Cell No. : Cell Name
RCB5657 : 2G11
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Mesenchymal progenitor cells derived from rat skeletal muscle, with the ability to differentiate into adipocytes and fibroblasts. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Anim Sci J 2020 91(1):e13368) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
IPR |
The University of Tokyo (Japan)
|
| Depositor |
YAMANOUCHI, Keitaro
|
| Originator |
YAMANOUCHI, Keitaro
|
| Year of deposit |
2022
|
| Cloning (depositor) |
Yes
|
| _Date (depositor) |
2005
|
| Animal |
_rat
< Mammals
|
| Strain name |
Wistar-Imamichi
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| Genus |
Rattus
|
| Species |
norvegicus
|
| Gender |
Male
|
| Age at sampling |
6 weeks
|
| Tissue |
soleus muscle
|
| Classification |
normal
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Differentiation |
Pretreatment with bFGF(10ng/mL) is required to gain adipogenicity.
|
| Surface antigen (depositor) |
CSPG4
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM(GIBCO#11995-073)+10%FBS
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
Trypsin, 80% confluent
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0.25% Trypsin
|
| Designated culture dish |
IWAKI poly-L-Lysine Coatplate
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IWAKI poly-L-Lysine Coatplate : 60mm dish(#4010-040), 100mm dish(#4020-040)
|
| Coating dish |
50-100 μg/mLfibronectin(GIBCO#33016015)
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50 μg/mL fibronectin
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Culture information
|
Passage ratio |
|
1 : 10-20 split
|
| SC frequency |
|
Subculture : 2-3 times/week (not confluent)
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER 2(Takara #CB031)
|
CELLBANKER 2
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
16025
Takeuchi S, Yamanouchi K, Sugihara H, Matsuwaki T, Nishihara M.
Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
Anim Sci J
2020
91(1):e13368
PubMed ID: 32285501
DOI: 10.1111/asj.13368
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16024
Sugihara H, Teramoto N, Yamanouchi K, Matsuwaki T, Nishihara M.
Oxidative stress-mediated senescence in mesenchymal progenitor cells causes the loss of their fibro/adipogenic potential and abrogates myoblast fusion
Aging (Albany NY)
2018
10(4):747-763
PubMed ID: 29695641
DOI: 10.18632/aging.101425
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16023
Nakano S, Nakamura K, Teramoto N, Yamanouchi K, Nishihara M.
Basic fibroblast growth factor is pro-adipogenic in rat skeletal muscle progenitor clone, 2G11 cells
Anim Sci J
2016
87(1):99-108
PubMed ID: 26154243
DOI: 10.1111/asj.12397
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16022
Takeuchi S, Nakano S, Nakamura K, Ozoe A, Chien P, Yoshihara H, Hakuno F, Matsuwaki T, Saeki Y, Takahashi S, Yamanouchi K, Nishihara M.
Roles of chondroitin sulfate proteoglycan 4 in fibrogenic/adipogenic differentiation in skeletal muscle tissues
Exp Cell Res
2016
347(2):367-77
PubMed ID: 27582000
DOI: 10.1016/j.yexcr.2016.08.023
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16021
Murakami Y, Yada E, Nakano S, Miyagoe-Suzuki Y, Hosoyama T, Matsuwaki T, Yamanouchi K, Nishihara M.
Establishment of bipotent progenitor cell clone from rat skeletal muscle
Anim Sci J
2011
82(6):764-72
PubMed ID: 22111633
DOI: 10.1111/j.1740-0929.2011.00907.x
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