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Cell No. : Cell Name
RCB5988 : AK23 update : 2025/01/17 |
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| Comment | ||||
| Comment from the depositor | This clone produces pathogenic anti-mouse desmoglein 3 (Dsg3) IgG antibody that induces acantholysis in vivo. | |||
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (J Immunol 2003 170(4):2170-8) designated by the DEPOSITOR is requested. 2) If the RECIPIENT of BIOLOGICAL RESOURCE, a non-profit institution, uses the RESOURCE for non-profit purposes, the RECIPIENT must obtain written consent from the DEPOSITOR to provide the RESOURCE. 3) If the RECIPIENT of BIOLOGICAL RESOURCE intends to use the RESOURCE for commercial purposes, the RECIPIENT must consult with the DEPOSITOR and obtain written consent on the use of it from the DEPOSITOR. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | IPR | Keio University School of Medicine (Japan) | ||
| Depositor | AMAGAI, Masayuki | |||
| Originator | AMAGAI, Masayuki | |||
| Year of deposit | 2024 | |||
| Cloning (depositor) | Yes | |||
| _Date (depositor) | limiting dilution | |||
| Animal | _mouse x mouse < Hybrid | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Product | anti-mouse Dsg3 monoclonal antibody | |||
| Contact inhibition | No | |||
| Tumorigenesis | Yes | hybridoma | Fusion_cell_1 name | splenocyte |
| Fusion_cell_1 strain | Dsg3–/– mouse : 129/SV(H-2 b) x C57BL/6J(H-2 b) | |||
| Fusion_cell_1 gender | Unknown | |||
| Fusion_cell_2 name | P3 mouse myeloma cell | |||
| Fusion_cell_2 gender | Unknown | Fusion method | PEG 4000,ELISA with the recombinant mouse Dsg3 protein | Antibody name | AK23 |
| Subclass | mouse IgG1κ | |||
| Immunogen | recombinant mouse Dsg3 protein | Antigen_name | mDsg3 (130 kDa), hDsg3 (130 kDa) | |
| Antigen_character | culture supernatant | |||
| Antigen_epitope | V3, K7, P8, D59 residues of mDsg3 (AK23 recognizes comformational epitopes) | Antigen_positive | stratified squamous epithelia | |
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture method | General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10% FBS | RPMI1640 + 10% FBS | ||
| Antibiotics | Free | Free | Passage method | dilution | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | Subculture : 2-3 times/week | ||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | CELLBANKER | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgG1(Kappa) | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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