Cell No. : Cell Name RCB5978 : HCT116 CERT1 KO/mVenus-CERT WT  update : 2025/01/16
Comment			Currently not ready.
Comment from the depositor			The parental HCT116 CERT1 KO is a CERT1-disrupted derivative of the HCT 116 cell line, derived from human colon cancer. HCT116 CERT1 KO/mVenus-CERT WT cells represent a batch of HCT116 CERT1 KO-derived cells that stably express mVenus-fused human CERT (wild-type) using the retroviral vector pMXs-IB. This batch consists of a mixture of blasticidin S-resistant cells that were not cloned.
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Remarks			CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
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	Basic information	Expected time	Upon a request we will start preparation. Contact us: cellkitaku.brc@riken.jp
		IPR	National Institute of Infectious Diseases (Japan)
		Depositor	HANADA, Kentaro
Reference information		Reference	2
		User's Publication	0

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Reference

21780  Tamura N, Sakai S, Martorell L, Colomé R, Mizuike A, Goto A, Ortigoza-Escobar JD, Hanada K.  Intellectual disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution.  J Biol Chem  2021  101338  PubMed ID: 34688657   DOI: 10.1016/j.jbc.2021.101338

21779  Murakami H, Tamura N, Enomoto Y, Shimasaki K, Kurosawa K, Hanada K.  Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT.  PLoS One  2020  15(12):e0243980  PubMed ID: 33347465   DOI: 10.1371/journal.pone.0243980

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