Cell No. : Cell Name RCB5658 : HeLa PMP2 KO  update : 2024/01/17
Comment
Comment from the depositor			PMP2-knockout HeLa cells were generated with CRISPR/Cas9 gene editing.
Terms and conditions			1) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Rep 2021 37(6):109935) designated by the DEPOSITOR is required.
Remarks			CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form			Order Form(C-0005.pdf)   MTA(C-0065.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	SAKO, Yasushi
		Originator	SAKO, Yasushi
		Year of deposit	2022
		Original cell	HeLa
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	31 years
		Tissue	cervix
		Primary focus	cervical carcinoma
		Classification	cancer
		Recombinant	recombinant
		Lifespan	infinite
		Morphology	epithelial-like
			deposit info	lot info
		Medium	Medium List
		Culture type	Adherent cells	Adherent cells
		Culture medium	DMEM + 10% FBS	DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 10-20 split
		SC frequency		Subculture : 2 times/week
		Temperature	37 ℃	37 ℃
		CO2 concentration	5 %	5 %
		Freeze medium	Medium + 10% DMSO	Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Reference information		Reference	1
		User's Publication	0

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Reference

16242  Abe M, Makino A, Murate M, Hullin-Matsuda F, Yanagawa M, Sako Y, Kobayashi T.  PMP2/FABP8 induces PI(4,5)P 2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane  Cell Rep  2021  37(6):109935  PubMed ID: 34758297   DOI: 10.1016/j.celrep.2021.109935

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