Cell No. : Cell Name
RCB5682 : Astrocyte(ASD)
update : 2024/11/11
|
| Comment | |
| Comment from the depositor | A cultured cell line derived from a strain of primary astrocyte cells isolated from C57BL/6x129sv brain (cerebrum). |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KAWASHIMA, Nagako
|
| Originator |
TSUJI, Daisuke
|
| Year of deposit |
2022
|
| Cloning (depositor) |
No
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6 x 129sv
|
| Genus |
Mus
|
| Species |
musculus
|
| Age at sampling |
1 day
|
| Tissue |
Brain (Cerebrum)
|
| Classification |
semi-normal
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Biochemical |
The growth rate is fast like that of cancer cells.
|
| Surface antigen (depositor) |
GFAP, GM2, GA2
|
| Contact inhibition |
No
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM (110 µg/mL sodium pyruvate) + 10% heat inactivated FBS
|
DMEM (high glucose)+ 10% FBS (heat inactivated)
|
| Antibiotics |
Penicillin (100 µg/mL), Streptomycin (50 µg/mL)
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Designated culture dish |
T-25 Flask
|
|
Culture information
|
Passage cell No |
2-3x10 5 cells/plate
|
2-3x10 5 cells/60mm dish
|
| SC frequency |
Subculture : 1-2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Saturation density |
80-90%
|
|
| Doubling time |
24
hr
|
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
16544
Kawashima N, Tsuji D, Okuda T, Itoh K, Nakayama K.
Mechanism of abnormal growth in astrocytes derived from a mouse model of GM2 gangliosidosis
J Neurochem
2009
111(4):1031-41
PubMed ID: 19765188
DOI: 10.1111/j.1471-4159.2009.06391.x
|
|
16542
Tsuji D, Kuroki A, Ishibashi Y, Itakura T, Itoh K.
Metabolic correction in microglia derived from Sandhoff disease model mice
J Neurochem
2005
94(6):1631-8
PubMed ID: 16092933
DOI: 10.1111/j.1471-4159.2005.03317.x
|
|
16543
Tsuji D, Kuroki A, Ishibashi Y, Itakura T, Kuwahara J, Yamanaka S, Itoh K.
Specific induction of macrophage inflammatory protein 1-alpha in glial cells of Sandhoff disease model mice associated with accumulation of N-acetylhexosaminyl glycoconjugates
J Neurochem
2005
92(6):1497-507
PubMed ID: 15748167
DOI: 10.1111/j.1471-4159.2005.02986.x
|
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