Cell No. : Cell Name
RCB5936 : MDA-MB-231/luc
update : 2024/11/19
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| Comment | |
| Comment from the depositor | A human breast cancer cell line that stably retains the firefly luciferase gene. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (Nat Commun 2016 7:11856, Nat Commun 2018 9(1):2981) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
Tokyo Institute of Technology (Japan)
|
| Depositor |
KIZAKA-KONDOU, Shinae
|
| Originator |
KIZAKA-KONDOU, Shinae
|
| Year of deposit |
2023
|
| Original cell |
MDA-MB-231
|
| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Female
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| Age at sampling |
51 years
|
| Tissue |
mammary gland
|
| Disease name |
breast cancer
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
firefly luciferase gene
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| Vector |
pEF6/Myc-HisB
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|
Lifespan |
infinite
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
|
| Culture medium |
5% FCS-DMEM
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DMEM (high glucose) + 5% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% trypsin
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Culture information
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Passage ratio |
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1 : 8-10 split
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| SC frequency |
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Subculture : 1-2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
BAMBANKER (GC LYMPHOTEC)
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Mycoplasma |
(-)
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| Animal PCR |
|
OK
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| STR(human) |
|
OK
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
|
17488
Kuchimaru T, Kataoka N, Nakagawa K, Isozaki T, Miyabara H, Minegishi M, Kadonosono T, Kizaka-Kondoh S.
A reliable murine model of bone metastasis by injecting cancer cells through caudal arteries
Nat Commun
2018
9(1):2981
PubMed ID: 30061695
DOI: 10.1038/s41467-018-05366-3
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17487
Kuchimaru T, Iwano S, Kiyama M, Mitsumata S, Kadonosono T, Niwa H, Maki S, Kizaka-Kondoh S.
A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging
Nat Commun
2016
7:11856
PubMed ID: 27297211
DOI: 10.1038/ncomms11856
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