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Cell No. : Cell Name
TDC0001 : PDT-LUAD#5  update : 2025/06/12
CommentTumor organoid.
Comment from the depositorA patient derived lung cancer organoid harboring BRAF^(G469A).
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior consent on use of it from the DEVELOPER and DEPOSITOR. The RECIPIENT shall conclude an MTA with the depositor. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (NPJ Precis Oncol. 2021 5(1):29) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0075.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information IPR Kawasaki Medical School
Depositor FUKAZAWA, Takuya
Originator FUKAZAWA, Takuya
Year of deposit 2022
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65
Tissue lung
Disease name left lung cancer
Tumor pulmonary adenocarcinoma, pT1cN2M0, pStage ⅢA
Metastatic ability Unknown
Classification cancer
Recombinant non-recombinant
Year of origin 2018
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type 3D culture in matrigel three-dimensional (3D) culture
Culture medium Sachs’s AO medium (Sachs, N. et al. EMBO J. 2019 38(4):e100300) Sachs’s AO medium
Antibiotics Free
Passage method TrypLE Express
Designated culture dish 24-well plate or 48-well plate 24-well plate
Culture information Passage ratio 1:4 to 1:8 1 : 3-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week Subculture : once/7-10 days, Medium Renewal : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1 CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Others Combination treatment of trametinib and erlotinib significantly suppressed the viability of this organoid.
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
22961  Yokota E, Iwai M, Yukawa T, Naomoto Y, Haisa M, Monobe Y, Takigawa N, Fukazawa T, Yamatsuji T.   Patient-derived tumoroid models of pulmonary large-cell neuroendocrine carcinoma: a promising tool for personalized medicine and developing novel therapeutic strategies  Cancer Lett  2024  588:216816  PubMed ID: 38499265   DOI: 10.1016/j.canlet.2024.216816
22963  Yokota E, Iwai M, Ishida Y, Yukawa T, Matsubara M, Naomoto Y, Fujiwara H, Monobe Y, Haisa M, Takigawa N, Fukazawa T, Yamatsuji T.   Transforming tumoroids derived from ALK-positive pulmonary adenocarcinoma to squamous cell carcinoma in vivo  Hum Cell  2024  37(4):1132-1140  PubMed ID: 38829559   DOI: 10.1007/s13577-024-01085-8
17019  Yokota E, Iwai M, Yukawa T, Yoshida M, Naomoto Y, Haisa M, Monobe Y, Takigawa N, Guo M, Maeda Y, Fukazawa T, Yamatsuji T.  Clinical application of a lung cancer organoid (tumoroid) culture system  NPJ Precis Oncol  2021  5(1):29  PubMed ID: 33846488   DOI: 10.1038/s41698-021-00166-3
17020  Sachs N, Papaspyropoulos A, Zomer-van Ommen DD, Heo I, Böttinger L, Klay D, Weeber F, Huelsz-Prince G, Iakobachvili N, Amatngalim GD, de Ligt J, van Hoeck A, Proost N, Viveen MC, Lyubimova A, Teeven L, Derakhshan S, Korving J, Begthel H, Dekkers JF, Kumawat K, Ramos E, van Oosterhout MF, Offerhaus GJ, Wiener DJ, Olimpio EP, Dijkstra KK, Smit EF, van der Linden M, Jaksani S, van de Ven M, Jonkers J, Rios AC, Voest EE, van Moorsel CH, van der Ent CK, Cuppen E, van Oudenaarden A, Coenjaerts FE, Meyaard L, Bont LJ, Peters PJ, Tans SJ, van Zon JS, Boj SF, Vries RG, Beekman JM, Clevers H.  Long-termLong-term expanding human airway organoids for disease modeling expanding human airway organoids for disease modeling  EMBO J  2019  38(4):e100300  PubMed ID: 30643021   DOI: 10.15252/embj.2018100300

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