Cell No. : Cell Name
RCB5699 : ATG2A/B double KO HeLa
update : 2024/11/11
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| Comment | |
| Comment from the depositor | HeLa in which ATG2A/B genes were deleted using CRISPR/Cas9-mediated genome editing. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (FEBS Lett 2017 591(23):3819-3830) designated by the DEPOSITOR is required. |
| Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0065.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
MIZUSHIMA, Noboru
|
| Originator |
MIZUSHIMA, Noboru
|
| Year of deposit |
2022
|
| Original cell |
ATGs Parental HeLa (RCB5692)
|
| Animal |
_human
< Mammals
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| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
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| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
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| Classification |
cancer
|
| Recombinant |
recombinant
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
DMEM（WAKO＃043-30085）+ 10％FBS
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DMEM(high glucose) + 10%FBS
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| Antibiotics |
|
Free
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|
Passage method |
0.05% Trypsin/EDTA
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0.05% trypsin + 0.02%EDTA
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| Designated culture dish |
6cm dish (353004, FALCON)
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Culture information
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Passage cell No |
2x10 5 cells / 6cm dish
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2x10 5 cells/60mm dish
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| SC frequency |
Subculture : 2-3 times/week
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Subculture : 2-3 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CellBanker (CB011, TaKaRa)
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Mycoplasma |
(-)
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| Animal PCR |
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OK
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| STR(human) |
|
OK
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| Reference information |
Reference |
1
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| User's Publication |
0
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