Cell No. : Cell Name
RCB5942 : HEK293/κB-Fluc
update : 2025/01/24
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| Comment | |
| Comment from the depositor | A human embryonic kidney cell line that expresses the firefly luciferase gene in an NF-kB activity-dependent manner. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (J Am Chem Soc 2014 136(48) : 16902-8) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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IPR |
Tokyo Institute of Technology (Scinece Tokyo) (Japan)
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| Depositor |
KIZAKA=KONDOH, Shinae
|
| Originator |
KIZAKA=KONDOH, Shinae
|
| Year of deposit |
2023
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| Original cell |
HEK293
|
| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Age at sampling |
embryo
|
| Tissue |
kidney
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| Classification |
transformed
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| Recombinant |
recombinant
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| Exogene |
firefly luciferase gene
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| Vector |
pGL4/κB-luc2P
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| Memo_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
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Lifespan |
infinite
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
5% FCS-DMEM
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DMEM (high glucose) + 5% FBS
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| Antibiotics |
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Free
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Passage method |
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0.25% trypsin
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Culture information
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Passage ratio |
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1 : 8-10 split
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| SC frequency |
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Subculture : 1-2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
BAMBANKER (GC LYMPHOTEC)
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| STR(human) |
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OK
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| Reference information |
Reference |
1
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| User's Publication |
0
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