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Cell No. : Cell Name
RCB5982 : HCT116 CERT1 KO/mVenus-CERT dupAA  update : 2024/07/18
CommentCurrently not ready.
Comment from the depositorThe parental HCT116 CERT1 KO is a CERT1-disrupted derivative of the HCT 116 cell line, derived from human colon cancer. HCT116 CERT1 KO/mVenus-CERT dupAA cells represent a batch of HCT116 CERT1 KO-derived cells that stably express mVenus-fused human CERT dupAA-frameshift mutant using the retroviral vector pMXs-IB. This batch consists of a mixture of blasticidin S-resistant cells that were not cloned.
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Expected time Upon a request we will start preparation. Contact us: cellkitaku.brc@riken.jp
IPR National Institute of Infectious Diseases (Japan)
Depositor HANADA, Kentaro
Reference information Reference 2
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Reference
21780  Tamura N, Sakai S, Martorell L, Colomé R, Mizuike A, Goto A, Ortigoza-Escobar JD, Hanada K.  Intellectual disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution.  J Biol Chem  2021  101338  PubMed ID: 34688657   DOI: 10.1016/j.jbc.2021.101338
21779  Murakami H, Tamura N, Enomoto Y, Shimasaki K, Kurosawa K, Hanada K.  Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT.  PLoS One  2020  15(12):e0243980  PubMed ID: 33347465   DOI: 10.1371/journal.pone.0243980

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