Cell No. : Cell Name
RCB5706 : SS-GFP-RFP-KDEL HeLa
update : 2024/11/11
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| Comment | |
| Comment from the depositor | HeLa stably expressing GFP-RFP-KDEL. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell 2019 74(5):909-921) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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IPR |
The University of Tokyo (Japan)
|
| Depositor |
MIZUSHIMA, Noboru
|
| Originator |
MIZUSHIMA, Noboru
|
| Year of deposit |
2022
|
| Original cell |
ATGs Parental HeLa (RCB5692)
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
SS-GFP-RFP-KDEL
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
Adherent cells
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Adherent cells
|
| Culture medium |
DMEM（WAKO＃043-30085）+ 10％FBS
|
DMEM(high glucose) + 10%FBS
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| Antibiotics |
|
Free
|
|
Passage method |
0.05% Trypsin/EDTA
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0.05% trypsin + 0.02%EDTA
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| Designated culture dish |
6cm dish (353004, FALCON)
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Culture information
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Passage cell No |
2x10 5 cells / 6cm dish
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2x10 5 cells/60mm dish
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| SC frequency |
Subculture : 2-3 times/week
|
Subculture : 2-3 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CellBanker (CB011, TaKaRa)
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CELLBANKER 1
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Mycoplasma |
(-)
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|
| Animal PCR |
|
OK
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| STR(human) |
|
OK
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| Reference information |
Reference |
1
|
| User's Publication |
0
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