Cell No. : Cell Name
RCB5661 : HPL1A
update : 2024/02/07
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| Comment | RCB5661 HPL1A、RCB5662 HPL1D were derived from the same patient. |
| Comment from the depositor | Immortalized human peripheral lung epithelial cell line.The HPL1A and HPL1D lines were established by transfecting the SV40 large T antigen gene from the normal lung of a 74-year-old female donor, who had never smoked and underwent surgery for a small well-demarcated squamous cell carcinoma of the earliest disease stage (pT1N0M0, stage I). Precancerous regions of peripheral lung such as atypical adenomatous hyperplasia were not noted in the lung specimen by the conventional pathological examination. Considering the uniform integration pattern observed for the SV40 large T antigen gene, the HPL1A and HPL1D lines appear to have arisen from a single immortalized cell. |
| Terms and conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
TAKAHASHI, Takashi
|
| Originator |
TAKAHASHI, Takashi
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| Year of deposit |
2022
|
| Cloning (depositor) |
Yes
|
| _Method (depositor) |
cloning ring method
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| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Female
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| Age at sampling |
74 years
|
| Primary focus |
normal peripheral lung specimens
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| Classification |
other
(immortalized)
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| Recombinant |
recombinant
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| Exogene |
SV40 large T antigen gene
|
|
Lifespan |
infinite
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| Morphology |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_9863
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Adherent cells
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Adherent cells
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| Culture medium |
F12 (Sigma-Aldrich #N6658) + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
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HamF12 + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage cell No |
3 x 10 5cells/ 10 cm dish
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3-6 x 10 5 cells/100 mm dish
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| Passage ratio |
1 : 5 split
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| SC frequency |
every 3 days
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER (ZENOGEN PHARMA #DS-CB011)
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CELLBANKER 1
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| Freezing method |
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Slow freezing
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| Mycoplasma |
|
(-)
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| Animal PCR |
|
OK
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| STR(human) |
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OK
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| Reference information |
Reference |
12
|
| User's Publication |
0
|
| Reference |
|
16254
Vu T, Yang S, Datta PK.
MiR-216b/Smad3/BCL-2 Axis Is Involved in Smoking-Mediated Drug Resistance in Non-Small Cell Lung Cancer
Cancers (Basel)
2020
12(7):1879
PubMed ID: 32668597
DOI: 10.3390/cancers12071879
|
|
16253
Almosnid NM, Gao Y, He C, Park HS, Altman E.
In vitro antitumor effects of two novel oligostilbenes, cis- and trans-suffruticosol D, isolated from Paeonia suffruticosa seeds
Int J Oncol
2016
48(2):646-56.
PubMed ID: 26647827
DOI: 10.3892/ijo.2015.3269
|
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16252
Gao Y, He C, Ran R, Zhang D, Li D, Xiao PG, Altman E.
The resveratrol oligomers, cis- and trans-gnetin H, from Paeonia suffruticosa seeds inhibit the growth of several human cancer cell lines
J Ethnopharmacol
2015
169:24-33
PubMed ID: 25862967
DOI: 10.1016/j.jep.2015.03.074
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16251
Roth FC, Mulder JE, Brien JF, Takahashi T, Massey TE.
Cytotoxic interaction between amiodarone and desethylamiodarone in human peripheral lung epithelial cells
Chem Biol Interact
2013
204(3):135-9
PubMed ID: 23707191
DOI: 10.1016/j.cbi.2013.05.006
|
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16249
Samanta D, Gonzalez AL, Nagathihalli N, Ye F, Carbone DP, Datta PK.
Smoking attenuates transforming growth factor-β-mediated tumor suppression function through downregulation of Smad3 in lung cancer
Cancer Prev Res (Phila)
2012
5(3):453-63
PubMed ID: 22232600
DOI: 10.1158/1940-6207.CAPR-11-0313
|
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16250
Samanta D, Kaufman J, Carbone DP, Datta PK.
Long-term smoking mediated down-regulation of Smad3 induces resistance to carboplatin in non-small cell lung cancer
Neoplasia
2012
14(7):644-55
PubMed ID: 22904681
DOI: 10.1593/neo.12548
|
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16248
Mulder JE, Brien JF, Racz WJ, Takahashi T, Massey TE.
Mechanisms of amiodarone and desethylamiodarone cytotoxicity in nontransformed human peripheral lung epithelial cells
J Pharmacol Exp Ther
2011
336(2):551-9
PubMed ID: 21078785
DOI: 10.1124/jpet.110.173120
|
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16247
Nicolescu AC, Ji Y, Comeau JL, Hill BC, Takahashi T, Brien JF, Racz WJ, Massey TE.
Direct mitochondrial dysfunction precedes reactive oxygen species production in amiodarone-induced toxicity in human peripheral lung epithelial HPL1A cells
Toxicol Appl Pharmacol
2008
227(3):370-9
PubMed ID: 18191165
DOI: 10.1016/j.taap.2007.12.009
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16246
Nicolescu AC, Comeau JL, Hill BC, Bedard LL, Takahashi T, Brien JF, Racz WJ, Massey TE
Aryl radical involvement in amiodarone-induced pulmonary toxicity: investigation of protection by spin-trapping nitrones
Toxicol Appl Pharmacol
2007
220(1):60-71
PubMed ID: 17316728
DOI: 10.1016/j.taap.2006.12.031
|
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16245
Sato H, Yazawa T, Suzuki T, Shimoyamada H, Okudela K, Ikeda M, Hamada K, Yamada-Okabe H, Yao M, Kubota Y, Takahashi T, Kamma H, Kitamura H.
Growth regulation via insulin-like growth factor binding protein-4 and -2 in association with mutant K-ras in lung epithelia
Am J Pathol
2006
169(5):1550-66
PubMed ID: 17071580
DOI: 10.2353/ajpath.2006.051068
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16244
Martinez JM, Afshari CA, Bushel PR, Masuda A, Takahashi T, Walker NJ.
Differential toxicogenomic responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin in malignant and nonmalignant human airway epithelial cells
Toxicol Sci
2002
69(2):409-23
PubMed ID: 12377990
DOI: 10.1093/toxsci/69.2.409
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16243
Masuda A, Kondo M, Saito T, Yatabe Y, Kobayashi T, Okamoto M, Suyama M, Takahashi T, Takahashi T.
Establishment of human peripheral lung epithelial cell lines (HPL1) retaining differentiated characteristics and responsiveness to epidermal growth factor, hepatocyte growth factor, and transforming growth factor beta1
Cancer Res
1997
57(21):4898-904
PubMed ID: 9354455
|
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