Cell No. : Cell Name RCB5712 : Y-MESO-45  update : 2025/01/24
Comment
Comment from the depositor			Human pleural mesothelioma cell line.
Terms and conditions			In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Oncogene 2012 31(49):5117-22) designated by the DEPOSITOR is requested.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	IPR	Aichi Cancer Center (Japan)
		Depositor	SEKIDO, Yoshitaka
		Originator	SEKIDO, Yoshitaka
		Year of deposit	2022
		Cloning (depositor)	No
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	49 years
		Tissue	pleural effusion
		Primary focus	pleural mesothelioma
		Disease name	pleural mesothelioma
		Tumor	pleural mesothelioma
		Classification	cancer
		Recombinant	non-recombinant
		Year of origin	2009
		Lifespan	infinite
			deposit info	lot info
		Medium	Medium List
		Culture type	Adherent cells	Adherent cells
		Culture medium	RPMI1640 + 10% FBS	RPMI1640 + 10% FBS
		Antibiotics	1% antimicrobial	Free
		Passage method	0.25w/v% Trypsin + 1mmol/l EDTA (FUJIFILM 209-16941)	0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
	Culture information	Passage ratio	1 : 5-8 split	1 : 5-8 split
		SC frequency	Subculture : 1-2 times/week	Subculture : 1-2 times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	CELLBANKER	CELLBANKER 1
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Mycoplasma	(-)
		Animal PCR		OK
		Virus (HIV)	(-)
		Virus (HBV)	(-)
		Virus (HCV)	(-)
		STR(human)		OK
		deposit info	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 10,14 10 8,9 12 14 9 X 11 12
		lot info	D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO 10,14 10 8,9 12 14 9 X 11 12
Reference information		Reference	5
		User's Publication	0

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Reference

16027  Matsushita A, Sato T, Mukai S, Fujishita T, Mishiro-Sato E, Okuda M, Aoki M, Hasegawa Y, Sekido Y.  TAZ activation by Hippo pathway dysregulation induces cytokine gene expression and promotes mesothelial cell transformation  Oncogene  2018  38(11):1966-1978  PubMed ID: 30401981   DOI: 10.1038/s41388-018-0417-7

16477  Kato T, Sato T, Yokoi K, Sekido Y.  E-cadherin expression is correlated with focal adhesion kinase inhibitor resistance in Merlin-negative malignant mesothelioma cells  Oncogene  2017  36(39):5522-5531  PubMed ID: 28553954   DOI: 10.1038/onc.2017.147

16468  Hakiri S, Osada H, Ishiguro F, Murakami H, Murakami-Tonami Y, Yokoi K, Sekido Y.  Functional differences between wild-type and mutant-type BRCA1-associated protein 1 tumor suppressor against malignant mesothelioma cells  Cancer Sci  2015  106(8):990-9.  PubMed ID: 26011428   DOI: 10.1111/cas.12698

16469  Tanaka I, Osada H, Fujii M, Fukatsu A, Hida T, Horio Y, Kondo Y, Sato A, Hasegawa Y, Tsujimura T, Sekido Y.  LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via Hippo signaling cascade  Oncogene  2015  34(1):73-83  PubMed ID: 24336325   DOI: 10.1038/onc.2013.528

16471  Mizuno T, Murakami H, Fujii M, Ishiguro F, Tanaka I, Kondo Y, Akatsuka S, Toyokuni S, Yokoi K, Osada H, Sekido Y.  YAP induces malignant mesothelioma cell proliferation by upregulating transcription of cell cycle-promoting genes  Oncogene  2012  31(49):5117-22  PubMed ID: 22286761   DOI: 10.1038/onc.2012.5

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