Cell No. : Cell Name
RCB5478 : hFGFR2b/BaF3
update : 2024/01/17
|
| Comment | |
| Comment from the depositor | The FGF receptor-overexpressing cells to be used for the analyses of receptor specificity of the various FGF ligands. These cells are engineered from Ba/F3 cells and stably overexpress one of seven subtypes, as defined in the cell name, of FGF receptors. When a cognate (specific) FGF ligand is added, together with heparin, to the medium of these cells, cell growth is stimulated that can be detected by cell number and/or DNA synthesis. While the parent Ba/F3 cells proliferate only by the stimulation with IL-3. |
| Terms and conditions | A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
IMAMURA, Toru
|
| Originator |
IMAMURA, Toru
|
| Year of deposit |
2021
|
| Original cell |
Ba/F3
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Tissue |
bone marrow
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
FGF receotor
|
|
8.9
base
|
| Vector |
MIRB
|
| Selection |
G418 1mg/ml
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture medium |
RPMI1640 + 10%FBS + 10% WEHI-3 conditioned medium + 1 mg/ml G418
|
RPMI1640 + 10% FBS + 10% (RCB0035 WEHI-3 conditioned medium) + 1 mg/ml G418
|
| Culture method |
|
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
1 x 10 5 cells/ml
|
1 x 10 5 cells/ml
|
| Passage ratio |
1:20
|
1:20
|
| SC frequency |
Subculture : every 2 days
|
Subculture : every 2 days
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
20%FBS+10%DMSO / RPMI1640
|
RPMI1640 + 20% FBS + 10% DMSO
|
| Freezing method |
Slow freezing
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Saturation density |
Cell density should not exceed 2 x 10 6 cells/ml, as otherwise, the cells tend to show decreased sensitivity to FGF stimuli and decreased growth rate.
|
less than 2 x 10 6 cells/ml
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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