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Cell No. : Cell Name
HPS4290 : 201B7-Ff  update : 2021/11/17
CommentHuman iPS cell line derived from a healthy individual. HPS0002, HPS0063, HPS0076, HPS0296, HPS0326, HPS3668, HPS4290, HPS4291, HPS4292 were derived from the same person.
Comment from the depositorHuman iPS cell line. This line was established by introduction of retroviral vectors encoding 4 factors (Oct3/4, Sox2, Klf4, c-Myc). This line was on-feeder culture but moved to feeder-less.
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach TERMS and CONDITIONS (non-for-profit) or TERMS and CONDITIONS (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Expected time
Year of deposit 2019
Animal _human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 36
Tissue skin
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc
Vector MMLV retroviral vector (pMXs)
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm^(2) Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10^(4) cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium STEM-CELLBANKER
Freezing method Slow freezing
Mycoplasma (-)
Chromosome mode 46,XX[20]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Reference information Reference 0
User's Publication 0


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