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Cell No. : Cell Name
RCB5687 : MDS-LGF update : 2025/09/17 |
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| Comment | ||||
| Comment from the depositor | MDS-LGF cell line is a blastic subline originated from MDS-L. (MDS-L cell line is a blastic subline derived from MDS92 which was established from the bone marrow of a patient with MDS.) MDS-LGF cells proliferate without IL-3 and have lost maturing capacity. | |||
| Terms and conditions | 1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior consent on use of it from the DEVELOPER and DEPOSITOR. The RECIPIENT shall conclude a MTA with the depositor. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Leukemia 2018 32(8):1846-1850) designated by the DEPOSITOR is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | IPR | Kawasaki University of Medical Welfare (Japan) | ||
| Depositor | TOHYAMA, Kaoru | |||
| Originator | TOHYAMA, Kaoru | |||
| Year of deposit | 2022 | |||
| Cloning (depositor) | No | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Gender | Male | |||
| Age at sampling | 54 years | |||
| Tissue | bone marrow | |||
| Primary focus | bone marrow | |||
| Disease name | myelodysplastic syndrome (MDS) | |||
| Tumor | MDS-EB1 stage from MDS-RS | |||
| Classification | cancer | |||
| Recombinant | non-recombinant | |||
| Year of origin | 1991 | |||
| Memo_1 | splice donor site mutation of TP53 (c.672+1G>A; homozygous; COSM6906) NRAS (G12A) mutation (heterozygous; COSM565) CEBPA (Q311stop) mutation (heterozygous; COSM29221) HIST1H3C : wild type in all the cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Differentiation | Whole cells retain immature blastic features and differentiation-induction is successful at present. | |||
| IL-3-independent, but the addition of IL-3 prmotes cell growth. | ||||
| Contact inhibition | No | |||
| Tumorigenesis | No | |||
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture method |
General Guidelines for Culturing Suspension Cells  浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium | See : How_to_culture_MDS-LGF.pdf | RPMI1640 + 10% FBS + 50μM 2-mercaptoethanol + (thawing : 20ng/ml hIL-3) | ||
| Antibiotics | Free | Passage method | See : How_to_culture_MDS-LGF.pdf | dilution | Culture information | Passage cell No | 1-2x10 5 cells/ml |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | 10%DMSO / 40%FCS / 50%RPMI1640medium | 50% RPMI1640 medium + 40%FBS + 10% DMSO | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Virus (HIV) | Undetected | |||
| Virus (HTLV-1) | (-) | |||
| Virus (EBV) | (-) | |||
| Chromosome mode | As a reference, the main karyotype of the parental MDS-L cell line is: 49, XY, +1, der(5)t(5;19), -7, +8, -12, der(13)t(7;13), der(14)t(12;14), der(15)t(15;15), -17, +19, +20, der(21)t(15;21), der(22)t(11;22). | |||
| Surface antigen | CD13, CD33:strongly positive; CD34: strongly positive; HLA-DR: positive. CD7: negative | |||
| Doubling time | 72 hr | |||
| Memo_2 | The cell growth may depend on the lot of FCS and IL-3, and the cells sometimes show poor growth. | |||
| STR(human) | OK | |||
| Relational File | deposit info | lot info | ||
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| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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How_to_culture_MDS-LGF.pdf