Cell No. : Cell Name
RCB5707 : Parental HEK293T of TMEM41B-KO and VMP1-KO
update : 2025/08/19
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| Comment | |
| Comment from the depositor | Parental cells of TMEM41B-KO and VMP1-KO HEK293T cells. |
| Terms and conditions | 1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (J Cell Biol 2018 217(11):3817-3828) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
MIZUSHIMA, Noboru
|
| Year of deposit |
2022
|
| Animal |
_human
< Mammals
|
| Strain name |
Homo
|
| Genus |
sapiens
|
| Age at sampling |
embryo
|
| Tissue |
kidney
|
| Classification |
transformed
|
| Exogene |
SV40 large T antigen
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM（WAKO＃043-30085）+10％ FBS
|
DMEM (high glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
0.05% Trypsin/EDTA (37 ℃, 5 min.)
|
0.05% Trypsin + 0.02% EDTA
|
| Designated culture dish |
6cm dish (353004, FALCON)
|
|
Culture information
|
Passage cell No |
2x10 5 cells / 6cm dish
|
2x10 5 cells/60mm dish
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| SC frequency |
Subculture : 2-3 times/week
|
Subculture : 2-3 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CellBanker (CB011, TaKaRa)
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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