Cell No. : Cell Name
RCB5503 : RAW-D cell
update : 2024/01/25
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| Comment | |
| Comment from the depositor | Sub-clone of RAW-264 cells: Osteoclast precursor cell line efficiently differentiates into multinucleated osteoclasts by the stimulation with RANKL. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (J Endocrinol 2004 180(1):193-201, J Exp Med 2004 200(7):941-6) designated by the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KUKITA, Toshio
|
| Originator |
KUKITA, Toshio
|
| Year of deposit |
2021
|
| Another name |
RAW264 cell D-clone
|
| Original cell |
RAW264
|
| Animal |
_mouse
< Mammals
|
| Genus |
mus
|
| Species |
musculus
|
| Gender |
Unknown
|
| Tissue |
leukemic monocyte
|
| Classification |
transformed
|
|
Lifespan |
infinite
|
| Morphology |
other
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
αMEM + 10％FBS(heat inactivated)
|
MEM ALPHA + 10% FBS (heat inactivated)
|
| Antibiotics |
Streptomycin, Penicillin
|
Free
|
|
Passage method |
|
pipetting
|
Culture information
|
Passage cell No |
2.5-5x10 5 cells/60mm dish
|
2.5-5x10 5 cells/60mm dish
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Others |
Remove old medium and add fresh medium. Detach cells by a repeated mild pipetting by use of the sterile Pasteur pipette with marrow tips but with soft-ended hole prepared by burning the tip of the pipette. Refrain from excess number of passages, or differentiation efficiency of osteoclasts will be decreased. Refrain from collecting tightly adherent cells during pipetting. Aliquots of cells should be frozen in the early passages of RAW-D cells if sufficient formation of osteoclasts were confirmed in the parallel differentiation culture. Preparation of many stocks of the frozen cells is highly recommended in order to utilize the same quality of RAW-D cells at any time researchers want to perform differentiation experiments. If excess numbers of cells are seeded in the differentiation experiments, osteoclast formation will markedly be suppressed. Researchers should seed the exact number of cells written in the above protocol in the differentiation experiments.
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| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
13691
Kukita A, Ichigi Y, Takigawa I, Watanabe T, Kukita T, Miyamoto H.
Infection of RANKL-primed RAW-D macrophages with Porphyromonas gingivalis promotes osteoclastogenesis in a TNF-α-independent manner
PLoS One
2012
7(6):e38500
PubMed ID: 22723864
DOI: 10.1371/journal.pone.0038500
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16408
Watanabe T, Kukita T, Kukita A, Wada N, Toh K, Nagata K, Nomiyama H, Iijima T.
Direct stimulation of osteoclastogenesis by MIP-1alpha: evidence obtained from studies using RAW264 cell clone highly responsive to RANKL
J Endocrinol
2004
180(1):193-201
PubMed ID: 14709158
DOI: 10.1677/joe.0.1800193
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|
16409
Kukita T, Wada N, Kukita A, Kakimoto T, Sandra F, Toh K, Nagata K, Iijima T, Horiuchi M, Matsusaki H, Hieshima K, Yoshie O, Nomiyama H.
RANKL-induced DC-STAMP is essential for osteoclastogenesis
J Exp Med
2004
200(7):941-6
PubMed ID: 15452179
DOI: 10.1084/jem.20040518
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