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Cell No. : Cell Name
RCB5676 : CHK-Q  update : 2024/02/26
Comment from the depositorA spontaneously immortalized cell line from primary culture of kidney tissue of Chinese hamsters bred as experimental animals.
Terms and conditions1) Applies only for research purpose. If the purpose of these cells and any transformations involving these cells are for business, approval from Manufacturing Technology Association of Biologics, the providers of these cells, must be gotten beforehand. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(PLoS One 2022 17(3):e0266061) designated by the DEPOSITOR is requested.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor KAMIHIRA, Masamichi
Originator KAMIHIRA, Masamichi
Year of deposit 2022
Cloning (depositor) Yes
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Tissue kidney
Classification semi-normal
Recombinant non-recombinant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_YS94
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM/F-12 (DF) (FUJIFILM Wako Pure Chemical Corporation 042–30555) + 10% FBS DMEM/HamF12(with HEPES and Sodium Pyruvate) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish Collagen Type I Collagen Type I
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-) (-)
Animal PCR OK
Reference information Reference 2
User's Publication 0

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16449  Kawabe Y, Kamihira M.  Novel cell lines derived from Chinese hamster kidney tissue  PLoS One  2022  17(3):e0266061.  PubMed ID: 35358245   DOI: 10.1371/journal.pone.0266061
16480  Yusuke Sakai, Yoshihiro Kubo, Nana Shirakigawa, Yoshinori Kawabe, Masamichi Kamihira, Hiroyuki Ijima  Promotion of Cyst Formation from a Renal Stem Cell Line Using Organ-Specific Extracellular Matrix Gel Format Culture System  Gels  2022  8(5):312  PubMed ID: 35621610   DOI: 10.3390/gels8050312

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