Cell No. : Cell Name
RCB5692 : ATGs Parental HeLa
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Autophagy related gene knockout HeLa. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2016 354(6315):1036-1041, FEBS Lett 2017 591(23):3819-3830, Mol Cell 2019 74(5):909-921, J Cell Sci 2021 34(8):jcs255877) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
MIZUSHIMA, Noboru
|
| Originator |
MIZUSHIMA, Noboru
|
| Year of deposit |
2022
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
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| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM（WAKO＃043-30085）+ 10％FBS
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DMEM(high glucose) + 10%FBS
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| Antibiotics |
|
Free
|
|
Passage method |
0.05% Trypsin/EDTA
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0.05% trypsin + 0.02%EDTA
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| Designated culture dish |
6cm dish (353004, FALCON)
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Culture information
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Passage cell No |
2x10 5 cells / 6cm dish
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2x10 5 cells/60mm dish
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| SC frequency |
Subculture : 2-3 times/week
|
Subculture : 2-3 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CellBanker (CB011, TaKaRa)
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CELLBANKER 1
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Mycoplasma |
(-)
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|
| Animal PCR |
|
OK
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| STR(human) |
|
OK
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| Reference information |
Reference |
4
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| User's Publication |
0
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| Reference |
|
17346
Okawa F, Hama Y, Zhang S, Morishita H, Yamamoto H, Levine TP, Mizushima N.
Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1
J Cell Sci
2021
34(8):jcs255877.
PubMed ID: 33771928
DOI: 10.1242/jcs.255877
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|
17347
Chino H, Hatta T, Natsume T, Mizushima N.
Intrinsically Disordered Protein TEX264 Mediates ER-phagy
Mol Cell
2019
74(5):909-921
PubMed ID: 31006538
DOI: 10.1016/j.molcel.2019.03.033
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17344
Tamura N, Nishimura T, Sakamaki Y, Koyama-Honda I, Yamamoto H, Mizushima N.
Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets
FEBS Lett
2017
591(23):3819-3830
PubMed ID: 29113029
DOI: 10.1002/1873-3468.12901
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17345
Tsuboyama K, Koyama-Honda I, Sakamaki Y, Koike M, Morishita H, Mizushima N
The ATG conjugation systems are important for degradation of the inner autophagosomal membrane
Science
2016
354(6315):1036-1041
PubMed ID: 27885029
DOI: 10.1126/science.aaf6136
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