細胞番号 : 細胞名
RCB5662 : HPL1D
update : 2024/11/11
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細胞特性(Comment:英) | RCB5661 HPL1A、RCB5662 HPL1D were derived from the same patient. |
細胞特性(日) | RCB5661 HPL1A、RCB5662 HPL1Dは同一人由来。 |
細胞特性(寄託者記述:英) | Immortalized human peripheral lung epithelial cell line.The HPL1A and HPL1D lines were established by transfecting the SV40 large T antigen gene from the normal lung of a 74-year-old female donor, who had never smoked and underwent surgery for a small well-demarcated squamous cell carcinoma of the earliest disease stage (pT1N0M0, stage I). Precancerous regions of peripheral lung such as atypical adenomatous hyperplasia were not noted in the lung specimen by the conventional pathological examination. Considering the uniform integration pattern observed for the SV40 large T antigen gene, the HPL1A and HPL1D lines appear to have arisen from a single immortalized cell. |
細胞特性(寄託者記述:日) | ヒト正常末梢肺由来不死化気道上皮細胞株。 |
使用条件(英) | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
使用条件(日) | 研究成果の公表にあたって謝辞の表明を必要とする。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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寄託者 |
TAKAHASHI, Takashi
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樹立者 |
TAKAHASHI, Takashi
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寄託日 |
2022
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クローニング(寄託者) |
Yes
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クローニングの時期(寄託者) |
cloning ring method
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動物種 |
_human
< Mammals
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属名 |
Homo
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種名 |
sapiens
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性別 |
Female
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齢 |
74 years
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採取組織 |
normal peripheral lung specimens
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細胞分類 |
other
(immortalized)
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遺伝子改変 |
recombinant
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外来遺伝子 |
SV40 large T antigen gene
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細胞寿命 |
infinite
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細胞形態 |
epithelial-like
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Cellosaurus(Expasy) |
CVCL_8880
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
Adherent cells
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Adherent cells
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培地 |
F12 (Sigma-Aldrich #N6658) + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
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HamF12 + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
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抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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播種細胞数 |
3 x 10 5cells/ 10 cm dish
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3-6 x 10 5 cells/100 mm dis
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継代密度 |
1 : 5 split
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継代・培地交換頻度 |
every 3 days
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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培養最適温度 |
37
℃
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37
℃
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二酸化炭素濃度 |
5
%
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5
%
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凍結培地 |
CELLBANKER (ZENOGEN PHARMA #DS-CB011)
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CELLBANKER 1
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凍結方法 |
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Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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動物種PCR |
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OK
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個体識別検査 |
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OK
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文献情報 |
Reference(英) |
17件
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Reference(日) |
0件
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利用者成果(英) |
0件
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利用者成果(日) |
0件
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Reference(英) |
16269
Deng H, Liu H, de Silva T, Xue Y, Mohamud Y, Ng CS, Qu J, Zhang J, Jia WWG, Lockwood WW, Luo H.
Coxsackievirus Type B3 Is a Potent Oncolytic Virus against KRAS-Mutant Lung Adenocarcinoma
Mol Ther Oncolytics
2019
14:266-278
PubMed ID: 31463367
DOI: 10.1016/j.omto.2019.07.003
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16268
Person RJ, Ngalame NN, Makia NL, Bell MW, Waalkes MP, Tokar EJ.
Chronic inorganic arsenic exposure in vitro induces a cancer cell phenotype in human peripheral lung epithelial cells
Toxicol Appl Pharmacol
2015
286(1):36-43
PubMed ID: 25804888
DOI: 10.1016/j.taap.2015.03.014
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16267
Jala VR, Radde BN, Haribabu B, Klinge CM.
Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
BMC Cancer
2012
12:624
PubMed ID: 23273253
DOI: 10.1186/1471-2407-12-624
|
16266
Al-Wadei HA, Al-Wadei MH, Masi T, Schuller HM.
Chronic exposure to estrogen and the tobacco carcinogen NNK cooperatively modulates nicotinic receptors in small airway epithelial cells
Lung Cancer
2010
69(1):33-9.
PubMed ID: 19896235
DOI: 10.1016/j.lungcan.2009.09.011
|
16265
Schuller HM, Al-Wadei HA, Majidi M.
Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma
Carcinogenesis
2008
29(10):1979-85
PubMed ID: 18310090
DOI: 10.1093/carcin/bgn041
|
16261
Romanowska M, Kikawa KD, Fields JR, Maciag A, North SL, Shiao YH, Kasprzak KS, Anderson LM.
Effects of selenium supplementation on expression of glutathione peroxidase isoforms in cultured human lung adenocarcinoma cell lines
Lung Cancer
2007
55(1):35-42
PubMed ID: 17052796
DOI: 10.1016/j.lungcan.2006.09.007
|
16263
Ranganathan P, Agrawal A, Bhushan R, Chavalmane AK, Kalathur RK, Takahashi T, Kondaiah P.
Expression profiling of genes regulated by TGF-beta: differential regulation in normal and tumour cells
BMC Genomics
2007
8:98
PubMed ID: 17425807
DOI: 10.1186/1471-2164-8-98
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16264
Romanowska M, Maciag A, Smith AL, Fields JR, Fornwald LW, Kikawa KD, Kasprzak KS, Anderson LM.
DNA damage, superoxide, and mutant K-ras in human lung adenocarcinoma cells
Free Radic Biol Med
2007
43(8):1145-55
PubMed ID: 17854710
DOI: 10.1016/j.freeradbiomed.2007.07.004
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16245
Sato H, Yazawa T, Suzuki T, Shimoyamada H, Okudela K, Ikeda M, Hamada K, Yamada-Okabe H, Yao M, Kubota Y, Takahashi T, Kamma H, Kitamura H.
Growth regulation via insulin-like growth factor binding protein-4 and -2 in association with mutant K-ras in lung epithelia
Am J Pathol
2006
169(5):1550-66
PubMed ID: 17071580
DOI: 10.2353/ajpath.2006.051068
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16262
Al-Wadei HA, Schuller HM.
Cyclic adenosine monophosphate-dependent cell type-specific modulation of mitogenic signaling by retinoids in normal and neoplastic lung cells
Cancer Detect Prev
2006
30(5):403-11
PubMed ID: 17067750
DOI: 10.1016/j.cdp.2006.07.008
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16260
Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido Y, Takahashi T.
A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation
Cancer Res
2005
65(21):9628-32.
PubMed ID: 16266980
DOI: 10.1158/0008-5472.CAN-05-2352
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16258
Okudela K, Hayashi H, Ito T, Yazawa T, Suzuki T, Nakane Y, Sato H, Ishi H, KeQin X, Masuda A, Takahashi T, Kitamura H.
K-ras gene mutation enhances motility of immortalized airway cells and lung adenocarcinoma cells via Akt activation: possible contribution to non-invasive expansion of lung adenocarcinoma
Am J Pathol
2004
164(1):91-100
PubMed ID: 14695323
DOI: 10.1016/S0002-9440(10)63100-8
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16259
Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, Harano T, Yatabe Y, Nagino M, Nimura Y, Mitsudomi T, Takahashi T.
Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival
Cancer Res
2004
64(11):3753-6
PubMed ID: 15172979
DOI: 10.1158/0008-5472.CAN-04-0637
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16256
Sithanandam G, Smith GT, Masuda A, Takahashi T, Anderson LM, Fornwald LW.
Cell cycle activation in lung adenocarcinoma cells by the ErbB3/phosphatidylinositol 3-kinase/Akt pathway
Carcinogenesis
2003
24(10):1581-92
PubMed ID: 12896906
DOI: 10.1093/carcin/bgg125
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16257
Cheng RY, Zhao A, Alvord WG, Powell DA, Bare RM, Masuda A, Takahashi T, Anderson LM, Kasprzak KS.
Gene expression dose-response changes in microarrays after exposure of human peripheral lung epithelial cells to nickel(II)
Toxicol Appl Pharmacol
2003
191(1):22-39
PubMed ID: 12915101
DOI: 10.1016/s0041-008x(03)00228-x
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16255
Kammouni W, Ramakrishna G, Sithanandam G, Smith GT, Fornwald LW, Masuda A, Takahashi T, Anderson LM.
Increased K-ras protein and activity in mouse and human lung epithelial cells at confluence
Cell Growth Differ
2002
13(9):441-8
PubMed ID: 12354753
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16243
Masuda A, Kondo M, Saito T, Yatabe Y, Kobayashi T, Okamoto M, Suyama M, Takahashi T, Takahashi T.
Establishment of human peripheral lung epithelial cell lines (HPL1) retaining differentiated characteristics and responsiveness to epidermal growth factor, hepatocyte growth factor, and transforming growth factor beta1
Cancer Res
1997
57(21):4898-904
PubMed ID: 9354455
|