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Cell No. : Cell Name
RCB5719 : HL-Q37C-701G7  update : 2023/10/19
Comment
Comment from the depositorHeLa cells (RIKEN BRC, RCB0007) expressing QUEEN-37C, an ATP sensor fluorescent protein.
Terms and conditions1)The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a a not-for profit research. 2) For use of the BIOLOGICAL RESOURCE by a for-profit organization or by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on Terms and Conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 3) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or for- profit use based on the results from the use of the BIOLOGICAL RESOURCE.) 4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Hideyuki Yaginuma, Yasushi Okada Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C biorxiv 2021) designated by the DEPOSITOR is requested. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
approver's address
×
English
Address
Laboratory for Cell Polarity Regulation, RIKEN Center for Biosystems Dynamics Research.
6-2-3 Furue-dai, Suita-city, Osaka, 565-0874, Japan.
Dr.Okada Yasushi
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor OKADA, Yasushi
Originator YAGINUMA, Hideyuki
Year of deposit 2022
Original cell HeLa (RCB0007)
Cloning (depositor) Yes
_Method (depositor) Single Colony
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Primary focus cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene QUEEN37C/pLBS.CAG
Vector pLBS lentiviral transfer vector
Recombinant virus No
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
17399  Hideyuki Yaginuma, Yasushi Okada  Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C  biorxiv  2021      DOI: 10.1101/2021.10.08.463131

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User's Publication



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