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Cell No. : Cell Name
RCB0435 : U-937 DE-4  update : 2021/05/25
CommentCloned U-937, differentiate with TNF
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Iwamoto, Sanju
Originator Sundstrom, C. & Nilsson, K.
Year of deposit 1990
Another name DE-4
Original cell U-937
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 37 years
Tissue pleural effusion
Disease name histiocyte lymphoma
Classification cancer
History J. Minowada (RPMI)
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8765
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 40-62(50) : /49(3),50(5),51(10),52(6),53(6),54(5)/
STR(human) OK
Reference information Reference 6
User's Publication 51


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Reference
3507  Biochem Biophys Res Commun  Lymphotoxin lacks effects on 75-kDa receptors in cytotoxicity on U-937 cells.  Iwamoto S, Shibuya I, Takeda K, Takeda M.  1994  199(1):70-7  PubMed ID: 8123048
3505  Iwamoto S, Takeda K, Kamijo R, Konno K.  Induction of resistance to TNF cytotoxicity and mitochondrial superoxide dismutase on U-937 cells by 1,25-dihydroxyvitamin D3.  Biochem Biophys Res Commun  1990  170(1):73-9  PubMed ID: 2372299
3506  Iwanami M, Takeda K, Iwamoto S, Konno K.  Combination effects of interferon-gamma and cholera toxin on induction of differentiation of an insensitive U-937 clone.  Jpn J Cancer Res  1990  81(5):520-6  PubMed ID: 2166023
1143  Fischer, D G, Pike, M C, Koren, H S, Snyderman R  Chemotactically responsive and nonresposive forms of a continuous human monocyte cell line.  J Immunol  1980  125:463-5  PubMed ID: 7381208
1142  Koren, H S, Anderson, S J, Larrick, J W  In vitro activation of a human macrophage-like cell line.  Nature  1979  279:328-31  PubMed ID: 450085
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890

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User's Publication
14198  Zha S, Ueno M, Liang Y, Okada S, Oda T, Ishibashi F.  Induction of Apoptotic Cell Death in Human Leukemia U937 Cells by C18 Hydroxy Unsaturated Fatty Acid Isolated from Red Alga Tricleocarpa jejuensis  Mar Drugs  2021  19(3):138  PubMed ID: 33801204
14296  Yambe N, Tamai R, Mashima I, Kiyoura Y.  Etidronate down-regulates Toll-like receptor 2 ligand-induced chemokine production by inhibiting MyD88 expression and NF-κB activation  Immunopharmacol Immunotoxicol  2021  43(1):51-57.  PubMed ID: 33251898
14477  Zhao Y, Zhu XY, Song T, Zhang L, Eirin A, Conley S, Tang H, Saadiq I, Jordan K, Lerman A, Lerman LO.  Mesenchymal stem cells protect renal tubular cells via TSG-6 regulating macrophage function and phenotype switching  Am J Physiol Renal Physiol  2021  320(3):F454-F463  PubMed ID: 33554782
14475  Hidehiko Kikuchi, Kaori Harata, Chikage Kawai, Harishkumar Madhyastha, Akira Yamauchi, Futoshi Kuribayashi  Retinoic acid dramatically enhances cytotoxicity of equol against human monoblastic U937 cells, but not against human peripheral neutrophils  Fundamental Toxicological Sciences  2020  7:201-206 
6891  Sumiko Akiyoshi, Hidehiko Kikuchi, Futoshi Kuribayashi, Harishkumar Madhyastha, Hisanori Minami  Sulforaphane displays the growth inhibition, cytotoxicity and enhancement of retinoic acid-induced superoxide-generating activity in human monoblastic U937 cells  Fundamental Toxicological Sciences  2019  6:319-325 
9138  Aki Kawano, Wataru Ariyoshi, Yoshie Yoshioka, Hisako Hikiji, Tatsuji Nishihara, Toshinori Okinaga  Docosahexaenoic Acid Enhances M2 Macrophage Polarization via the p38 Signaling Pathway and Autophagy  J Cell Biochem  2019  120 (8), 12604-12617  PubMed ID: 30825233
9810  Junko Shibato, Fumiko Takenoya, Takahiro Hirabayashi, Ai Kimura, Yusuke Iwasaki, Yoko Toyoda, Motohide Hori, Shigeru Tamogami, Randeep Rakwal, Seiji Shioda  Towards Identification of Bioactive Compounds in Cold Vacuum Extracted Double Cherry Blossom (Gosen-Sakura) Leaves  Plant Signal Behav  2019  14(10):e1644594  PubMed ID: 31342859
10626  Kikuchi H, Mimuro H, Kuribayashi F.  Resveratrol strongly enhances the retinoic acid-induced superoxide generating activity via up-regulation of gp91-phox gene expression in U937 cells.  Biochem. Biophys. Res. Commun.  2018  495:1195-1200  PubMed ID: 29183727
4445  Hidehiko Kikuchi, Futoshi Kuribayashi, Harishkumar Madhyastha  Positions and numbers of hydroxyl groups in hydroxychalcone derivatives are involved in cytotoxicity against human monoblastic U937 cells  Fundamental Toxicological Sciences  2018  5, 149-152 
14473  Nakagawa M, Uno S, Iriyama N, Matsunawa M, Makishima M, Takeuchi J, Tsuboi I, Hatta Y, Takei M.  Combined treatment with benzo[a]pyrene and 1α,25-dihydroxyvitamin D 3 induces expression of plasminogen activator inhibitor 1 in monocyte/macrophage-derived cells  Toxicol Appl Pharmacol  2018  345:48-56  PubMed ID: 29524502
10267  Tsunaka M, Shinki H, Koyama T.  Cell-based evaluation of changes in coagulation activity induced by antineoplastic drugs for the treatment of acute myeloid leukemia.  PLoS ONE  2017  12:e0175765  PubMed ID: 28406995
14457  Okamura Y, Otani K, Sekiguchi A, Kogane T, Kakuda C, Sakamoto Y, Kodama T, Okada M, Yamawaki H.  Vasculo-protective effect of BMS-309403 is independent of its specific inhibition of fatty acid-binding protein 4  Pflugers Arch  2017  469(9):1177-1188  PubMed ID: 28405802
14458  Yamamichi K, Fukuda T, Sanui T, Toyoda K, Tanaka U, Nakao Y, Yotsumoto K, Yamato H, Taketomi T, Uchiumi T, Nishimura F.  Amelogenin induces M2 macrophage polarisation via PGE2/cAMP signalling pathway  Arch Oral Biol  2017  83:241-251  PubMed ID: 28822800
14470  Terukazu Sanui, Takao Fukuda, Kensuke Yamamichi, Kyosuke Toyoda, Urara Tanaka, Karen Yotsumoto, Takaharu Taketomi, Fusanori Nishimura  Microarray Analysis of the Effects of Amelogenin on U937 Monocytic Cells  American Journal of Molecular Biology  2017  07:107-122 
14471  Otsuka M, Okinaga T, Ariyoshi W, Kitamura C, Nishihara T.  Ameloblastin Upregulates Inflammatory Response Through Induction of IL-1β in Human Macrophages  J Cell Biochem  2017  118(10):3308-3317  PubMed ID: 28295583
14035  Sumiya YU, Inoue T, Ishikawa M, Inui T, Kuchiike D, Kubo K, Uto Y, Nishikata T.  Macrophages Exhibit a Large Repertoire of Activation States via Multiple Mechanisms of Macrophage-activating Factors  Anticancer Res  2016  36(7):3619-23.  PubMed ID: 27354632
12240  Hirofumi Sawai, Hideo Ogiso, Toshiro Okazaki  Differential changes in sphingolipids between TNF-induced necroptosis and apoptosis in U937 cells and necroptosis-resistant sublines  Leuk Res  2015  39(9):964-70  PubMed ID: 26189109
14463  Taniguchi K, Hikiji H, Okinaga T, Hashidate-Yoshida T, Shindou H, Ariyoshi W, Shimizu T, Tominaga K, Nishihara T.  Essential Role of Lysophosphatidylcholine Acyltransferase 3 in the Induction of Macrophage Polarization in PMA-Treated U937 Cells  J Cell Biochem  2015  116(12):2840-8  PubMed ID: 25994902
4993  Tsubaki M, Itoh T, Satou T, Imano M, Komai M, Ogawa N, Mukai J, Nishida S.  Nitrogen-containing bisphosphonates induce apoptosis of hematopoietic tumor cells via inhibition of Ras signaling pathways and Bim-mediated activation of the intrinsic apoptotic pathway.  Biochem Pharmacol  2013  85(2):163-72  PubMed ID: 23085435
13725  Abe K, Ichinomiya R, Kanai T, Yamamoto K.  Effect of a Japanese energy healing method known as Johrei on viability and proliferation of cultured cancer cells in vitro  J Altern Complement Med  2012  18(3):221-8  PubMed ID: 22385045
7684  Kim SH, Yamamoto T, Fourmy D, Fujii T.  Electroactive microwell arrays for highly efficient single-cell trapping and analysis.  Small  2011  7:3239-47  PubMed ID: 21932278
7752  Sawai H, Domae N.  Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells.  Biochem. Biophys. Res. Commun.  2011  411:569-73  PubMed ID: 21763280
7765  Herlambang S, Kumagai M, Nomoto T, Horie S, Fukushima S, Oba M, Miyazaki K, Morimoto Y, Nishiyama N, Kataoka K.  Disulfide crosslinked polyion complex micelles encapsulating dendrimer phthalocyanine directed to improved efficiency of photodynamic therapy.  J Control Release  2011  155:449-57  PubMed ID: 21704092
7778  Phalitakul S, Okada M, Hara Y, Yamawaki H.  Vaspin prevents TNF-α-induced intracellular adhesion molecule-1 via inhibiting reactive oxygen species-dependent NF-κB and PKCθ activation in cultured rat vascular smooth muscle cells.  Pharmacol. Res.  2011  64:493-500  PubMed ID: 21683791
7822  Aida J, Higuchi S, Hasegawa Y, Nagano-Ito M, Hirabayashi Y, Banba A, Shimizu T, Kikuchi A, Saga M, Ichikawa S.  Up-regulation of ceramide glucosyltransferase during the differentiation of U937 cells.  J. Biochem.  2011  150:303-10  PubMed ID: 21558327
7845  Yamawaki H, Kuramoto J, Kameshima S, Usui T, Okada M, Hara Y.  Omentin, a novel adipocytokine inhibits TNF-induced vascular inflammation in human endothelial cells.  Biochem. Biophys. Res. Commun.  2011  408:339-43  PubMed ID: 21514279
8227  Tsubosaka Y, Murata T, Yamada K, Uemura D, Hori M, Ozaki H.  Halichlorine reduces monocyte adhesion to endothelium through the suppression of nuclear factor-kappaB activation.  J. Pharmacol. Sci.  2010  113:208-13  PubMed ID: 20562517
4869  Kitagawa J, Hara T, Tsurumi H, Kanemura N, Kasahara S, Shimizu M, Moriwaki H.  Cell cycle-dependent priming action of granulocyte colony-stimulating factor (G-CSF) enhances in vitro apoptosis induction by cytarabine and etoposide in leukemia cell lines.  J Clin Exp Hematop  2010  50(2):99-105  PubMed ID: 21123967
6125  Shimahara A, Yamakawa N, Nishikata I, Morishita K.  Acetylation of lysine 564 adjacent to the C-terminal binding protein-binding motif in EVI1 is crucial for transcriptional activation of GATA2.  J Biol Chem  2010  285(22):16967-77  PubMed ID: 20363750
8505  Khuda II, Koide N, Noman AS, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.  Astrocyte elevated gene-1 (AEG-1) is induced by lipopolysaccharide as toll-like receptor 4 (TLR4) ligand and regulates TLR4 signalling.  Immunology  2009  128:e700-6  PubMed ID: 19740331
8579  Amano Y, Cho Y, Matsunawa M, Komiyama K, Makishima M.  Increased nuclear expression and transactivation of vitamin D receptor by the cardiotonic steroid bufalin in human myeloid leukemia cells.  J. Steroid Biochem. Mol. Biol.  2009  114:144-51  PubMed ID: 19429444
8602  Nakayasu S, Soegima R, Yamaguchi K, Oda T.  Biological activities of fucose-containing polysaccharide ascophyllan isolated from the brown alga Ascophyllum nodosum.  Biosci. Biotechnol. Biochem.  2009  73:961-4  PubMed ID: 19352011
6436  Hisao Haniu, Yoshikazu Matsuda, Kenji Takeuchi  Potential of a Novel Safety Evaluation of Nanomaterials Using a Proteomic Approach  Journal of Health Science  2009  55:428-434 
9068  Yasuhiro Yamasaki, Daisuke Katsuo, Seiichiro Nakayasu, Cristina Salati, JingJing Duan, Yanan Zou, Yukihiko Matsuyama, Kenichi Yamaguchi, Tatsuya Oda  Purification and Characterization of a Novel High Molecular Weight Exotoxin Produced by Red Tide Phytoplankton, Alexandrium Tamarense  J Biochem Mol Toxicol  2008  22 (6), 405-15  PubMed ID: 19111002
2808  Pang, Zhongcun, Otaka, Kodo, Maoka, Takashi, Hidaka, Kumi, Ishijima, Sumio, Oda, Masayuki, Ohnishi, Masatake  Structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of U937 cells.  Biosci Biotechnol Biochem  2005  69:553-8  PubMed ID: 15784984
3270  Inamura J, Ikuta K, Jimbo J, Shindo M, Sato K, Torimoto Y, Kohgo Y.  Upregulation of hepcidin by interleukin-1beta in human hepatoma cell lines.  Hepatol. Res.  2005  33:198-205  PubMed ID: 16198622
9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112
12837  Fujita H, Utsumi T, Muranaka S, Ogino T, Yano H, Akiyama J, Yasuda T, Utsumi K.  Involvement of Ras/extracellular signal-regulated kinase, but not Akt pathway in risedronate-induced apoptosis of U937 cells and its suppression by cytochalasin B  Biochem Pharmacol  2005  69(12):1773-84.  PubMed ID: 15869745
2848  Feng, Xingmin, Chuhjo, Tatsuya, Sugimori, Chiharu, Kotani, Takeharu, Lu, Xuzhang, Takami, Akiyoshi, Takamatsu, Hiroyuki, Yamazaki, Hirohito, Nakao, Shinji  Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells.  Blood  2004  104:2425-31  PubMed ID: 15217832
5409  Fukushima K, Ishiyama C, Yamashita K.  Recognition by TNF-alpha of the GPI-anchor glycan induces apoptosis of U937 cells.  Arch Biochem Biophys  2004  426(2):298-305  PubMed ID: 15158680
13883  Kobayashi H, Takemura Y, Hayashi T, Ujiiye T, Kawase M, Niino Y, Miyachi H, Ohshima T, Hotta T.  Expression level of MDR1 message in peripheral blood leukocytes from healthy adults: a competitive nucleic acid sequence-based amplification assay for its determination  Clin Chem Lab Med  2004  42(10):1098-101  PubMed ID: 15552266
2662  Akiyama, Hidehiko, Ino, Teruo, Tokunaga, Etsuko, Katsuda, Itsurou, Ezaki, Kohji  A synergistic increase of apoptosis utilizing Fas antigen expression induced by low doses of anticancer drug.  Rinsho Byori  2003  51:733-9  PubMed ID: 13677932
3979  Matsumoto K, Akao Y, Kobayashi E, Ohguchi K, Ito T, Tanaka T, Iinuma M, Nozawa Y.  Induction of apoptosis by xanthones from mangosteen in human leukemia cell lines.  J Nat Prod  2003  66(8):1124-7  PubMed ID: 12932141
6708  Hikasa M, Yamamoto E, Kawasaki H, Komai K, Shiozawa K, Hashiramoto A, Miura Y, Shiozawa S.  p21waf1/cip1 is down-regulated in conjunction with up-regulation of c-Fos in the lymphocytes of rheumatoid arthritis patients.  Biochem Biophys Res Commun  2003  304(1):143-7  PubMed ID: 12705898
2549  Nagai, Fusako, Hiyoshi, Yasuko, Sugimachi, Kumiko, Tamura, Hiro-Omi  Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines.  Biol Pharm Bull  2002  25:383-5  PubMed ID: 11913539
9032  N Kiyokawa, T Mori, T Taguchi, M Saito, K Mimori, T Suzuki, T Sekino, N Sato, H Nakajima, Y U Katagiri, T Takeda, J Fujimoto  Activation of the Caspase Cascade During Stx1-induced Apoptosis in Burkitt's Lymphoma Cells  J Cell Biochem  2001  81 (1), 128-42  PubMed ID: 11180403
9021  N Hasegawa, Y Kimura, T Oda, N Komatsu, T Muramatsu  Isolated Ricin B-chain-mediated Apoptosis in U937 Cells  Biosci Biotechnol Biochem  2000  64 (7), 1422-9  PubMed ID: 10945259
3753  Murakami K, Nomiyama H, Miura R, Follens A, Fiten P, Van Coillie E, Van Damme J, Opdenakker G.  Structural and functional analysis of the promoter region of the human MCP-3 gene: transactivation of expression by novel recognition sequences adjacent to the transcription initiation site.  DNA Cell Biol  1997  16(2):173-83  PubMed ID: 9052738
1855  Hashimoto E, Kage K, Ogita T, Nakaoka T, Matsuoka R, Kira Y  Adenosine as an endogenous mediator of hypoxia for induction of vascular endothelial growth factor mRNA in U-937 cells.  Biochem Biophys Res Commun  1994  204:318-24  PubMed ID: 7945378
1500  Honma M, Kataoka E, Ohnishi K, Ohno T, Takeuchi M, Nomura N, Mizusawa H  A new DNA profiling system for cell line identification for use in cell banks in Japan.  In Vitro Cell Dev Biol  1992  28A:24-8  PubMed ID: 1730567
14353  Pang L, Han S, Jiao Y, Jiang S, He X, Li P.  Bu Fei Decoction attenuates the tumor associated macrophage stimulated proliferation, migration, invasion and immunosuppression of non-small cell lung cancer, partially via IL-10 and PD-L1 regulation  Int J Oncol  2017  Jul;51(1):25-38  PubMed ID: 28534943



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