Cell No. : Cell Name RCB5955 : HeLa/mClover3-mAID-Lem2  update : 2025/01/16
Comment			Currently not ready.
Comment from the depositor			A cell line in which the Lem2 gene in the genomic DNA is replaced with the mClover3-mAID-Lem2 gene using HeLa cells as parent cells by genome editing technology using CRISPR/Cas9. All genomic Lem2 genes have been replaced with the mClover3-mAID-Lem2 sequence. The addition of 0.5 mM indole-3-acetic acid (IAA) to the cell culture medium can degrade and eliminate intracellular Lem2 in 30-60 minutes. The degree of degradation can be confirmed by fluorescence microscopy for mClover or western blotting.
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Remarks			CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
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	Basic information	Expected time	Upon a request we will start preparation. Contact us: cellkitaku.brc@riken.jp
		IPR	Osaka University (Japan)
		Depositor	HARAGUCHI, Tokuko
		Originator	HARAGUCHI, Tokuko
Reference information		Reference	2
		User's Publication	0

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Reference

21793  Haraguchi T, Koujin T, Shindo T, Bilir Ş, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y.  Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase  Commun Biol  2022  5(1):78  PubMed ID: 35058555   DOI: 10.1038/s42003-022-03021-8

150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265

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