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Cell No. : Cell Name
RCB5388 : HeLaM  update : 2024/07/09
Comment
Comment from the depositorHuman cervical cancer cell line HeLaM (substrain of HeLa).
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor FUKUDA, Mitsunori
Year of deposit 2021
Original cell HeLa
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant non-recombinant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_R965
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium DMEM + 10% FBS DMEM (high glucose, without Sodium pyruvate) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No (6-8x10 5 cells / 100mm dish) or (1 : 10-20)
SC frequency Subculture : 2-3 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10%DMSO CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
17743  Nakashima S, Matsui T, Fukuda M.  Vps9d1 regulates tubular endosome formation through specific activation of Rab22A  J Cell Sci  2023  136(6):jcs260522  PubMed ID: 36762583   DOI: 10.1242/jcs.260522
17692  Hiragi S, Matsui T, Sakamaki Y, Fukuda M.  TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1  J Cell Biol  2022  221(12):e202201114  PubMed ID: 36197338   DOI: 10.1083/jcb.202201114
6047  Etoh K, Fukuda M.  Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors.  J Cell Sci  2019  132(5). pii: jcs226977  PubMed ID: 30700496   DOI: 10.1242/jcs.226977

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User's Publication
21337  Komori T, Kuwahara T, Fujimoto T, Sakurai M, Koyama-Honda I, Fukuda M, Iwatsubo T.  Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2.  J Cell Sci  2023  136(14)  PubMed ID: 37365944   DOI: 10.1242/jcs.261003



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