Cell No. : Cell Name
RCB5714 : Y-MESO-61
update : 2025/01/22
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| Comment | |
| Comment from the depositor | Human pleural mesothelioma cell line. Epithelioid type. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Cancer Sci 2015 106(8):990-9) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
Aichi Cancer Center (Japan)
|
| Depositor |
SEKIDO, Yoshitaka
|
| Originator |
SEKIDO, Yoshitaka
|
| Year of deposit |
2022
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
65 years
|
| Tissue |
pleural effusion
|
| Primary focus |
pleural mesothelioma
|
| Disease name |
pleural mesothelioma
|
| Tumor |
pleural mesothelioma (pT2N2M0, Stage IIIB)
|
| Metastatic ability |
Unknown
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
| Year of origin |
2011
|
|
Lifespan |
infinite
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
RPMI1640 + 10% FBS
|
RPMI1640 + 10% FBS
|
| Antibiotics |
1% antimicrobial
|
Free
|
|
Passage method |
0.25w/v% Trypsin + 1mmol/l EDTA (FUJIFILM 209-16941)
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
|
Passage ratio |
1 : 5-8 split
|
1 : 5 split
|
| SC frequency |
Subculture : 1-2 times/week
|
Subculture : 1-2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
(-)
|
|
| Virus (HBV) |
(-)
|
|
| Virus (HCV) |
(-)
|
|
| Virus |
|
OK
|
|
deposit info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
10,11
|
11
|
11,13
|
10,11
|
17,19
|
7,8
|
X,Y
|
9,11
|
10,12
|
|
|
lot info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
10,11
|
11
|
11,13
|
10,11
|
17,19
|
7,8
|
X,Y
|
9,11
|
10,12
|
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
16027
Matsushita A, Sato T, Mukai S, Fujishita T, Mishiro-Sato E, Okuda M, Aoki M, Hasegawa Y, Sekido Y.
TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation
Oncogene
2018
38(11):1966-1978
PubMed ID: 30401981
DOI: 10.1038/s41388-018-0417-7
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16468
Hakiri S, Osada H, Ishiguro F, Murakami H, Murakami-Tonami Y, Yokoi K, Sekido Y.
Functional differences between wild-type and mutant-type BRCA1-associated protein 1 tumor suppressor against malignant mesothelioma cells
Cancer Sci
2015
106(8):990-9.
PubMed ID: 26011428
DOI: 10.1111/cas.12698
|
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