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Cell No. : Cell Name
AES0001 : H-1  update : 2023/04/11
CommentIn vitro differentiation to various type tissue cells(e.g. neuron,cardiomyocyte,hematopoietic cells)
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Atsumi, Tadao
Originator Kitani, Hiroshi
Year of deposit 2001
Another name RCB1778
Animal _mouse < Mammals
Strain name C3H
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_N541
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 15% FBS + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5 x 10 5 cells/60 mm dish
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells C57BL/6J-emb
Feeder cells No. RCB1312
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10 5 cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 14

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2678  Anzai H, Ikawa Y, Atsumi T  Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6.  Biochem Biophys Res Commun  2001  282:940-6  PubMed ID: 11352642   DOI: 10.1006/bbrc.2001.4674
2564  Anzai H, Nagayoshi M, Obata M, Ikawa Y, Atsumi T  Self-renewal and differentiation of a basic fibroblast growth factor-dependent multipotent hematopoietic cell line derived from embryonic stem cells.  Dev Growth Differ  1999  41:51-8  PubMed ID: 10445502   DOI: 10.1046/j.1440-169x.1999.00412.x
2562  Kitani H, Takagi N, Atsumi T, Kawakura K, Imamura K, Goto S, Kusakabe M, Fukuta K  Isolation of a germline-transmissible embryonic stem (ES) cell line from C3H/He mice.  Zoolog sci  1996  13:865-871  PubMed ID: 9107142   DOI: 10.2108/zsj.13.865
2563  Atsumi, T. et al    Dev Growth Differ  1995  37:167-172   

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User's Publication
9809  Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada  Identification of RNA Biomarkers for Chemical Safety Screening in Neural Cells Derived From Mouse Embryonic Stem Cells Using RNA Deep Sequencing Analysis  Biochem Biophys Res Commun   2019  512(4):641-646  PubMed ID: 30497775   DOI: 10.1016/j.bbrc.2018.11.141
10612  Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M, Sato H.  Effect of methyl p-hydroxybenzoate on the culture of mammalian cell.  Drug Discov Ther  2017  11:276-280  PubMed ID: 29162775   DOI: 10.5582/ddt.2017.01054
14691  Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Sato H, Torimura M.  Identification of RNA biomarkers for chemical safety screening in mouse embry  PLoS One  2017  12(7):e0182032  PubMed ID: 28750099   DOI: 10.1371/journal.pone.0182032
9608  Tani H, Takeshita J, Aoki H, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M.  Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene.  J. Biosci. Bioeng.  2016  122:329-33  PubMed ID: 26975756   DOI: 10.1016/j.jbiosc.2016.02.007
15736  Fujikawa-Yamamoto K, Ota T, Miyagoshi M, Yamagishi H.  Pluripotency of a polyploid H1 (ES) cell system without leukaemia inhibitory factor  Cell Prolif  2012  45(2):140-7  PubMed ID: 22288737   DOI: 10.1111/j.1365-2184.2011.00805.x
7816  Fujikawa-Yamamoto K, Miyagoshi M, Ota T, Yamagishi H.  Haploid unit-ploidy transition of tetraploid and octaploid H1 (ES) cells in long-term culturing.  Hum. Cell  2011  24:78-85  PubMed ID: 21573878   DOI: 10.1007/s13577-011-0017-0
7828  Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.  Hexaploid H1 (ES) cells established from octaploid H1 cells are as DNA stable as pentaploid H1 cells.  Hum. Cell  2011  24:13-20  PubMed ID: 21547691   DOI: 10.1007/s13577-010-0003-y
7882  Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.  DNA-unstable decaploid mouse H1 (ES) cells established from DNA-stable pentaploid H1 (ES) cells polyploidized using demecolcine.  Cell Prolif.  2011  44:111-9  PubMed ID: 21401752   DOI: 10.1111/j.1365-2184.2011.00734.x
8026  Luo X, Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.  Different responses between diploid and tetraploid H1 embryonic stem cells appeared during long-term culturing in L15F10 medium without leukemia inhibitory factor.  Hum. Cell  2010  23:134-40  PubMed ID: 21166884   DOI: 10.1111/j.1749-0774.2010.00094.x
8215  Horie M, Ito A, Kiyohara T, Kawabe Y, Kamihira M.  E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells.  J. Biosci. Bioeng.  2010  110:582-7  PubMed ID: 20587371   DOI: 10.1016/j.jbiosc.2010.06.002
14693  Sakai S, Hashimoto I, Kawakami K.  Production of cell-enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules  Biotechnol Bioeng  2008  99(1):235-43  PubMed ID: 17705234   DOI: 10.1002/bit.21624
14694  Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H, Luo X.  Alteration and preservation of cellular characteristics in long-term culture of tetraploid H-1 (ES) cells  Hum Cell  2008  21(2):18-27  PubMed ID: 18397471   DOI: 10.1111/j.1749-0774.2008.00047.x
14692  Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.  Establishment of a tetraploid cell line from mouse H-1 (ES) cells highly polyploidized with demecolcine  Cell Prolif  2007  40(3):327-37  PubMed ID: 17531078   DOI: 10.1111/j.1365-2184.2007.00442.x
8912  Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.  Polyploidization of mouse H-1 (ES) cells by demecolcine and K-252a .  Cytologia  2006  71:399-406    DOI: 10.1508/cytologia.71.399

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