Cell No. : Cell Name
RCB5388 : HeLaM
update : 2024/11/11
|
| Comment | |
| Comment from the depositor | Human cervical cancer cell line HeLaM (substrain of HeLa). |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
FUKUDA, Mitsunori
|
| Year of deposit |
2021
|
| Original cell |
HeLa
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_R965
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM + 10% FBS
|
DMEM (high glucose, without Sodium pyruvate) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
(6-8x10 5 cells / 100mm dish) or (1 : 10-20)
|
| SC frequency |
|
Subculture : 2-3 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10%DMSO
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
22498
Rinka Hata, Akira Sugawara, Mitsunori Fukuda
The conserved K3 residue in the N-terminal region of Rab10 small GTPase is required for tubular endosome formation: N-terminal tagging causes Rab10 dysfunction
J Cell Sci
2025
PubMed ID: 39783278
DOI: 10.1242/jcs.263649
|
|
17743
Nakashima S, Matsui T, Fukuda M.
Vps9d1 regulates tubular endosome formation through specific activation of Rab22A
J Cell Sci
2023
136(6):jcs260522
PubMed ID: 36762583
DOI: 10.1242/jcs.260522
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|
22499
Komori T, Kuwahara T, Fujimoto T, Sakurai M, Koyama-Honda I, Fukuda M, Iwatsubo T.
Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2.
J Cell Sci
2023
136(14)
PubMed ID: 37365944
DOI: 10.1242/jcs.261003
|
|
17692
Hiragi S, Matsui T, Sakamaki Y, Fukuda M.
TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1
J Cell Biol
2022
221(12):e202201114
PubMed ID: 36197338
DOI: 10.1083/jcb.202201114
|
|
6047
Etoh K, Fukuda M.
Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors.
J Cell Sci
2019
132(5). pii: jcs226977
PubMed ID: 30700496
DOI: 10.1242/jcs.226977
|
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