Cell No. : Cell Name
RCB5711 : Y-MESO-43
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Human malignant pleural mesothelioma cell line, which was initially diagnosed biphasic type. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Oncogene 2015 34(1):73-83) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
Aichi Cancer Center Research Institute (Japan)
|
| Depositor |
SEKIDO, Yoshitaka
|
| Originator |
SEKIDO, Yoshitaka
|
| Year of deposit |
2022
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
50 years
|
| Tissue |
pleural effusion
|
| Primary focus |
malignant pleural mesothelioma
|
| Disease name |
malignant pleural mesothelioma
|
| Tumor |
malignant pleural mesothelioma
|
| Metastatic ability |
Unknown
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
| Year of origin |
2008
|
|
Lifespan |
infinite
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
RPMI1640 + 10% FBS
|
RPMI1640 + 10% FBS
|
| Antibiotics |
1% antimicrobial
|
Free
|
|
Passage method |
0.25w/v% Trypsin + 1mmol/L EDTA (FUJIFILM Solution 209-16941)
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
|
Passage ratio |
1/5-1/8
|
1 : 5-8 split
|
| SC frequency |
Subculture : 1-2 times/week
|
Subculture : 1-2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
(-)
|
|
| Virus (HBV) |
(-)
|
|
| Virus (HCV) |
(-)
|
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
16469
Tanaka I, Osada H, Fujii M, Fukatsu A, Hida T, Horio Y, Kondo Y, Sato A, Hasegawa Y, Tsujimura T, Sekido Y.
LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via Hippo signaling cascade
Oncogene
2015
34(1):73-83
PubMed ID: 24336325
DOI: 10.1038/onc.2013.528
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16471
Mizuno T, Murakami H, Fujii M, Ishiguro F, Tanaka I, Kondo Y, Akatsuka S, Toyokuni S, Yokoi K, Osada H, Sekido Y.
YAP induces malignant mesothelioma cell proliferation by upregulating transcription of cell cycle-promoting genes
Oncogene
2012
31(49):5117-22
PubMed ID: 22286761
DOI: 10.1038/onc.2012.5
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