検索画面
細胞材料開発室サイト

戻る 戻る
HPS0063 : 201B7  update : 2018/10/18
CommentHuman iPS cell line. HPS0002 and HPS0076 were derived from the same patient.Order Form (C-0042, C-0034). Please see CiRA , Kyoto University if you are a researcher at a for–profit organization.
特性(日) ヒト人工多能性幹(iPS)細胞株。HPS0002、HPS0076と同一人。レトロウイルスベクターにより4因子(Oct3/4,Sox2,Klf4,c-Myc)を導入。前提供番号HPS0001 。 ガラス化法(Vitrification法)保存。営利機関は京都大学iPS細胞研究所から配布。
寄託者特性(英)
寄託者特性(日)
使用条件f
使用条件(英)1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
使用条件(日)1) 利用者は学術機関に所属している者に限る。2) 別紙1を提供同意書 (C-0028) に添付すること。
提供申込書類 依頼書C-0041.pdf   同意書C-0028.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 http://www.cira.kyoto-u.ac.jp/
樹立者 Yamanaka, Shinya
寄託日 2011
別名 HPS0001 201B7
動物種 human < Mammals
属名 Homo
種名 sapiens
人種 Caucasian
性別 Female
36 years
採取組織 skin
細胞分類 iPS
遺伝子改変の有無 recombinant
外来遺伝子 pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc, pLenti6/UbC/V5-DEST, mouse Slc7a1
細胞寿命 infinite
細胞形態 ES-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 Primate ES Cell Medium + 4ng/ml human basic FGF
抗生物質 Free
継代方法 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
培養容器のコーティング 0.1% gelatin coated dish
継代密度 1 : 3-6 split
継代または培地交換頻度 Subculture : once/4-6 days, Medium Renewal : every day
培養最適温度 37 ℃
二酸化炭素濃度 5 %
フィーダー細胞 SNL 76/7
フィーダー細胞の処理方法 X-rays 5000R (or MMC)
フィーダー細胞の播種数 1.5-2.5 x 10^(6) cells/100 mm dish
凍結培地 DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
凍結方法 Vitrification
マイコプラズマ (-)
個体識別検査 OK
染色体検査 46XX(20)
画像情報
寄託時情報
ロット情報
その他の細胞情報 寄託時情報ロット情報
SDS_DAP(MEM)_Japanese.pdf
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 65件
利用者成果(日) 1件

トップへページトップへ
Reference(英)

トップへページトップへ
Reference(日)

トップへページトップへ
利用者成果(英)
Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH.  Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.  Sci Rep  2018  8:1189  PubMed ID: 29352121
Yamamoto T, Takenaka C, Yoda Y, Oshima Y, Kagawa K, Miyajima H, Sasaki T, Kawamata S.  Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7.  Sci Rep  2018  8:241  PubMed ID: 29321579
Taguchi A, Nishinakamura R.  Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.  Cell Stem Cell  2017    PubMed ID: 29129523
Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.  CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.  Nat. Methods  2017  14:963-966  PubMed ID: 28892089
Goparaju SK, Kohda K, Ibata K, Soma A, Nakatake Y, Akiyama T, Wakabayashi S, Matsushita M, Sakota M, Kimura H, Yuzaki M, Ko SB, Ko MS.  Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors.  Sci Rep  2017  7:42367  PubMed ID: 28205555
Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T.  Five colour variants of bright luminescent protein for real-time multicolour bioimaging.  Nat Commun  2016  7:13718  PubMed ID: 27966527
Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835
Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.  TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.  Sci Rep  2016  6:21747  PubMed ID: 26888607
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  Involvement of the Wnt signaling pathway in feeder?free culture of human induced pluripotent stem cells.  Mol Med Rep  2015  12:6797-800  PubMed ID: 26398905
Arai Y, Hayakawa K, Arai D, Ito R, Iwasaki Y, Saito K, Akutsu K, Takatori S, Ishii R, Hayashi R, Izumi S, Sugino N, Kondo F, Horie M, Nakazawa H, Makino T, Hirosawa M, Shiota K, Ohgane J.  Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells.  Biomed Res Int  2015  2015:876047  PubMed ID: 26339649
Fujiwara N, Shimizu J, Takai K, Arimitsu N, Ueda Y, Wakisaka S, Suzuki T, Suzuki N.  Cellular and molecular mechanisms of the restoration of human APP transgenic mouse cognitive dysfunction after transplant of human iPS cell-derived neural cells.  Exp. Neurol.  2015  271:423-31  PubMed ID: 26196079
Imaizumi K, Iha M, Nishishita N, Kawamata S, Nishikawa S, Akuta T.  A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.  Methods Mol. Biol.  2015  1341:15-24  PubMed ID: 26069023
Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K.  Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.  Methods Mol. Biol.  2015  1357:111-31  PubMed ID: 26025620
Tateno H, Onuma Y, Ito Y, Minoshima F, Saito S, Shimizu M, Aiki Y, Asashima M, Hirabayashi J.  Elimination of tumorigenic human pluripotent stem cells by a recombinant lectin-toxin fusion protein.  Stem Cell Reports  2015  4:811-20  PubMed ID: 25866158
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  An Optimal Medium Supplementation Regimen for Initiation of Hepatocyte Differentiation in Human Induced Pluripotent Stem Cells.  J. Cell. Biochem.  2015  116:1479-89  PubMed ID: 25683148
Kato H, Ochiai-Shino H, Onodera S, Saito A, Shibahara T, Azuma T.  Promoting effect of 1,25(OH)2 vitamin D3 in osteogenic differentiation from induced pluripotent stem cells to osteocyte-like cells.  Open Biol  2015  5:140201  PubMed ID: 25652541
Binata Joddar, Chieko Nishioka, Eiki Takahashi, Yoshihiro Ito  Chemically fixed autologous feeder cell-derived niche for human induced pluripotent stem cell culture.  J Mater Chem B.  2015  3:2301-2307 
M Iinuma, T Umehara, N Arimitsu, J Shimizu, H Misawa, K Takai, N Fujiwara, A Fujii, Y Ueda, S Wakisaka, T Suzuki, C Hirotsu, M Beppu, N Suzuki  Induction of neural cells with spinal motoneuron phenotype from human iPS cells and the trans-plantation to totally transected spinal cords in mice.  Inflammation and Regeneration  2015  35:154-163 
N Kuzumaki, M Narita  δ-Opioids and Neurogenesis.  Y. Xia (ed.), Neural Functions of the Delta-Opioid Receptor  2015  Chapter 9:389-398 
Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23 
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  Transcription Factors and Medium Suitable for Initiating the Differentiation of Human-Induced Pluripotent Stem Cells to the Hepatocyte Lineage.  J. Cell. Biochem.  2016  117:2001-9  PubMed ID: 26773721
Shoji M, Nishioka M, Minato H, Harada K, Kubo M, Fukuyama Y, Kuzuhara T.  Neurotrophic activity of jiadifenolide on neuronal precursor cells derived from human induced pluripotent stem cells.  Biochem. Biophys. Res. Commun.  2016  470:798-803  PubMed ID: 26809091
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  Improved Survival and Initiation of Differentiation of Human Induced Pluripotent Stem Cells to Hepatocyte-Like Cells upon Culture in William's E Medium followed by Hepatocyte Differentiation Inducer Treatment.  PLoS ONE  2016  11:e0153435  PubMed ID: 27073925
Horiguchi I, Sakai Y.  Serum replacement with albumin-associated lipids prevents excess aggregation and enhances growth of induced pluripotent stem cells in suspension culture.  Biotechnol. Prog.  2016  32:1009-16  PubMed ID: 27193385
Bamba Y, Shofuda T, Kato M, Pooh RK, Tateishi Y, Takanashi J, Utsunomiya H, Sumida M, Kanematsu D, Suemizu H, Higuchi Y, Akamatsu W, Gallagher D, Miller FD, Yamasaki M, Kanemura Y, Okano H.  In vitro characterization of neurite extension using induced pluripotent stem cells derived from lissencephaly patients with TUBA1A missense mutations.  Mol Brain  2016  9:70  PubMed ID: 27431206
Shimba K, Iida S, Kotani K, Jimbo Y.  Cell-cycle-dependent Ca2+ transients in human induced pluripotent stem cells revealed by a simultaneous imaging of cell nuclei and intracellular Ca2+ level.  Integr Biol (Camb)  2016  8:985-990  PubMed ID: 27713967
Seno A, Kasai T, Ikeda M, Vaidyanath A, Masuda J, Mizutani A, Murakami H, Ishikawa T, Seno M.  Characterization of Gene Expression Patterns among Artificially Developed Cancer Stem Cells Using Spherical Self-Organizing Map.  Cancer Inform  2016  15:163-78  PubMed ID: 27559294
Suga M, Tachikawa S, Tateyama D, Ohnuma K, Furue MK.  Imaging-cytometry revealed spatial heterogeneities of marker expression in undifferentiated human pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2017  53:83-91  PubMed ID: 27573412
Tomotsune D, Hirashima K, Fujii M, Yue F, Matsumoto K, Takizawa-Shirasawa S, Yokoyama T, Sasaki K.  Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment.  PLoS ONE  2016  11:e0162693  PubMed ID: 27632182
Ozeki N, Hase N, Higuchi N, Hiyama T, Yamaguchi H, Kawai R, Matsumoto T, Nakata K, Mogi M.  Gelatin scaffold combined with bone morphogenetic protein-4 induces odontoblast-like cell differentiation involving integrin profile changes, autophagy-related gene 10, and Wnt5 sequentially in human induced pluripotent stem cells.  Differentiation    93:1-14  PubMed ID: 27639333
Imamura T, Fujita K, Tagawa K, Ikura T, Chen X, Homma H, Tamura T, Mao Y, Taniguchi JB, Motoki K, Nakabayashi M, Ito N, Yamada K, Tomii K, Okano H, Kaye J, Finkbeiner S, Okazawa H.  Identification of hepta-histidine as a candidate drug for Huntington's disease by in silico-in vitro- in vivo-integrated screens of chemical libraries.  Sci Rep  2016  6:33861  PubMed ID: 27653664
Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai-Asano R, Nakata K, Mogi M.  MicroRNA-211 and autophagy-related gene 14 signaling regulate osteoblast-like cell differentiation of human induced pluripotent stem cells.  Exp. Cell Res.  2017  352:63-74  PubMed ID: 28159471
Nagasaka R, Gotou Y, Yoshida K, Kanie K, Shimizu K, Honda H, Kato R.  Image-based cell quality evaluation to detect irregularities under same culture process of human induced pluripotent stem cells.  J. Biosci. Bioeng.  2017  123:642-650  PubMed ID: 28189491
Haraguchi Y, Hasegawa A, Matsuura K, Kobayashi M, Iwana SI, Kabetani Y, Shimizu T.  Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography.  Biomed Res Int  2017  2017:5341702  PubMed ID: 28326324
Takahashi M, Saito A, Jimbo Y, Nakasono S.  Evaluation of the effects of power-frequency magnetic fields on the electrical activity of cardiomyocytes differentiated from human induced pluripotent stem cells.  J Toxicol Sci  2017  42:223-231  PubMed ID: 28321048
Kondo Y, Hattori K, Tashiro S, Nakatani E, Yoshimitsu R, Satoh T, Sugiura S, Kanamori T, Ohnuma K.  Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates.  J. Biosci. Bioeng.  2017  124:234-241  PubMed ID: 28434976
Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  Differentiation of human induced pluripotent stem cells in William's E initiation medium supplemented with 3‑bromopyruvate and 2‑deoxy‑d‑glucose.  Mol Med Rep  2017  15:3719-3723  PubMed ID: 28440498
Ishikawa T.  Next-generation sequencing traces human induced pluripotent stem cell lines clonally generated from heterogeneous cancer tissue.  World J Stem Cells  2017  9:77-88  PubMed ID: 28596815
Bamba Y, Kanemura Y, Okano H, Yamasaki M.  Visualization of migration of human cortical neurons generated from induced pluripotent stem cells.  J. Neurosci. Methods  2017  289:57-63  PubMed ID: 28694214
Tateno H, Saito S.  Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells.  Molecules  2017  22:  PubMed ID: 28698527
Taniguchi J, Pandian GN, Hidaka T, Hashiya K, Bando T, Kim KK, Sugiyama H.  A synthetic DNA-binding inhibitor of SOX2 guides human induced pluripotent stem cells to differentiate into mesoderm.  Nucleic Acids Res.  2017  45:9219-9228  PubMed ID: 28934500
Tachikawa S, Nishimura T, Nakauchi H, Ohnuma K.  Thalidomide induces apoptosis in undifferentiated human induced pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2017  53:841-851  PubMed ID: 28849348
Hosoyama T, Ichida S, Kanno M, Ishihara R, Hatashima T, Ueno K, Hamano K.  Microgravity influences maintenance of the human muscle stem/progenitor cell pool.  Biochem. Biophys. Res. Commun.  2017  493:998-1003  PubMed ID: 28942144
Matsumura W, Fujita Y, Nakayama C, Shinkuma S, Suzuki S, Nomura T, Abe R, Shimizu H.  Establishment of integration-free induced pluripotent stem cells from human recessive dystrophic epidermolysis bullosa keratinocytes.  J. Dermatol. Sci.  2018  89:263-271  PubMed ID: 29229433
Bamba Y, Nonaka M, Sasaki N, Shofuda T, Kanematsu D, Suemizu H, Higuchi Y, Pooh RK, Kanemura Y, Okano H, Yamasaki M.  Generation of Induced Pluripotent Stem Cells and Neural Stem/Progenitor Cells from Newborns with Spina Bifida Aperta.  Asian Spine J  2017  11:870-879  PubMed ID: 29279741
Kavitha MS, Kurita T, Park SY, Chien SI, Bae JS, Ahn BC.  Deep vector-based convolutional neural network approach for automatic recognition of colonies of induced pluripotent stem cells.  PLoS ONE  2017  12:e0189974  PubMed ID: 29281701
Suda Y, Kuzumaki N, Sone T, Narita M, Tanaka K, Hamada Y, Iwasawa C, Shibasaki M, Maekawa A, Matsuo M, Akamatsu W, Hattori N, Okano H, Narita M.  Down-regulation of ghrelin receptors on dopaminergic neurons in the substantia nigra contributes to Parkinson's disease-like motor dysfunction.  Mol Brain  2018  11:6  PubMed ID: 29458391
Saito S, Hiemori K, Kiyoi K, Tateno H.  Glycome analysis of extracellular vesicles derived from human induced pluripotent stem cells using lectin microarray.  Sci Rep  2018  8:3997  PubMed ID: 29507392
Sugimoto K, Matsuura T, Nakazono A, Igawa K, Yamada S, Hayashi Y.  Effects of hypoxia inducible factors on pluripotency in human iPS cells.  Microsc. Res. Tech.  2018    PubMed ID: 29633433
Lee JY, Han SH, Park MH, Baek B, Song IS, Choi MK, Takuwa Y, Ryu H, Kim SH, He X, Schuchman EH, Bae JS, Jin HK.  Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease.  Nat Commun  2018  9:1479  PubMed ID: 29662056
Tashiro S, Le MNT, Kusama Y, Nakatani E, Suga M, Furue MK, Satoh T, Sugiura S, Kanamori T, Ohnuma K.  High cell density suppresses BMP4-induced differentiation of human pluripotent stem cells to produce macroscopic spatial patterning in a unidirectional perfusion culture chamber.  J. Biosci. Bioeng.  2018    PubMed ID: 29681444
  Transplantation of human-induced pluripotent stem cells carried by self-assembling peptide nanofiber hydrogel improves bone regeneration in rat calvarial bone defects        PubMed ID:
  Human iPS cell derived neural cell sheets exhibit mature neural and extendable scaffold functions and promote recovery in injured mouse spinal cords        PubMed ID:
  Plasma-activated medium selectively eliminates undifferentiated human induced pluripotent stem cells        PubMed ID:
  Human iPS cell derived neural cell sheets exhibit mature neural and extendable scaffold functions and promote recovery in injured mouse spinal cords        PubMed ID:
  ROCK INHIBITOR Y-27632 USED DURING CELL AGGREGATE FORMATION ADVERSELY AFFECTS THE EARLY CARDIAC DIFFERENTIATION OF HUMAN iPS CELLS        PubMed ID:
  Method and kit for detecting stem cell        PubMed ID:
  METHOD FOR PRODUCING NAÏVE PLURIPOTENT STEM CELLS        PubMed ID:
  METHOD FOR SORTING TISSUE CELLS        PubMed ID:
Arioka Y, Shishido E, Kubo H, Kushima I, Yoshimi A, Kimura H, Ishizuka K, Aleksic B, Maeda T, Ishikawa M, Kuzumaki N, Okano H, Mori D, Ozaki N.  Single-cell trajectory analysis of human homogenous neurons carrying a rare RELN variant.  Transl Psychiatry  2018    PubMed ID: 30022058
Nagashima T, Shimizu K, Matsumoto R, Honda H.  Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine.  Sci Rep  2018    PubMed ID: 30127448
Yamamoto Y, Miyazaki S, Maruyama K, Kobayashi R, Le MNT, Kano A, Kondow A, Fujii S, Ohnuma K.  Random migration of induced pluripotent stem cell-derived human gastrulation-stage mesendoderm.  PLoS ONE  2018    PubMed ID: 30199537
Tsuburaya N, Homma K, Higuchi T, Balia A, Yamakoshi H, Shibata N, Nakamura S, Nakagawa H, Ikeda SI, Umezawa N, Kato N, Yokoshima S, Shibuya M, Shimonishi M, Kojima H, Okabe T, Nagano T, Naguro I, Imamura K, Inoue H, Fujisawa T, Ichijo H.  A small-molecule inhibitor of SOD1-Derlin-1 interaction ameliorates pathology in an ALS mouse model.  Nat Commun  2018    PubMed ID: 29991716
Yoda K, Ohnuki Y, Kurosawa H.  Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions.  J. Biosci. Bioeng.  2018    PubMed ID: 30292671
Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143

トップへページトップへ
利用者成果(日)
  ヒト iPS 細胞の分化誘導プロセスにおける細胞の構造構築段階の重要性        PubMed ID:



戻る 戻る 理研トップページへ