Cell No. : Cell Name
RCB5965 : WIPI1 KO HEK293T
update : 2025/01/17
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| Comment | |
| Comment from the depositor | HEK293T in which WIPI1 gene was deleted using CRISPR/Cas9-mediated genome editing. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (Hum Mol Genet 2023 32(16):2623-2637) designated by the DEPOSITOR is requested. |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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IPR |
The University of Tokyo (Japan)
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| Depositor |
MIZUSHIMA, Noboru
|
| Originator |
MIZUSHIMA, Noboru
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| Year of deposit |
2023
|
| Original cell |
293T
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Age at sampling |
embryo
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| Tissue |
kidney
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| Classification |
transformed
|
| Recombinant |
recombinant
|
| Genome Editing |
WIPI1 gene
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| Exogene |
SV40 large T antigen
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM（WAKO＃043-30085）+10％ FBS
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DMEM (high glucose) + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
0.05% Trypsin/EDTA (37 ℃, 5 min.)
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0.05% Trypsin + 0.02% EDTA
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| Designated culture dish |
6cm dish (353004, FALCON)
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Culture information
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Passage cell No |
2x10 5 cells / 6 cm dish
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2-8x10 5 cells/60mm dish
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| SC frequency |
Subculture : 2-3 times/week
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Subculture : 2-3 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
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| Freeze medium |
CellBanker (CB011, TaKaRa)
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Mycoplasma |
(-)
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| Animal PCR |
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OK
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| STR(human) |
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OK
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| Reference information |
Reference |
1
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| User's Publication |
0
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