cell Name
AES0001 : H-1  update : 2019/12/26
CommentIn vitro differentiation to various type tissue cells(e.g. neuron,cardiomyocyte,hematopoietic cells)
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
approver's address
The National Agriculture and Food Research Organization
Institute of Agrobiological Sciences, Division of Animal Science
1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
Dr.Kitani, Hiroshi
Fax. +81-29-838-6043
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Atsumi, Tadao
Originator Kitani, Hiroshi
Year of deposit 2001
Another name RCB1778
Animal mouse < Mammals
Strain name C3H
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 15% FBS + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5 x 10^(5) cells/60 mm dish
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells C57BL/6J-emb
Feeder cells No. RCB1312
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 9

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2678  Anzai H, Ikawa Y, Atsumi T  Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6.  Biochem Biophys Res Commun  2001  282:940-6  PubMed ID: 11352642
2564  Anzai H, Nagayoshi M, Obata M, Ikawa Y, Atsumi T  Self-renewal and differentiation of a basic fibroblast growth factor-dependent multipotent hematopoietic cell line derived from embryonic stem cells.  Dev Growth Differ  1999  41:51-8  PubMed ID: 10445502
2562  Kitani H, Takagi N, Atsumi T, Kawakura K, Imamura K, Goto S, Kusakabe M, Fukuta K  Isolation of a germline-transmissible embryonic stem (ES) cell line from C3H/He mice.  Zoolog sci  1996  13:865-871  PubMed ID: 9107142
2563  Atsumi, T. et al    Dev Growth Differ  1995  37:167-172 

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User's Publication
9809  Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada  Identification of RNA Biomarkers for Chemical Safety Screening in Neural Cells Derived From Mouse Embryonic Stem Cells Using RNA Deep Sequencing Analysis  Biochem Biophys Res Commun   2019  512(4):641-646  PubMed ID: 30497775
10612  Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M, Sato H.  Effect of methyl p-hydroxybenzoate on the culture of mammalian cell.  Drug Discov Ther  2017  11:276-280  PubMed ID: 29162775
9608  Tani H, Takeshita J, Aoki H, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M.  Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene.  J. Biosci. Bioeng.  2016  122:329-33  PubMed ID: 26975756
7816  Fujikawa-Yamamoto K, Miyagoshi M, Ota T, Yamagishi H.  Haploid unit-ploidy transition of tetraploid and octaploid H1 (ES) cells in long-term culturing.  Hum. Cell  2011  24:78-85  PubMed ID: 21573878
7828  Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.  Hexaploid H1 (ES) cells established from octaploid H1 cells are as DNA stable as pentaploid H1 cells.  Hum. Cell  2011  24:13-20  PubMed ID: 21547691
7882  Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.  DNA-unstable decaploid mouse H1 (ES) cells established from DNA-stable pentaploid H1 (ES) cells polyploidized using demecolcine.  Cell Prolif.  2011  44:111-9  PubMed ID: 21401752
8026  Luo X, Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.  Different responses between diploid and tetraploid H1 embryonic stem cells appeared during long-term culturing in L15F10 medium without leukemia inhibitory factor.  Hum. Cell  2010  23:134-40  PubMed ID: 21166884
8215  Horie M, Ito A, Kiyohara T, Kawabe Y, Kamihira M.  E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells.  J. Biosci. Bioeng.  2010  110:582-7  PubMed ID: 20587371
8912  Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.  Polyploidization of mouse H-1 (ES) cells by demecolcine and K-252a .  Cytologia  2006  71:399-406 

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