Cell No. : Cell Name
RCB5379 : P3-X63-Ag8-U/hZC3H12D･TFL-21-7G
update : 2024/11/11
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| Comment | |
| Comment from the depositor | Mouse hybridoma cell line producing anti-human ZC3H12D(TFL) monoclonal antibody. |
| Terms and conditions | 1) The use of the BIOLOGICAL RESOURCE need sapre-approval letter by the developer, Dr.Toshimitsu Matsui (matsui.kobe@gnlail.com). 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Immunol. 2014:192(4):1512-1524) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KATAYAMA, Yoshio
|
| Originator |
MATSUI, Toshimitsu
|
| Year of deposit |
2021
|
| Another name |
anti-hZC3H12D･TFL//P3X63Ag8U1
|
| Cloning (depositor) |
Yes
|
| _Date (depositor) |
2009
|
| Animal |
_mouse x mouse
< Hybrid
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_A7BN
|
|
hybridoma
|
Fusion_cell_1 name |
P3-X63-Ag8-U
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
lymph node
|
| Fusion_cell_2 strain |
mouse, C57BL
|
| Fusion_cell_2 gender |
Unknown
|
|
Fusion method |
50% PEG
|
|
Antibody name |
hZC3H12D･TFL-21-7G
|
| Subclass |
IgG2b
|
| Immunogen |
Human TFLp36-GST fusion recombinant protein
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|
Antigen_name |
human TFL (ZC3H12D)p58 & p36
|
| Antigen_m.w. |
58/36k-Da
|
| Antigen_character |
Zinc finger (ZF) CCCH domain containing protein 12D (ZC3H12D/TFL/Regnase-4
|
| Antigen_epitope |
TFLp36 321 aa
|
|
Antigen_positive |
lymphocytes・spleen/lymph node
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI-1640+10%FCS
|
PMI1640 + 10% FBS
|
| Antibiotics |
penicillin, kanamycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 2-3 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG2b(Kappa)
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| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
15426
Tomita T, Kato M, Mishima T, Matsunaga Y, Sanjo H, Ito KI, Minagawa K, Matsui T, Oikawa H, Takahashi S, Takao T, Iwai N, Mino T, Takeuchi O, Maru Y, Hiratsuka S.
Extracellular mRNA transported to the nucleus exerts translation-independent function
Nat Commun
2021
12(1):3655
PubMed ID: 34135341
DOI: 10.1038/s41467-021-23969-1
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|
3252
Kentaro Minagawa, Kanako Wakahashi, Hiroki Kawano, Shinichiro Nishikawa, Chie Fukui, Yuko Kawano, Noboru Asada, Mari Sato, Akiko Sada, Yoshio Katayama, Toshimitsu Matsui
Posttranscriptional modulation of cytokine production in T cells for the regulation of excessive inflammation by TFL
J Immunol
2014
192(4):1512-24
PubMed ID: 24415781
DOI: 10.4049/jimmunol.1301619
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