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Cell No. : Cell Name
RCB5481 : hFGFR3c/BaF3 update : 2026/03/27 |
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| Comment | ||||
| Comment from the depositor | The FGF receptor-overexpressing cells to be used for the analyses of receptor specificity of the various FGF ligands. These cells are engineered from Ba/F3 cells and stably overexpress one of seven subtypes, as defined in the cell name, of FGF receptors. When a cognate (specific) FGF ligand is added, together with heparin, to the medium of these cells, cell growth is stimulated that can be detected by cell number and/or DNA synthesis. While the parent Ba/F3 cells proliferate only by the stimulation with IL-3. | |||
| Terms and conditions | 1) A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (J Biol Chem 2011 286(30):26418-23, J Biomol Screen 2013 18(3):321-30) designated by the DEPOSITOR is requested. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | IMAMURA, Toru | ||
| Originator | IMAMURA, Toru | |||
| Year of deposit | 2021 | |||
| Original cell | Ba/F3 | |||
| Animal | _mouse < Mammals | |||
| Strain name | C3H | |||
| Genus | Mus | |||
| Species | musculus | |||
| Tissue | bone marrow | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | FGF receotor | |||
| 8.9k base | ||||
| Vector | MIRB | |||
| Selection | G418 1mg/ml | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| SDS |
SDS_DMSO(Japanese) 
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| Medium | Medium List | Culture type | Suspension cells | |
| Culture method |
General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium | RPMI1640 + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium) + 1 mg/ml G418 | Passage method | dilution | Culture information | Passage cell No | 1 x 10 5 cells/ml |
| Passage ratio | 1:20 | |||
| SC frequency | Subculture : every 2 days | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | RPMI1640 + 20% FBS + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Animal PCR | OK | |||
| SSLP(mouse) | OK | |||
| Saturation density | less than 2 x 10 6 cells/ml | |||
| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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