Cell No. : Cell Name
RCB5389 : Rab1-CKO MDCK-II
update : 2024/07/09
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| Comment | |
| Comment from the depositor | Rab1 conditional KO MDCK-II cell line.
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| Terms and conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (J Cell Sci 2021 134(24):jcs259184 ) designated by the DEPOSITOR is requested. |
| Remarks | The users of this cell line must refer to Announcement of bioresources containing the tetracycline-inducible gene expression (TET) system. |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
FUKUDA, Mitsunori
|
| Originator |
FUKUDA, Mitsunori
|
| Year of deposit |
2021
|
| Original cell |
MDCK-II (RCB5148)
|
| Animal |
_dog
< Mammals
|
| Genus |
Canis
|
| Species |
lupus familiaris
|
| Gender |
Female
|
| Tissue |
kidney
|
| Classification |
transformed
|
| Exogene |
TetOn-Myc-OsTIR1 (puro), FLAG-AID-Rab1A (bsr)
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| Vector |
pMRX
|
| Selection |
puromycin (2μg/mL), blasticidin S(10μg/mL)
|
| Recombinant virus |
No
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM + 10% FBS
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DMEM (high glucose, without Sodium pyruvate) + 10% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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Culture information
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Passage cell No |
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1x10 5 cells / 60mm dish
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| SC frequency |
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Subculture : 2-3 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER 1plus
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CELLBANKER 1
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| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Reference information |
Reference |
1
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| User's Publication |
0
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