Cell No. : Cell Name
RCB5394 : Y-ML-1B
update : 2024/01/17
|
| Comment | |
| Comment from the depositor | Large-cell lung cancer cell line producing granulocyte colony-stimulating factor. |
| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Cancer Genet Cytogenet 2002 137(1):33-42) designated by the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
SEKIDO, Yoshitaka
|
| Originator |
SEKIDO, Yoshitaka
|
| Year of deposit |
2021
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
70 years
|
| Tissue |
lung
|
| Primary focus |
lung
|
| Disease name |
large cell lung cancer
|
| Metastatic ability |
Unknown
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
| Year of origin |
2000
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Product |
G-CSF
|
| Cellosaurus(Expasy) |
CVCL_4779
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
RPMI-1640（Sigma）+10% Fetal bovine serum
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 1-2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER：Juji Field
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Doubling time |
19
hr
|
|
| STR(human) |
|
OK
|
|
deposit info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
9,13
|
8
|
11
|
9
|
17
|
9
|
X,Y
|
8,11
|
11,12
|
|
|
lot info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
9,13
|
8
|
11
|
9
|
17
|
9
|
X,Y
|
8,11
|
11,12
|
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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