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Cell No. : Cell Name
RCB5941 : LLC/mKO2-Rluc8.6 update : 2024/11/11 |
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| Comment | ||||
| Comment from the depositor | Mouse lung cancer cell line stably retaining Kusabira Orange2 (mKO2) fluorescent protein and firefly luciferase gene. | |||
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Nat Commun 2018 9(1):2981) designated by the DEPOSITOR is requested. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the mKO2-Developer (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the mKO2-Developer is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the mKO2-Developer prior to MTA. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | IPR | Tokyo Institute of Technology (Japan) | ||
| Depositor | KIZAKA-KONDOU, Shinae | |||
| Originator | KIZAKA-KONDOU, Shinae | |||
| Original cell | LLC | |||
| Animal | _mouse < Mammals | |||
| Strain name | C57BL | |||
| Genus | Mus | |||
| Species | musculus, | |||
| Tissue | Lung | |||
| Disease name | Lewis Lung Carcinoma | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | Kusabira Orange2 (mKO2), firefly luciferase gene | |||
| Vector | pT2/SVNeo | Lifespan | infinite | |
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | 5% FCS-DMEM | DMEM (high glucose) + 5% FBS | ||
| Antibiotics | Free | Passage method | 0.25% trypsin + 0.02%EDTA | Culture information | Passage ratio | 1 : 4-8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | BAMBANKER (GC LYMPHOTEC) | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| SSLP(mouse) | OK | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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