Cell No. : Cell Name
HPS4290 : 201B7-Ff
update : 2024/04/09
|
| Comment | Human iPS cell line derived from a healthy individual. HPS0002, HPS0063, HPS0076, HPS0296, HPS0326, HPS3668, HPS4290, HPS4291, HPS4292 were derived from the same person. |
| Comment from the depositor | Human iPS cell line. This line was established by introduction of retroviral vectors encoding 4 factors (Oct3/4, Sox2, Klf4, ｃ-Myc). This line was on-feeder culture but moved to feeder-less. |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Expected time |
|
| Year of deposit |
2019
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Caucasian
|
| Gender |
Female
|
| Age at sampling |
36
|
| Tissue |
skin
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, c-Myc
|
| Vector |
MMLV retroviral vector (pMXs)
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
|
Culture information
|
Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
STEM-CELLBANKER
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Chromosome mode |
|
46,XX[20]
|
| Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
3
|
| User's Publication |
|
19706
Bamba K, Ozawa M, Daitoku H, Kohara A.
Diverting the food-freezing technology improves the cryopreservation efficiency of induced pluripotent stem cells and derived neurospheres
Regen Ther
2024
27:83-91
PubMed ID: 38525239
DOI: 10.1016/j.reth.2024.03.007
|
|
21571
Nakamura T, Ueda J, Mizuno S, Honda K, Kazuno AA, Yamamoto H, Hara T, Takata A.
Topologically associating domains define the impact of de novo promoter variants on autism spectrum disorder risk.
Cell Genom
2024
100488
PubMed ID: 38280381
DOI: 10.1016/j.xgen.2024.100488
|
|
19863
Nakano-Kobayashi A, Canela A, Yoshihara T, Hagiwara M.
Astrocyte-targeting therapy rescues cognitive impairment caused by neuroinflammation via the Nrf2 pathway
Proc Natl Acad Sci U S A
2023
120(33):e2303809120
PubMed ID: 37549281
DOI: 10.1073/pnas.2303809120
|
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