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Cell No. : Cell Name
RCB5932 : 63B2 update : 2024/02/28 |
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| Comment | ||||
| Comment from the depositor | Anti-ETNPPL antibody (for immunohistochemistry) producing hybridoma. | |||
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature((Frontiers in Cellular Neuroscience 2023 30 January) designated by the DEPOSITOR is requested. 2) The RECIPIENTwhich wants to use the BIOLOGIAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka Unlversity. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | TSUJIOKA, Hiroshi | ||
| Originator | TSUJIOKA, Hiroshi | |||
| Year of deposit | 2022 | |||
| Another name | Anti-ETNPPL monoclonal antibody producing hybridoma clone 63B2 (for immunohistochemistry) | |||
| Cloning (depositor) | Yes | |||
| _Date (depositor) | 2021 | |||
| _Method (depositor) | Limiting dilution | |||
| Animal | _mouse x rat < Hybrid | Lifespan | infinite | |
| Morphology | lymphocyte-like | hybridoma | Fusion_cell_1 name | X-63 Ag8・653 |
| Fusion_cell_1 strain | Balb/c, Mus musculus | |||
| Fusion_cell_1 gender | Unknown | |||
| Fusion_cell_2 name | spleen (B cell) | |||
| Fusion_cell_2 strain | Wistar Rat, Rattus norvegicus | |||
| Fusion_cell_2 gender | Female | Fusion method | PEG, HAT selection, Limiting dilution | Antibody name | ETNPPL |
| Immunogen | His-tagged recombinant mouse ETNPPL | Antigen_name | ETNPPL | |
| Antigen_m.w. | 55.5kDa | Antigen_positive | astrocytes | |
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture medium | 10％FCS (gibco FBS REF:10270-106), TIL Media Ⅰ（Immuno-Biological Laboratories Co., Ltd., 33640）or KBM 353 (Kohjin Bio Co., Ltd.) | KBM 353 + 5mM Glutamine + 10% FBS | ||
| Culture method | ||||
| Antibiotics | Free | Passage method | dilution | |
| Designated culture dish | T25, T75 | Culture information | Passage cell No | 1-2ｘ10 5/mL | 1.0-2.5ｘ10 5 /mL |
| Passage ratio | 1 : 4-8 split | |||
| SC frequency | every 2 days | Subculture : 2-3 times/week | ||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | CELLBANKER 1 (ZNQ #CB011) | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
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