細胞番号 : 細胞名
RCB5664 : Y-MESO-8A
update : 2024/11/11
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細胞特性(Comment:英) | RCB5664 Y-MESO-8A, RCB5665 Y-MESO-8D were derived from the same patient. |
細胞特性(日) | RCB5664 Y-MESO-8A、RCB5665 Y-MESO-8Dは同一人由来。 |
細胞特性(寄託者記述:英) | Human malignant pleural mesothelioma cell line. The primary tumor is a biphasic (predominantly sarcomatoid) tumor tissue. Subcloned from patient's pleural fluid and shows epithelioid morphology. |
細胞特性(寄託者記述:日) | ヒト悪性胸膜中皮腫細胞株。原発巣は2相型(肉腫型が主)の腫瘍組織。患者胸水よりサブクローニングされて樹立され上皮型(epithelioid)の形態を示す。 |
使用条件(英) | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Cancer Sci 2006 97:387-394) designated by the DEPOSITOR is requested. |
使用条件(日) | 研究成果の公表にあたって寄託者の指定する文献 (Cancer Sci 2006 97:387-394) を引用すること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
|
|
寄託者 |
SEKIDO, Yoshitaka
|
樹立者 |
SEKIDO, Yoshitaka
|
寄託日 |
2022
|
元の細胞 |
Y-MESO-8
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クローニング(寄託者) |
Yes
|
クローニングの時期(寄託者) |
2001
|
クローニングの方法(寄託者) |
ring cloning
|
動物種 |
_human
< Mammals
|
属名 |
Homo
|
種名 |
sapiens
|
人種 |
Japanese
|
性別 |
Male
|
齢 |
61 years
|
採取組織 |
pleural effusion
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原発組織 |
malignant pleural mesothelioma
|
病名 |
malignant pleural mesothelioma
|
腫瘍 |
malignant pleural mesothelioma (biphasic (predominantly sarcomatoid)
|
転移 |
Unknown
|
細胞分類 |
cancer
|
遺伝子改変 |
non-recombinant
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初代培養日 |
2001
|
|
細胞寿命 |
infinite
|
細胞形態 |
epithelial-like
|
Cellosaurus(Expasy) |
CVCL_5188
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
Adherent cells
|
Adherent cells
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培地 |
RPMI1640 + 10% FBS
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RPMI1640 + 10% FBS
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抗生物質 |
1% antimicrobial
|
Free
|
|
継代方法 |
0.25w/v% Trypsin + 1mmol/l EDTA(FUJIFILM 209-16941)
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
|
継代密度 |
1 : 5-8 split
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1 : 5-8 split
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継代・培地交換頻度 |
Subculture : 1-2 times/week
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Subculture : 1-2 times/week
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培養最適温度 |
37
℃
|
37
℃
|
二酸化炭素濃度 |
5
%
|
5
%
|
凍結培地 |
CELLBANKER
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CELLBANKER 1
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凍結方法 |
|
Slow freezing
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マイコプラズマ/アコレプラズマ |
|
(-)
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マイコプラズマ |
(-)
|
|
動物種PCR |
|
OK
|
ウィルス(HIV) |
(-)
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Undetected
|
ウィルス(HTLV-1) |
|
(-)
|
ウィルス(HBV) |
(-)
|
|
ウィルス(HCV) |
(-)
|
|
ウィルス(EBV) |
|
(-)
|
個体識別検査 |
|
OK
|
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寄託時情報
|
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
11,12
|
8,9
|
11
|
12
|
14,16
|
7,9
|
X,Y
|
11
|
11,12
|
|
ロット情報
|
D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
11,12
|
8,9
|
11
|
12
|
14,16
|
7,9
|
X,Y
|
11
|
11,12
|
|
文献情報 |
Reference(英) |
15件
|
Reference(日) |
0件
|
利用者成果(英) |
0件
|
利用者成果(日) |
0件
|
Reference(英) |
16452
Okazaki Y, Misawa N, Akatsuka S, Kohyama N, Sekido Y, Takahashi T, Toyokuni S.
Frequent homozygous deletion of Cdkn2a/2b in tremolite-induced malignant mesothelioma in rats
Cancer Sci
2020
111(4):1180-1192.
PubMed ID: 32080953
DOI: 10.1111/cas.14358
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16453
Ohara Y, Chew SH, Misawa N, Wang S, Somiya D, Nakamura K, Kajiyama H, Kikkawa F, Tsuyuki Y, Jiang L, Yamashita K, Sekido Y, Lipson KE, Toyokuni S.
Connective tissue growth factor-specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model
Oncotarget
2018
9(26):18494-18509
PubMed ID: 29719620
DOI: 10.18632/oncotarget.24892
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16454
Chew SH, Okazaki Y, Akatsuka S, Wang S, Jiang L, Ohara Y, Ito F, Saya H, Sekido Y, Toyokuni S.
Rheostatic CD44 isoform expression and its association with oxidative stress in human malignant mesothelioma
Free Radic Biol Med
2017
106:91-99
PubMed ID: 28185919
DOI: 10.1016/j.freeradbiomed.2017.02.011
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16459
Wang S, Jiang L, Han Y, Chew SH, Ohara Y, Akatsuka S, Weng L, Kawaguchi K, Fukui T, Sekido Y, Yokoi K, Toyokuni S.
Urokinase-type plasminogen activator receptor promotes proliferation and invasion with reduced cisplatin sensitivity in malignant mesothelioma
Oncotarget
2016
;7(43):69565-69578
PubMed ID: 27602956
DOI: 10.18632/oncotarget.11829
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16460
Li Q, Wang W, Machino Y, Yamada T, Kita K, Oshima M, Sekido Y, Tsuchiya M, Suzuki Y, Nan-Ya K, Iida S, Nakamura K, Iwakiri S, Itoi K, Yano S.
Therapeutic activity of glycoengineered anti-GM2 antibodies against malignant pleural mesothelioma
Cancer Sci
2015
106(1):102-7
PubMed ID: 25421609
DOI: 10.1111/cas.12575
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16455
Chew SH, Okazaki Y, Nagai H, Misawa N, Akatsuka S, Yamashita K, Jiang L, Yamashita Y, Noguchi M, Hosoda K, Sekido Y, Takahashi T, Toyokuni S.
Cancer-promoting role of adipocytes in asbestos-induced mesothelial carcinogenesis through dysregulated adipocytokine production
Carcinogenesis
2014
35(1):164-72
PubMed ID: 23917077
DOI: 10.1093/carcin/bgt267
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16461
Abe S, Morita Y, Kaneko MK, Hanibuchi M, Tsujimoto Y, Goto H, Kakiuchi S, Aono Y, Huang J, Sato S, Kishuku M, Taniguchi Y, Azuma M, Kawazoe K, Sekido Y, Yano S, Akiyama S, Sone S, Minakuchi K, Kato Y, Nishioka Y.
A novel targeting therapy of malignant mesothelioma using anti-podoplanin antibody
J Immunol
2013
190(12):6239-49
PubMed ID: 23690472
DOI: 10.4049/jimmunol.1300448
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16462
Satoh M, Takemura Y, Hamada H, Sekido Y, Kubota S.
EGCG induces human mesothelioma cell death by inducing reactive oxygen species and autophagy
Cancer Cell Int
2013
13(1):19
PubMed ID: 23432995
DOI: 10.1186/1475-2867-13-19
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16456
Horio M, Sato M, Takeyama Y, Elshazley M, Yamashita R, Hase T, Yoshida K, Usami N, Yokoi K, Sekido Y, Kondo M, Toyokuni S, Gazdar AF, Minna JD, Hasegawa Y.
Transient but not stable ZEB1 knockdown dramatically inhibits growth of malignant pleural mesothelioma cells
Ann Surg Oncol
2012
19 Suppl 3(Suppl 3):S634-45
PubMed ID: 22086445
DOI: 10.1245/s10434-011-2142-0
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16463
Takemura Y, Satoh M, Satoh K, Hamada H, Sekido Y, Kubota S.
High dose of ascorbic acid induces cell death in mesothelioma cells
Biochem Biophys Res Commun
2010
394(2):249-53
PubMed ID: 20171954
DOI: 10.1016/j.bbrc.2010.02.012
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16457
Suzuki Y, Murakami H, Kawaguchi K, Tanigushi T, Fujii M, Shinjo K, Kondo Y, Osada H, Shimokata K, Horio Y, Hasegawa Y, Hida T, Sekido Y.
Activation of the PI3K-AKT pathway in human malignant mesothelioma cells
Mol Med Rep
2009
2(2):181-8
PubMed ID: 21475810
DOI: 10.3892/mmr_00000081
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16464
Goto Y, Shinjo K, Kondo Y, Shen L, Toyota M, Suzuki H, Gao W, An B, Fujii M, Murakami H, Osada H, Taniguchi T, Usami N, Kondo M, Hasegawa Y, Shimokata K, Matsuo K, Hida T, Fujimoto N, Kishimoto T, Issa JP, Sekido Y.
Epigenetic profiles distinguish malignant pleural mesothelioma from lung adenocarcinoma
Cancer Res
2009
69(23):9073-82
PubMed ID: 19887624
DOI: 10.1158/0008-5472.CAN-09-1595
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16465
Yamada T, Yano S, Ogino H, Ikuta K, Kakiuchi S, Hanibuchi M, Kanematsu T, Taniguchi T, Sekido Y, Sone S.
Lysophosphatidic acid stimulates the proliferation and motility of malignant pleural mesothelioma cells through lysophosphatidic acid receptors, LPA1 and LPA2
Cancer Sci
2008
99(8):1603-10
PubMed ID: 18754873
DOI: 10.1111/j.1349-7006.2008.00848.x
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16458
Taniguchi T, Karnan S, Fukui T, Yokoyama T, Tagawa H, Yokoi K, Ueda Y, Mitsudomi T, Horio Y, Hida T, Yatabe Y, Seto M, Sekido Y.
Genomic profiling of malignant pleural mesothelioma with array-based comparative genomic hybridization shows frequent non-random chromosomal alteration regions including JUN amplification on 1p32
Cancer Sci
2007
98(3):438-46
PubMed ID: 17270034
DOI: 10.1111/j.1349-7006.2006.00386.x
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3337
Usami, Noriyasu, Fukui, Takayuki, Kondo, Masashi, Taniguchi, Tetsuo, Yokoyama, Toshihiko, Mori, Shoichi, Yokoi, Kohei, Horio, Yoshitsugu, Shimokata, Kaoru, Sekido, Yoshitaka, Hida, Toyoaki
Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients.
Cancer Sci
2006
97:387-94
PubMed ID: 16630136
DOI: 10.1111/j.1349-7006.2006.00184.x
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