Cell No. : Cell Name
RCB5676 : CHK-Q
update : 2024/02/26
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| Comment | |
| Comment from the depositor | A spontaneously immortalized cell line from primary culture of kidney tissue of Chinese hamsters bred as experimental animals. |
| Terms and conditions | 1) Applies only for research purpose. If the purpose of these cells and any transformations involving these cells are for business, approval from Manufacturing Technology Association of Biologics, the providers of these cells, must be gotten beforehand. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(PLoS One 2022 17(3):e0266061) designated by the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KAMIHIRA, Masamichi
|
| Originator |
KAMIHIRA, Masamichi
|
| Year of deposit |
2022
|
| Cloning (depositor) |
Yes
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Genus |
Cricetulus
|
| Species |
griseus
|
| Gender |
Female
|
| Tissue |
kidney
|
| Classification |
semi-normal
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_YS94
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM/F-12 (DF) (FUJIFILM Wako Pure Chemical Corporation 042–30555) ＋ 10% FBS
|
DMEM/HamF12(with HEPES and Sodium Pyruvate) ＋ 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Coating dish |
Collagen Type I
|
Collagen Type I
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Culture information
|
Passage ratio |
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1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
(-)
|
(-)
|
| Animal PCR |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
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