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細胞番号 : 細胞名
RCB3683 : HL-60  update : 2024/01/17
細胞特性(Comment:英)Human cell line derived from promyelocytic leukemia cells. HL60 (RCB0041) can proliferate better than this HL-60 immediately after thawing. TKG0345 (Deposited from Tohoku Univ.). Application consideration
細胞特性(日)ヒト前骨髄球性白血病細胞由来細胞株。 HL60 (RCB0041) の方が、融解直後の増殖性が良好です。 TKG0345 (東北大学医用細胞資源センターからの寄託)。培養開始にあたっての注意
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Fukuda, Hiroshi
樹立者 Collins, S. J.
寄託日 2011
元の細胞 TKG0345
動物種 _human < Mammals
属名 Homo
種名 sapiens
性別 Female
36 years
採取組織 peripheral blood
病名 promyelocytic leukemia
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0345)
細胞寿命 infinite
細胞形態 lymphocyte-like
Cellosaurus(Expasy) CVCL_0002
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Suspension cells
培地 RPMI1640 + 10% FBS
培養方法 浮遊細胞の培養に関する一般的な注意
抗生物質 Free
継代方法 dilution
継代密度 1 : 4-8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
ウィルス(HIV) (-)
ウィルス(HTLV-1) (-)
個体識別検査 OK
文献情報 Reference(英) 6件
Reference(日) 0件
利用者成果(英) 42件
利用者成果(日) 0件

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Reference(英)
146  Huang XJ, Wiernik PH, Klein RS, Gallagher RE.  Arsenic trioxide induces apoptosis of myeloid leukemia cells by activation of caspases  Med Oncol  1999  16(1):58-64  PubMed ID: 10382944   DOI: 10.1007/BF02787360
1468  Shimizu N, Ohta M, Fujiwara C, Sagara J, Mochizuki N, Oda T, Utiyama H  A Gene Coding for a Zinc Finger Protein Is Induced during 12-O-Tetradecanoylphorbol-13-Acetate-Stimulated HL-60 Cell Differentiation  J Biochem  1992  111:272-277  PubMed ID: 1569051   DOI: 10.1093/oxfordjournals.jbchem.a123748
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
145  Gallagher RE, Ferrari AC, Zulich AW, Testa JR.  Variable differentiative response of 6-thioguanine-resistant HL60 sublines: possible relationship to double-minute chromosomes  Prog Nucleic Acid Res Mol Biol  1983  29:283-6  PubMed ID: 6582531  
144  Gallagher R, Collins S, Trujillo J, McCredie K, Ahearn M, Tsai S, Metzgar R, Aulakh G, Ting R, Ruscetti F, Gallo R.  Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia  Blood  1979  54(3):713-33  PubMed ID: 288488  
973  Collins, S J, Gallo, R C, Gallagher, R E  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.  Nature  1977  270:347-9  PubMed ID: 271272   DOI: 10.1038/270347a0

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利用者成果(英)
21600  Das S, Roayapalley PK, Sakagami H, Umemura N, Gorecki DKJ, Hossain M, Kawase M, Das U, Dimmock JR.  Dimeric 3,5-Bis(benzylidene)-4-piperidones: Tumor-Selective Cytotoxicity and Structure-Activity Relationships.  Medicines (Basel)  2024  11(1)  PubMed ID: 38248717   DOI: 10.3390/medicines11010003
21604  Nishimori M, Hayasaka N, Otsui K, Inoue N, Asakura J, Nagao M, Toh R, Ishida T, Hirata KI, Furuyashiki T, Shinohara M.  Stress-induced stenotic vascular remodeling via reduction of plasma omega-3 fatty acid metabolite 4-oxoDHA by noradrenaline.  Sci Rep  2024  14(1):4178  PubMed ID: 38378892   DOI: 10.1038/s41598-024-54867-3
19927  Ishihara T, Tanaka KI, Takafuji A, Miura K, Mizushima T.  Attenuation of LPS-Induced Lung Injury by Benziodarone via Reactive Oxygen Species Reduction  Int J Mol Sci  2023  24(12):10035  PubMed ID: 37373184   DOI: 10.3390/ijms241210035
21091  Yuan B, Kikuchi H, Li J, Kawabata A, Yao K, Takagi N, Okazaki M.  Cytotoxic Effects of Darinaparsin, a Novel Organic Arsenical, against Human Leukemia Cells.  Int J Mol Sci  2023  24(3)  PubMed ID: 36768603   DOI: 10.3390/ijms24032282
21195  Nakahara H, Nomura A, Tokuda S, Okamura M, Fujisawa K, Koitaya T, Yoshida Y, Furukawa S, Hitomi Y.  Superoxide Dismutase-like Activity of Zeolitic Imidazolate Framework Nanoparticles Comprising Biomimetic Imidazolato-bridged CuZn Units.  Chemistry  2023  e202300881  PubMed ID: 37096647   DOI: 10.1002/chem.202300881
21534  Ohkawa Y, Kanto N, Nakano M, Fujinawa R, Kizuka Y, Johnson EL, Harada Y, Tamura JI, Taniguchi N.  Involvement of langerin in the protective function of a keratan sulfate-based disaccharide in an emphysema mouse model.  J Biol Chem  2023  299(8):105052  PubMed ID: 37454739   DOI: 10.1016/j.jbc.2023.105052
20550  Nishimura Y, Kikuchi H, Kubo T, Arai R, Toguchi Y, Yuan B, Sunaga K, Cho H.  Synthesis of 4,4-Disubstituted 3,4-Dihydropyrimidin-2(1H)-ones and -thiones, the Corresponding Products of Biginelli Reaction Using Ketone, and Their Antiproliferative Effect on HL-60 Cells.  Chem Pharm Bull (Tokyo)  2022  70(2):111-119  PubMed ID: 35110431   DOI: 10.1248/cpb.c21-00794
20805  Saito S, Suzuki S, Arai MA.  Noaoxazole, a new heat shock metabolite produced by thermotolerant Streptomyces sp. HR41.  J Antibiot (Tokyo)  2022    PubMed ID: 35918479   DOI: 10.1038/s41429-022-00551-5
21038  Hamdy AK, Sakamoto T, Toma T, Sakamoto M, Abourehab MAS, Otsuka M, Fujita M, Tateishi H, Radwan MO.  New Insights into the Structural Requirements of Isatin-Derived Pro-Apoptotic Agents against Acute Myeloid Leukemia.  Pharmaceuticals (Basel)  2022  15(12)  PubMed ID: 36559030   DOI: 10.3390/ph15121579
20027  Ishihara K, Abe M, Fukazawa K, Konno T.  Control of Cell-Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups.  Macromol Biosci  2021  e2000341  PubMed ID: 33502108   DOI: 10.1002/mabi.202000341
20223  Yanagi K, Komatsu T, Ogihara S, Okabe T, Kojima H, Nagano T, Ueno T, Hanaoka K, Urano Y.  Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells.  Cell Rep  2021  36(1):109311  PubMed ID: 34233188   DOI: 10.1016/j.celrep.2021.109311
14828  Kohrogi K, Hino S, Sakamoto A, Anan K, Takase R, Araki H, Hino Y, Araki K, Sato T, Nakamura K, Nakao M.  LSD1 defines erythroleukemia metabolism by controlling the lineage-specific transcription factors GATA1 and C/EBPα  Blood Adv  2021  5(9):2305-2318  PubMed ID: 33929501   DOI: 10.1182/bloodadvances.2020003521
11903  Takishita Y, Yasuda H, Shimizu M, Matsuo A, Morita A, Tsutsumi T, Tsuchiya M, Sato EF.  Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells.  J Clin Biochem Nutr  2020    PubMed ID: 32001952   DOI: 10.3164/jcbn.19-77
11984  Wu Y, Zhou Y, Huang CJ, Kobayashi H, Yan S, Ozeki Y, Wu Y, Sun CW, Yasumoto A, Yatomi Y, Lei C, Goda K.  Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging.  Opt Express  2020    PubMed ID: 32118978   DOI: 10.1364/OE.380679
12132  Nemidkanam V, Kato Y, Kubota T, Chaichanawongsaroj N.  Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis.  BMC Complement Med Ther  2020    PubMed ID: 32321502   DOI: 10.1186/s12906-020-02927-2
12165  Kiyomi Hikita, Satomi Saigusa, Yuto Takeuchi, Haruka Matsuyama, Rina Nagai, Kuniki Kato, Tomiyasu Murata, Hitoshi Tanaka, Yogesh S.Wagh, Naoki Asao, Norio Kaneda  Induction of enantio-selective apoptosis in human leukemia HL-60 cells by (S)-erypoegin K, an isoflavone isolated from Erythrina poeppigiana  Bioorganic & Medicinal Chemistry  2020    PubMed ID: 32299660   DOI: 10.1016/j.bmc.2020.115490
12170  Haochen Yan, Yunzhao Wu, Yuqi Zhou, Muzhen Xu, Petra Paiè,, Cheng Lei, Sheng Yan, and Keisuke Goda  Virtual optofluidic time-stretch quantitative phase imaging  APL Photonics  2020      DOI: 10.1063/1.5134125
13003  Wang D, Sennari Y, Shen M, Morita K, Kanazawa T, Yoshida Y.  ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils.  Int Immunopharmacol  2020    PubMed ID: 32361568   DOI: 10.1016/j.intimp.2020.106510
13068  Hijioka M, Futokoro R, Ohto-Nakanishi T, Nakanishi H, Katsuki H, Kitamura Y.  Microglia-released leukotriene B4 promotes neutrophil infiltration and microglial activation following intracerebral hemorrhage.  Int Immunopharmacol  2020    PubMed ID: 32544870   DOI: 10.1016/j.intimp.2020.106678
13076  Yasuda H, Takishita Y, Morita A, Tsutsumi T, Tsuchiya M, Sato EF.  DNA demethylation increases NETosis.  Arch Biochem Biophys  2020    PubMed ID: 32561201   DOI: 10.1016/j.abb.2020.108465
13198  Hiromi I, Nakata S, Uenishi J.  Up-regulation of Death Receptor 5/TRAIL-R2 Mediates Apoptosis Induced by N,N'-[(3,4-dimethoxyphenyl)methylene] Biscinnamide in Cancer Cells.  Anticancer Res  2020    PubMed ID: 32878791   DOI: 10.21873/anticanres.14506
13431  Satoshi Yamaguchi, Risa Takagi, Tsuyoshi Hosogane, Yuki Ohashi, Yoko Sakai, Shouichi Sakakihara, Ryota Iino, Kazuhito V. Tabata, Hiroyuki Noji, and Akimitsu Okamoto  Single Cell Array Enclosed with a Photodegradable Hydrogel in Microwells for Image-Based Cell Classification and Selective Photorelease of Cells  ACS Appl Bio Mater  2020    PubMed ID: 35021817   DOI: 10.1021/acsabm.0c00583
11357  Yasuda H, Sonoda A, Yamamoto M, Kawashima Y, Takishita Y, Morita A, Tsutsumi T, Tsuchiya M, Sato EF.  17-β-estradiol enhances neutrophil extracellular trap formation by interaction with estrogen membrane receptor.  Arch. Biochem. Biophys.  2019    PubMed ID: 30590021   DOI: 10.1016/j.abb.2018.12.028
11415  Shinada H, Watanabe T, Okudaira K, Iwase Y, Nishi K, Yumita N.  Apoptosis Induced by Ultraviolet A Exposure in the Presence of Enoxacin in HL-60 Cells.  Anticancer Res.  2019    PubMed ID: 30711946   DOI: 10.21873/anticanres.13164
11442  Tabei Y, Fukui H, Nishioka A, Hagiwara Y, Sato K, Yoneda T, Koyama T, Horie M.  Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells.  Sci Rep  2019    PubMed ID: 30778158   DOI: 10.1038/s41598-019-38598-4
11619  Mizutani H, Hayashi Y, Hashimoto M, Imai M, Ichimaru Y, Kitamura Y, Ikemura K, Miyazawa D, Ohta K, Ikeda Y, Maeda T, Yoshikawa M, Hiraku Y, Kawanishi S.  Oxidative DNA Damage and Apoptosis Induced by Aclarubicin, an Anthracycline: Role of Hydrogen Peroxide and Copper.  Anticancer Res.  2019    PubMed ID: 31262868   DOI: 10.21873/anticanres.13490
11678  Yoshinori Saito, Mayu Ichihara, Koji Takiguchi, Ayumi Ohsaki, Yasuko Okamoto, Chiaki Kuroda, Xun Gong, Motoo Tori  Bisabolane, Oplopane, and Lignan Constituents of Cremanthodium campanulatum Collected in China  Natural Product Communications  2019      DOI: 10.1177/1934578X19863582
11807  Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S.  Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells.  Anticancer Res.  2019    PubMed ID: 31519583   DOI: 10.21873/anticanres.13666
11939  Okuda T, Shimizu K, Hasaba S, Date M.  Induction of specific adaptive immune responses by immunization with newly designed artificial glycosphingolipids.  Sci Rep  2019    PubMed ID: 31827147   DOI: 10.1038/s41598-019-55088-9
10616  Hikita K, Hattori N, Takeda A, Yamakage Y, Shibata R, Yamada S, Kato K, Murata T, Tanaka H, Kaneda N.  Potent apoptosis-inducing activity of erypoegin K, an isoflavone isolated from Erythrina poeppigiana, against human leukemia HL-60 cells.  J Nat Med  2018  72:260-266  PubMed ID: 29151157   DOI: 10.1007/s11418-017-1147-9
4228  Shiromizu S, Kusunose N, Matsunaga N, Koyanagi S, Ohdo S.  Optimizing the dosing schedule of l-asparaginase improves its anti-tumor activity in breast tumor-bearing mice.  J. Pharmacol. Sci.  2018    PubMed ID: 29605274   DOI: 10.1016/j.jphs.2018.01.008
10286  Oyama M, Tokiwano T, Kawaii S, Yoshida Y, Mizuno K, Oh K, Yoshizawa Y.  Protodioscin, Isolated from the Rhizome of Dioscorea tokoro Collected in Northern Japan is the Major Antiproliferative Compound to HL-60 Leukemic Cells.  Curr Bioact Compd  2017  13:170-174  PubMed ID: 28579930   DOI: 10.2174/1573407213666170113123428
10508  Kitamura H, Ishino T, Shimamoto Y, Okabe J, Miyamoto T, Takahashi E, Miyoshi I.  Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells.  Mediators Inflamm.  2017  2017:6909415  PubMed ID: 29138532   DOI: 10.1155/2017/6909415
10575  Nakai S, Imaizumi T, Watanabe T, Iwase Y, Nishi K, Okudaira K, Yumita N.  Photodynamically-induced Apoptosis Due to Ultraviolet A in the Presence of Lomefloxacin in Human Promyelocytic Leukemia Cells.  Anticancer Res.  2017  37:6407-6413  PubMed ID: 29061826   DOI: 10.21873/anticanres.12094
14731  Tomoe Yamada-Kato, Isao Okunishi, Yosuke Fukamatsu, Yusuke Yoshida  Inhibitory Effects of 6-Methylsulfinylhexyl Isothiocyanate on Superoxide Anion Generation from Differentiated HL-60 Human Promyelocytic Leukemia Cells  Food Science and Technology Research  2017  23:343-348    DOI: 10.3136/fstr.23.343
9799  Uto T, Tung NH, Thongjankaew P, Lhieochaiphant S, Shoyama Y.  Kayeassamin A Isolated from the Flower of Mammea siamensis Triggers Apoptosis by Activating Caspase-3/-8 in HL-60 Human Leukemia Cells.  Pharmacognosy Res  2016  8:244-248  PubMed ID: 27695262   DOI: 10.4103/0974-8490.188884
12623  Ishihara T, Shibui M, Hoshi T, Mizushima T.  Scavenging of superoxide anions by lecithinized superoxide dismutase in HL-60 cells  Mol Biosyst  2016  12(1):274-82  PubMed ID: 26595232   DOI: 10.1039/c5mb00631g
7333  Nishiura H, Yamanegi K, Kawabe M, Kato-Kogoe N, Yamada N, Nakasho K.  The roles of ribosomal protein S19 C-terminus in a shortened neutrophil lifespan through delta lactoferrin.  Immunobiology  2015  220:1085-92  PubMed ID: 26003841   DOI: 10.1016/j.imbio.2015.05.006
7341  Hattori T, Nakashima K, Marutani T, Kiso Y, Nishi Y, Mukai H.  Successful acquisition of a neutralizing monoclonal antibody against a?novel neutrophil-activating peptide, mitocryptide-1.  Biochem. Biophys. Res. Commun.  2015  463:54-9  PubMed ID: 25986736   DOI: 10.1016/j.bbrc.2015.05.016
7477  Ikeda Y, Yoshinari T, Nagasaki Y.  A novel biointerface that suppresses cell morphological changes by scavenging excess reactive oxygen species.  J Biomed Mater Res A  2015  103:2815-22  PubMed ID: 25691268   DOI: 10.1002/jbm.a.35419
7537  K. Tanabe, A. Yamagishi, M. Yokokawa, Y. Morimoto, M. Kinoshita, H. Suzuki  Microdevice for the measurement of neutrophil activity for stress monitoring.  2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)  2015  1707-1710    DOI: 10.1109/TRANSDUCERS.2015.7181273
14951  Hikita K, Yamada S, Shibata R, Katoh M, Murata T, Kato K, Tanaka H, Kaneda N.  Inhibitory Effect of Isoflavones from Erythrina poeppigiana on the Growth of HL-60 Human Leukemia Cells through Inhibition of Glyoxalase I  Nat Prod Commun  2015  10(9):1581-4  PubMed ID: 26594764  

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