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Cell No. : Cell Name
RCB5662 : HPL1D  update : 2024/11/11
CommentRCB5661 HPL1A、RCB5662 HPL1D were derived from the same patient.
Comment from the depositorImmortalized human peripheral lung epithelial cell line.The HPL1A and HPL1D lines were established by transfecting the SV40 large T antigen gene from the normal lung of a 74-year-old female donor, who had never smoked and underwent surgery for a small well-demarcated squamous cell carcinoma of the earliest disease stage (pT1N0M0, stage I). Precancerous regions of peripheral lung such as atypical adenomatous hyperplasia were not noted in the lung specimen by the conventional pathological examination. Considering the uniform integration pattern observed for the SV40 large T antigen gene, the HPL1A and HPL1D lines appear to have arisen from a single immortalized cell.　　
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor TAKAHASHI, Takashi
Originator TAKAHASHI, Takashi
Year of deposit 2022
Cloning (depositor) Yes
_Date (depositor) cloning ring method
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 74 years
Tissue normal peripheral lung specimens
Classification other (immortalized)
Recombinant recombinant
Exogene SV40 large T antigen gene
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8880
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium F12 (Sigma-Aldrich #N6658) + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine HamF12 + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10 5cells/ 10 cm dish 3-6 x 10 5 cells/100 mm dis
Passage ratio 1 : 5 split
SC frequency every 3 days Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER (ZENOGEN PHARMA #DS-CB011) CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 17
User's Publication 0


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Reference
16269  Deng H, Liu H, de Silva T, Xue Y, Mohamud Y, Ng CS, Qu J, Zhang J, Jia WWG, Lockwood WW, Luo H.  Coxsackievirus Type B3 Is a Potent Oncolytic Virus against KRAS-Mutant Lung Adenocarcinoma  Mol Ther Oncolytics  2019  14:266-278  PubMed ID: 31463367   DOI: 10.1016/j.omto.2019.07.003
16268  Person RJ, Ngalame NN, Makia NL, Bell MW, Waalkes MP, Tokar EJ.  Chronic inorganic arsenic exposure in vitro induces a cancer cell phenotype in human peripheral lung epithelial cells  Toxicol Appl Pharmacol  2015  286(1):36-43  PubMed ID: 25804888   DOI: 10.1016/j.taap.2015.03.014
16267  Jala VR, Radde BN, Haribabu B, Klinge CM.  Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer  BMC Cancer  2012  12:624  PubMed ID: 23273253   DOI: 10.1186/1471-2407-12-624
16266  Al-Wadei HA, Al-Wadei MH, Masi T, Schuller HM.  Chronic exposure to estrogen and the tobacco carcinogen NNK cooperatively modulates nicotinic receptors in small airway epithelial cells  Lung Cancer  2010  69(1):33-9.  PubMed ID: 19896235   DOI: 10.1016/j.lungcan.2009.09.011
16265  Schuller HM, Al-Wadei HA, Majidi M.  Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma  Carcinogenesis  2008  29(10):1979-85  PubMed ID: 18310090   DOI: 10.1093/carcin/bgn041
16261  Romanowska M, Kikawa KD, Fields JR, Maciag A, North SL, Shiao YH, Kasprzak KS, Anderson LM.  Effects of selenium supplementation on expression of glutathione peroxidase isoforms in cultured human lung adenocarcinoma cell lines  Lung Cancer  2007  55(1):35-42  PubMed ID: 17052796   DOI: 10.1016/j.lungcan.2006.09.007
16263  Ranganathan P, Agrawal A, Bhushan R, Chavalmane AK, Kalathur RK, Takahashi T, Kondaiah P.  Expression profiling of genes regulated by TGF-beta: differential regulation in normal and tumour cells  BMC Genomics  2007  8:98  PubMed ID: 17425807   DOI: 10.1186/1471-2164-8-98
16264  Romanowska M, Maciag A, Smith AL, Fields JR, Fornwald LW, Kikawa KD, Kasprzak KS, Anderson LM.  DNA damage, superoxide, and mutant K-ras in human lung adenocarcinoma cells  Free Radic Biol Med  2007  43(8):1145-55  PubMed ID: 17854710   DOI: 10.1016/j.freeradbiomed.2007.07.004
16245  Sato H, Yazawa T, Suzuki T, Shimoyamada H, Okudela K, Ikeda M, Hamada K, Yamada-Okabe H, Yao M, Kubota Y, Takahashi T, Kamma H, Kitamura H.  Growth regulation via insulin-like growth factor binding protein-4 and -2 in association with mutant K-ras in lung epithelia  Am J Pathol   2006  169(5):1550-66  PubMed ID: 17071580   DOI: 10.2353/ajpath.2006.051068
16262  Al-Wadei HA, Schuller HM.  Cyclic adenosine monophosphate-dependent cell type-specific modulation of mitogenic signaling by retinoids in normal and neoplastic lung cells  Cancer Detect Prev  2006  30(5):403-11  PubMed ID: 17067750   DOI: 10.1016/j.cdp.2006.07.008
16260  Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido Y, Takahashi T.  A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation  Cancer Res  2005  65(21):9628-32.  PubMed ID: 16266980   DOI: 10.1158/0008-5472.CAN-05-2352
16258  Okudela K, Hayashi H, Ito T, Yazawa T, Suzuki T, Nakane Y, Sato H, Ishi H, KeQin X, Masuda A, Takahashi T, Kitamura H.  K-ras gene mutation enhances motility of immortalized airway cells and lung adenocarcinoma cells via Akt activation: possible contribution to non-invasive expansion of lung adenocarcinoma  Am J Pathol  2004  164(1):91-100  PubMed ID: 14695323   DOI: 10.1016/S0002-9440(10)63100-8
16259  Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H, Harano T, Yatabe Y, Nagino M, Nimura Y, Mitsudomi T, Takahashi T.  Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival  Cancer Res  2004  64(11):3753-6  PubMed ID: 15172979   DOI: 10.1158/0008-5472.CAN-04-0637
16256  Sithanandam G, Smith GT, Masuda A, Takahashi T, Anderson LM, Fornwald LW.  Cell cycle activation in lung adenocarcinoma cells by the ErbB3/phosphatidylinositol 3-kinase/Akt pathway  Carcinogenesis  2003  24(10):1581-92  PubMed ID: 12896906   DOI: 10.1093/carcin/bgg125
16257  Cheng RY, Zhao A, Alvord WG, Powell DA, Bare RM, Masuda A, Takahashi T, Anderson LM, Kasprzak KS.  Gene expression dose-response changes in microarrays after exposure of human peripheral lung epithelial cells to nickel(II)  Toxicol Appl Pharmacol  2003  191(1):22-39  PubMed ID: 12915101   DOI: 10.1016/s0041-008x(03)00228-x
16255  Kammouni W, Ramakrishna G, Sithanandam G, Smith GT, Fornwald LW, Masuda A, Takahashi T, Anderson LM.  Increased K-ras protein and activity in mouse and human lung epithelial cells at confluence  Cell Growth Differ  2002  13(9):441-8  PubMed ID: 12354753  
16243  Masuda A, Kondo M, Saito T, Yatabe Y, Kobayashi T, Okamoto M, Suyama M, Takahashi T, Takahashi T.  Establishment of human peripheral lung epithelial cell lines (HPL1) retaining differentiated characteristics and responsiveness to epidermal growth factor, hepatocyte growth factor, and transforming growth factor beta1  Cancer Res  1997  57(21):4898-904  PubMed ID: 9354455  

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