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Cell No. : Cell Name
RCB5719 : HL-Q37C-701G7 update : 2025/01/31 |
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| Comment | ||||
| Comment from the depositor | HeLa cells (RIKEN BRC, RCB0007) expressing QUEEN-37C, an ATP sensor fluorescent protein. | |||
| Terms and conditions | 1)The availability of the BIOLOGICAL RESOURCE is restricted to a RECIPIENT of a not-for profit organization for a not-for profit research. 2) For use of the BIOLOGICAL RESOURCE by a for-profit organization or by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on Terms and Conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 3) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or for- profit use based on the results from the use of the BIOLOGICAL RESOURCE.) 4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Hideyuki Yaginuma, Yasushi Okada Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP indicator protein, QUEEN-37C biorxiv 2021) designated by the DEPOSITOR is requested. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | OKADA, Yasushi | ||
| Originator | YAGINUMA, Hideyuki | |||
| Year of deposit | 2022 | |||
| Original cell | HeLa (RCB0007) | |||
| Cloning (depositor) | Yes | |||
| _Method (depositor) | Single Colony | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Gender | Female | |||
| Age at sampling | 31 years | |||
| Tissue | cervix | |||
| Primary focus | cervical carcinoma | |||
| Classification | cancer | |||
| Recombinant | recombinant | |||
| Exogene | QUEEN37C/pLBS.CAG | |||
| Vector | pLBS lentiviral transfer vector | |||
| Recombinant virus | No | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | MEM + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | CELLBANKER 1 | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| STR(human) | OK | |||
| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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