Cell No. : Cell Name
RCB5939 : 4T1/luc
update : 2024/11/11
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| Comment | |
| Comment from the depositor | A murine breast cancer cell line (derived from the mammary gland tissue of a mouse BALB/c strain) that stably retains the firefly luciferase gene. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Nat Commun 2018 9(1):2981) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
Tokyo Institute of Technology (Japan)
|
| Depositor |
KIZAKA-KONDOU, Shinae
|
| Originator |
KIZAKA-KONDOU, Shinae
|
| Year of deposit |
2023
|
| Original cell |
4T1
|
| Animal |
_mouse
< Mammals
|
| Strain name |
BALB/cfC3H
|
| Genus |
Mus
|
| Species |
musculus
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| Tissue |
Mammary gland
|
| Disease name |
breast cancer
|
| Classification |
cancer
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| Recombinant |
recombinant
|
| Exogene |
firefly luciferase gene
|
| Vector |
pEF6/Myc-HisB
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|
Lifespan |
infinite
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
10% FCS-RPMI
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RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% trypsin + 0.02%EDTA
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Culture information
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Passage ratio |
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1 : 8-10 split
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| SC frequency |
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Subculture : 2-3 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
BAMBANKER (GC LYMPHOTEC)
|
CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Mycoplasma |
(-)
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| Animal PCR |
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OK
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| SSLP(mouse) |
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OK
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| Reference information |
Reference |
1
|
| User's Publication |
0
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