Cell No. : Cell Name
RCB5388 : HeLaM
update : 2024/11/11
|
| Comment | |
| Comment from the depositor | Human cervical cancer cell line HeLaM (substrain of HeLa). |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
FUKUDA, Mitsunori
|
| Year of deposit |
2021
|
| Original cell |
HeLa
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
non-recombinant
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_R965
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
DMEM + 10% FBS
|
DMEM (high glucose, without Sodium pyruvate) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
(6-8x10 5 cells / 100mm dish) or (1 : 10-20)
|
| SC frequency |
|
Subculture : 2-3 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10%DMSO
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
3
|
| User's Publication |
1
|
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