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Cell No. : Cell Name
RCB5663 : SS-2  update : 2024/11/11
Comment
Comment from the depositorNeuroendocrine carcinoma cell line derived from a human ascending colon tumor.
Terms and conditions1) A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cancer Sci 2019 110(12):3708-3717) designated by the DEPOSITOR is required. 3) Co-authorship must be given to Seiichi Shinji when the results using the cells are published.
Remarks
approver's address
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English
Address
Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Nippon Medical School
1-1-5, Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan
Dr.Shinji Seiichi
Fax. +81-3-5685-0989
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SHINJI, Seiichi
Originator SHINJI, Seiichi
Year of deposit 2022
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 59 years
Tissue ascending colon
Disease name Colonic neuroendocrine carcinoma
Tumor Neuroendocrine carcinoma, T4aN1M1 Stage lV
Metastatic ability Yes
Metastatic tissue peritoneum
Classification cancer
Year of origin 2004
Lifespan infinite
Morphology epithelial-like
Contact inhibition No
Tumorigenesis Yes
Cellosaurus(Expasy) CVCL_YD51
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium RPMI‐1640 medium containing 10% FBS RPMI‐1640 + 10% FBS
Antibiotics Free Free
Passage method 0.25%Trypsin-EDTA 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Designated culture dish IWAKI Tissue Culture 60mm dish
Culture information Passage cell No 3-5x10 5 cells/60mm dish 5x10 5 cells/60mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Cell Banker 1 CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
16444  Shinji S, Sasaki N, Yamada T, Koizumi M, Ohta R, Matsuda A, Yokoyama Y, Takahashi G, Hotta M, Hara K, Takeda K, Ueda K, Kuriyama S, Ishiwata T, Ueda Y, Murakami T, Kanazawa Y, Yoshida H.  Establishment and characterization of a novel neuroendocrine carcinoma cell line derived from a human ascending colon tumor  Cancer Sci   2019  110(12):3708-3717  PubMed ID: 31648389   DOI: 10.1111/cas.14221

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