Cell No. : Cell Name
RCB5943 : LM8/luc
update : 2025/04/17
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| Comment | |
| Comment from the depositor | High lung metastasis cell line (isolated from C3H mouse Dan osteosarcoma cell line) expressing firefly luciferase in an NF-kB activity-dependent manner. The expression level of Fluc is not sufficient to detect fewer than 10 6 cells. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (Cancer Sci 2014 105(5):553-559, Cancer Sci 2018 109(9):2746-2756) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
Tokyo Institute of Technology (Scinece Tokyo) (Japan)
|
| Depositor |
KIZAKA=KONDOH, Shinae
|
| Originator |
KIZAKA=KONDOH, Shinae
|
| Year of deposit |
2023
|
| Original cell |
LM8
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Tissue |
bone
|
| Disease name |
osteosarcoma
|
| Recombinant |
recombinant
|
| Exogene |
firefly luciferase gene
|
| Vector |
pEF6/Myc-HisB
|
| Recombinant virus |
No
|
| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
5% FCS-DMEM
|
DMEM (high glucose) + 5% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% trypsin
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Culture information
|
Passage ratio |
|
1 : 10-20 split
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| SC frequency |
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Subculture : 2-3 times/week
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| Temperature |
|
37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
BAMBANKER (GC LYMPHOTEC)
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CELLBANKER 1
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
0
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| Reference |
|
17491
Pongsuchart M, Kuchimaru T, Yonezawa S, Tran DTP, Kha NT, Hoang NTH, Kadonosono T, Kizaka-Kondoh S.
Novel lymphoid enhancer-binding factor 1-cytoglobin axis promotes extravasation of osteosarcoma cells into the lungs
Cancer Sci
2018
109(9):2746-2756
PubMed ID: 29927026
DOI: 10.1111/cas.13702
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17490
Kuchimaru T, Hoshino T, Aikawa T, Yasuda H, Kobayashi T, Kadonosono T, Kizaka-Kondoh S.
Bone resorption facilitates osteoblastic bone metastatic colonization by cooperation of insulin-like growth factor and hypoxia
Cancer Sci
2014
105(5):553-9.
PubMed ID: 24597654
DOI: 10.1111/cas.12391
|
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